Mutations in the YRB1 gene encoding yeast Ran-binding-protein-1 that impair nucleocytoplasmic transport and suppress yeast mating defects

Genetics

Volumn 157, Issue 3, 2001, Pages 1089-1105

  Künzler, Markus ^a,b   Trueheart, Joshua ^a,c   Sette, Claudio ^a   Hurt, Eduard ^b   Thorner, Jeremy ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b UNIVERSITY OF HEIDELBERG   (Germany)

^c Microbia PE Inc   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

GENE PRODUCT; GUANINE NUCLEOTIDE; GUANOSINE TRIPHOSPHATASE; NUCLEAR PROTEIN; RAN PROTEIN; RNA;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ANIMAL CELL; ARTICLE; CELL NUCLEUS MEMBRANE; CELLULAR DISTRIBUTION; CYTOSOL; EVOLUTION; GENE MUTATION; GENE REPRESSION; MATING; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN BINDING; SEQUENCE HOMOLOGY; TEMPERATURE SENSITIVE MUTANT; YEAST;

ADAPTOR PROTEINS, SIGNAL TRANSDUCING; ALLELES; AMINO ACID SEQUENCE; CARRIER PROTEINS; CELL NUCLEUS; CONSERVED SEQUENCE; CYTOPLASM; DOWN-REGULATION; ELECTROPHORESIS, POLYACRYLAMIDE GEL; EVOLUTION, MOLECULAR; FUNGAL PROTEINS; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTATION; NUCLEAR PROTEINS; PHENOTYPE; PLASMIDS; PROTEIN TRANSPORT; RAN GTP-BINDING PROTEIN; SACCHAROMYCES CEREVISIAE PROTEINS; SEQUENCE HOMOLOGY, AMINO ACID; TEMPERATURE; TWO-HYBRID SYSTEM TECHNIQUES;

ANIMALIA; FUNGI;

EID: 0035096793     PISSN: 00166731     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (99)

1. A yeast mutant, PRP20, altered in mRNA metabolism and maintenance of the nuclear structure, is defective in a gene homologous to the human gene RCC1, which is involved in the control of chromosome condensation
2. Kap104p: A karyopherin involved in the nuclear transport of messenger RNA binding proteins
3. Genetic relationships between the G protein βγ complex, Ste5p, Ste20p and Cdc42p: Investigation of effector roles in the yeast pheromone response pathway
4. The Saccharomyces cerevisiae RanGTP-binding protein Msn5p is involved in different signal transduction pathways
5. Isolation and characterization of RAT1: An essential gene of Saccharomyces cerevisiae required for the efficient nucleocytoplasmic trafficking of mRNA
6. Nuclear PRP20 protein is required for mRNA export
7. Characterization of an essential Saccharomyces cerevisiae gene related to RNA processing: Cloning of RNA1 and generation of a new allele with a novel phenotype
8. Nup116p and Nup100p are interchangeable through a conserved motif which constitutes a docking site for the mRNA transport factor Gle2p
9. Signal propagation and regulation in the mating pheromone pathway of the yeast Saccharomyces cerevisiae
10. Analyzing protein-protein interactions using the two-hybrid system
11. Yeast Ran-binding protein Yrb1p is required for efficient proteolysis of the cell cycle regulatory proteins Pds1p and Sic1p
12. The Ran/TC4 GTPase-binding domain: Identification by expression cloning and characterization of a conserved sequence motif
13. GSP1 and GSP2, genetic suppressors of the prp20-1 mutant in Saccharomyces cerevisiae: GTP-binding proteins involved in the maintenance of nuclear organization
14. Nuclear export of Far1p in response to pheromones requires the export receptor Msn5/Ste21p
15. 5-Fluoroorotic acid as a selective agent in yeast molecular genetics
16. The yeast nucleoporin Nup2p is involved in nuclear export of importin alpha/Srp1p
17. Sterile host yeasts (SHY): A eukaryotic system of biological containment for recombinant DNA experiments
18. A rapid and sensitive method for quantita-Lion of microgram quantities of protein utilizing the principle of protein-dye binding
19. Two differentially regulated mRNAs with different 5′ ends encode secreted and intracellular forms of yeast invertase
20. Yeast pheromone response pathway: Characterization of a suppressor that restores mating to receptorless mutants
21. Nucleocytoplasmic transport of macromolecules
22. A one-tube plasmid DNA mini-preparation suitable for sequencing
23. The nuclear exportin Msn5 is required for nuclear export of the Mig1 glucose repressor of Saccharomyces cerevisiae
24. A novel nuclear pore protein Nup133p with distinct roles in poly (A) + RNA transport and nuclear pore distribution
25. Routing MAP kinase cascades
26. A family of versatile centromerle vectors designed for use in the sectoring-shuffle mutagenesis assay in Saccharomyces cerevisiae
27. Identification of a yeast karyopherin heterodimer that targets import substrate to mammalian nuclear pore complexes
28. Allele-specific suppression of a Saccharomyces cerevisiae prp20 mutation by overexpression of a nuclear serine threonine protein kinase
29. Genetic and biochemical characterization of a phosphatidylinositol-specific phospholipase C in Saccharomyces cerevisiae
30. Site-directed mntagenesis using a double-stranded DNA fragment as a PCR primer
31. Improved method for high efficiency transformation of intact yeast cells
32. Transport between the cell nucleus and the cytoplasm
33. Studies on transformation of Escherichia coli with plasmids
34. Yeast Los1p has properties of an exportin-like nucleocytoplasmic transport factor for tRNA
35. GTP hydrolysis by Ran is required for nuclear envelope assembly
36. Yeast/E. coli shuttle vectors with multiple unique restriction sites
37. A novel in vivo assay reveals inhibition of ribosomal nuclear export in Ran-cycle and nucleoporin mutants
38. Mutational analysis of STE5 in the yeast Saccharomyces cerevisiae: Application of a differential interaction trap assay for examining protein-protein interactions
39. Ste5 RING-H2 domain: Role in Ste4-promoted oligomerization for yeast pheromone signaling
40. S. cerevisiae alpha pheromone receptors activate a novel signal transduction pathway for mating partner discrimination
41. Regulation of RNA processing and transport by a nuclear guanine nucleotide release protein and members of the Ras superfamily
42. Regulation of nuclear localization: A key to a door
43. The receptor Msn5 exports the phosphorylated transcription factor Pho4 out of the nucleus
44. Beyond nuclear transport. Ran-GTP as a determinant of spindle assembly
45. Temperature-sensitive lethal pseudorevertants of stemutations in Saccharomyces cerevisiae
46. Dynamic localization of the nuclear import receptor and its inter-actions with transport factors
47. Yeast NSR1 protein that has structural similarity to mammalian nucleolin is involved in pre-rRNA processing
48. Cse1p functions as the nuclear export receptor for importin a in yeast
49. Yeast Ran-binding protein 1 (Yrb1) shuttles between nucleus and cytoplasm and is exported from the nucleus via a CRMl(XPO1)-dependent pathway
50. Pheromone signalling and polarized morphogenesis in yeast
51. A protein that shuttles between the nucleus and the cytoplasm is an important mediator of RNA export
52. Interaction of a G-protein beta-subunit with a conserved sequence in Ste20/PAK family protein kinases
53. Cell-cycle checkpoints that ensure coordination between nuclear and cytoplasmic events in Saccharomyces cerevisiae
54. NUP2, a novel yeast nucleoporin, has functional overlap with other proteins of the nuclear pore complex
55. The yeast nuclear import receptor is required for mitosis
56. Ran
57. Cell polarity and morphogenesis in budding yeast
58. Nuclear shuttling of yeast scaffold Ste5 is required for its recruitment to the plasma membrane and activation of the mating MAPK cascade
59. Human RanBP3, a group of nuclear RanGTP binding proteins
60. Analysis of nucleo-cytoplasmic transport in a thermosensitire mutant of the nuclear pore protein NSP1
61. Nuclear protein import, but not mRNA export, is defective in all Saccharomyces cerevsiae mutants that produce temperature-sensitive forms of the Ran GTPase homologue Gsp1p
62. A RanBP1 mutation which does not visibly affect nuclear import may reveal additional functions of the Ran GTPase system
63. Ran-binding protein-1 is an essential component of the Ran/RCC1 molecular switch system in budding yeast
64. Improved tools for biological sequence analysis
65. Random mutagenesis and functional analysis of the Ran-binding protein, RanBP1
66. A bifunctional gene product involved in two phases of the yeast cell cycle
67. Facilitated nncleocytoplasmic shuttling of the Ran binding protein RanBP1
68. Membrane recruitment of the kinase cascade scaffold protein Ste5 by the Gβγ complex underlies activation of the yeast pheromone response pathway
69. The selection of S. cerevisiae mutants defective in the start event of cell division
70. Nuclear fusion in the yeast Saccharomyces cerevisiae
71. The nuclear pore complex: A protein machine bridging the nucleus and cytoplasm
72. The Ran decathlon: Multiple roles of Ran
73. Mutants in a yeast Ran binding protein are defective in nuclear transport
74. Yrb4p, a yeast Ran-GTP-binding protein involved in import of ribosomal protein L25 into the nucleus
75. Genetic evidence for interactions between yeast importin alpha (Srp1p) and its nuclear export receptor
76. Importin/karyopherin protein family members required for mRNA export from the nucleus
77. Mex67p which is an essential factor for nuclear mRNA export binds to both poly(A)+ RNA and nuclear pores
78. Mtr10p functions as a nuclear import receptor for the mRNA binding protein Npl3p
79. Nuclear sequestration of the exchange factor Cdc24 by Far1 regulates cell polarity during yeast mating
80. In vivo nuclear transport kinetics in Saccharomyces cerevisiae: A role for Hsp70 during targeting and translocation
81. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae
82. Assay of yeast mating reaction
83. Pheromone response and signal transduction during the mating process of Saccharomyces cerevisiae
84. Exportin 1 (Crm1p) is an essential nuclear export factor
85. MAP kinase signaling induces nuclear reorganization in budding yeast
86. Use of bacteriophage T7 lysozyme to improve an inducible T7 expression system
87. A member of the Ran-binding protein family, Yrb2p, is involved in nuclear protein export
88. CLUSTAL W: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice
89. The yeast cell fusion protein FUS1 is O-glycosylated and spans the plasma membrane
90. Structure of a Ran-binding domain complexed with Ran bound to a GTP analogue: Implications for nuclear transport
91. Interaction between the small GTPase Ran/Gsp1p and Ntf2p is required for nuclear transport
92. CSE1 and CSE2, two new genes required for accurate mitotic chromosome segregation in Saccharomyces cerevisiae
93. Crm1p mediates regulated nuclear export of a yeast AP-1-like transcription factor
94. A giant nucleopore protein that binds Ran/TC4
95. A phospholipase C-dependent inositol polyphosphate kinase pathway required for efficient messenger RNA export
96. Chromatin-independent nuclear envelope assembly induced by Ran GTPase in Xenopus egg extracts