Mutational analysis of STE5 in the yeast Saccharomyces cerevisiae: Application of a differential interaction trap assay for examining protein- protein interactions

Genetics

Volumn 147, Issue 2, 1997, Pages 479-492

  Inouye, Carla ^a   Dhillon, Namrita ^a,c   Durfee, Tim ^b   Zambryski, Patricia C ^b   Thorner, Jeremy ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

^c EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH AND HUMAN DEVELOPMENT   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

MITOGEN ACTIVATED PROTEIN KINASE;

ARTICLE; MATING; MISSENSE MUTATION; NONHUMAN; PRIORITY JOURNAL; PROTEIN PROTEIN INTERACTION; SACCHAROMYCES CEREVISIAE; SIGNAL TRANSDUCTION; STRUCTURE ACTIVITY RELATION;

ADAPTOR PROTEINS, SIGNAL TRANSDUCING; CARRIER PROTEINS; FUNGAL PROTEINS; POINT MUTATION; PROTEIN BINDING; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS;

SACCHAROMYCES CEREVISIAE;

EID: 0030777328     PISSN: 00166731     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (69)

1. AKADA, R., L. KALLAL, D. I. JOHNSON and J. KURJAN, 1996 Genetic relationships between the G protein βγ complex, Ste5p, Ste20p and Cdc42p: investigation of effector roles in the yeast pheromone response pathway. Genetics 143: 103-117.
2. ALLEN, J. B., M. W. WALBERG, M. C. EDWARDS and S. J. ELLEDGE, 1995 Finding prospective partners in the library: the two-hybrid system and phage display find a match. Trends Biochem. Sci. 20: 511-516.
3. BAI, C., and S. J. ELLEDGE, 1996 Gene identification using the yeast two-hybrid system. Methods Enzymol. 273: 331-347.
4. BARDWELL, L., J. G. COOK, C. J. INOUYE and J. THORNER, 1994 Signal propagation and regulation in the mating pheromone response pathway of the yeast Saccharomyces cerevisiae. Dev. Biol. 166: 363-379.
5. BARDWELL, L., J. G. COOK, E. C. CHANG, B. R. CAIRNS and J. THORNER, 1996 Signaling in the yeast pheromone response pathway: specific and high-affinity interaction of the mitogen-activated protein (MAP) kinases Kss1 and Fus3 with the upstream MAP kinase kinase Ste7. Mol. Cell. Biol. 16: 3637-3650.
6. BARNES, D. A., and J. THORNER, 1986 Genetic manipulation of Saccharomyces cerevisiae by use of the LYS2 gene. Mol. Cell. Biol. 6: 2828-2838.
7. BOEKE, J. D., F. LACROUTE and G. R. FINK, 1984 A positive selection for mutants lacking orotidine 5-phosphate decarboxylase activity in yeast: 5′-fluoro-orotic acid resistance. Mol. Gen. Genet. 197: 345-346.
8. BREEDEN, L., and K. NASMYTH, 1985 Regulation of the yeast HO gene. Cold Spring Harbor Symp. Quant. Biol. 50: 643-650.
9. BRILL, J. A., E. A. ELION and G. R. FINK, 1994 A role for autophosphorylation revealed by activated alleles of FUS3, the yeast MAP kinase homolog. Mol. Biol. Cell. 5: 297-312.
10. CAIRNS, B. R., S. W. RAMER and R. D. KORNBERG, 1992 Order of action of components in the yeast pheromone response pathway revealed with a dominant allele of the STE11 kinase and the multiple phosphorylation of the STE7 kinase. Genes Dev. 6: 1305-1318.
11. CHENEVERT, J., N. VALTZ and I. HERSKOWITZ, 1994 Identification of genes required for normal pheromone-induced cell polarization in Saccharomyces cerevisiae. Genetics 136: 1287-1297.
12. CHOI, K. Y., B. SATTERBERG, D. M. LYONS and E. A. ELION, 1994 Ste5 tethers multiple protein kinases in the MAP kinase cascade required for mating in S. cerevisiae. Cell. 78: 499-512.
13. CID, V. J., A. DURAN, F. DEL REY, M. P. SNYDER, C. NOMBELA et al., 1995 Molecular basis of cell integrity and morphogenesis in Saccharomyces cerevisiae. Microbiol. Rev. 59: 345-386.
14. COLE, G. M., and S. I. REED, 1991 Pheromone-induced phosphorylation of a G protein beta subunit in S. cerevisiae is associated with an adaptive response to mating pheromone. Cell 64: 703-716.
15. DURFEE, T., K. BECHERER, P. L. CHEN, S. H. YEH, Y. YANG et al., 1993 The retinoblastoma protein associates with the protein phosphatase type 1 catalytic subunit. Genes Dev. 7: 555-569.
16. ELION, E., 1995 Ste5: A meeting place for MAP kinases and their associates. Trends Cell Biol. 5: 322-327.
17. ELION, E. A., B. SATTERBERG and J. E. KRANZ, 1993 FUS3 phosphorylates multiple components of the mating signal transduction cascade: evidence for STE12 and FAR1. Mol. Biol. Cell. 4: 495-510.
18. ESTOJAK, J., R. BRENT and E. A. GOLEMIS, 1995 Correlation of two-hybrid affinity data with in vitro measurements. Mol. Cell. Biol. 15: 5820-5829.
19. EVAN, G. I., G. K. LEWIS, G. RAMSEY and J. M. BISHOP, 1985 Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product. Mol. Cell. Biol. 5: 3610-3616.
20. FIELDS, S., and I. HERSKOWITZ, 1985 The yeast STE12 product is required for expression of two sets of cell-type specific genes. Cell 42: 923-930.
21. FIELDS, S., and O. SONG, 1989 A novel genetic system to detect protein-protein interactions. Nature 340: 245-246.
22. GARTNER, A., K. NASMYTH and G. AMMERER, 1992 Signal transduction in Saccharomyces cerevisiae requires tyrosine and threonine phosphorylation of FUS3 and KSS1. Genes Dev. 6: 1280-1292.
23. GIETZ, R. D., and A. SUGINO, 1988 New yeast-Escherichia coli shuttle vectors constructed with in vitro mutagenized yeast genes lacking six-base pair restriction sites. Gene 74: 527-534.
24. HAGEN, D. C., G. MCCAFFREY and G. F. J. SPRAGUE, 1991 Pheromone response elements are necessary and sufficient for basal and pheromone-induced transcription of the FUS1 gene of Saccharomyces cerevisiae. Mol. Cell. Biol. 11: 2952-2961.
25. HASSON, M. S., 1992 Analysis of Saccharomyces cerevisiae pheromone response: biochemical and genetic characterization of the Ste5 protein. Ph.D. Thesis, University of California, Berkeley.
26. HASSON, M. S., D. BLINDER, J. THORNER and D. D. JENNESS, 1994 Mutational activation of the STE5 gene product bypasses the requirement for G protein β and γ subunits in the yeast pheromone response pathway. Mol. Cell. Biol. 14: 1054-1065.
27. HERSKOWITZ, I., 1995 MAP kinase pathways in yeast: lor mating and more. Cell 80: 187-197.
28. HIRSCHMAN, J. E., G. S. D. ZUTTKR, W. F. SIMONDS and D. J. JENNESS, 1996 The Gβγ complex of the yeast pheromone response pathway: subcelluar fractionation and protein-protein interactions. J. Biol. Chem. 272: 240-248.
29. INOUYE, C., N. DHILLON and J. THORNER, 1997 Ste5 RING-H2 domain: Role in Ste4-promoted oligomerization for yeast pheromone signaling. Science (in press).
30. JENNESS, D. D., and H. K. SCHACHMAN, 1983 Genetic characterization of the folding domains of the catalytic chains in aspartate transcarbamoylase. J. Biol. Chem. 258: 3266-3279.
31. JONES, E. W., 1991 Tackling the protease problem in Saccharomyces cerevisiae. Methods Enzymol. 194: 428-453.
32. KRANZ, J. E., B. SATTERBERG and E. A. ELION, 1994 The MAP kinase Fus3 associates with and phosphorylates the upstream signaling component Ste5. Genes Dev. 8: 313-327.
33. LEBERER, E., D. DIGNARD, D. HARCUS, D. Y. THOMAS and M. WHITEWAY, 1992 The protein kinase homologue Ste20p is required to link the yeast pheromone response G-protein βγ subunits to downstream signalling components. EMBO J. 11: 4815-4824.
34. LEBERER, E., D. DIGNARD, D. HARCUS, L. HOUGAN, M. WHITEWAY et al., 1993 Cloning of Saccharomyces cerrvisiae STE5 as a suppressor of a Ste20 protein kinase mutant: structural and functional similarity of Ste5 to Far1. Mol. Gen. Genet. 241: 241-254.
35. LEBERER, E., J. CHENEVERT, T. LEEUW, D. HARCUS, I. HERSKOWITZ et al., 1996 Genetic interactions indicate a role for Mdg1p and the SH3 domain protein Bem1p in linking the G-protein mediated yeast pheromone signalling pathway to regulators of cell polarity. Mol. Gen. Genet. 252: 608-621.
36. LEMMON, M. A., and J. SCHLESSINGER, 1994 Regulation of signal transduction and signal diversity by receptor oligomerization. Trends Biochem. Sci. 19: 459-463.
37. LYONS, D. M., S. K. MAHANTY, K. Y. CHOI, M. MANANDHAR and E. A. ELION, 1996 The SH3-domain protein Bem1 coordinates mitogen-activated protein kinase cascade activation with cell cycle control in Saccharomyces cerevisiae. Mol. Cell. Biol. 16: 4095-106.
38. MA, D., J. G. CooK and J. THORNER, 1995 Phosphorylation and localization of Kss1, a MAP kinase of the Saccharomyces cerevisiae pheromone response pathway. Mol. Biol. Cell 6: 889-909.
39. MACKAY, V. L., 1983 Cloning of yeast STE genes in 2 microns vectors. Methods Enzymol. 101: 325-343.
40. MANSER, E., T. LEUNG, H. SALIHUDDIN, Z. S. ZHAO and L. LIM, 1994 A brain serine/threonine protein kinase activated by Cdc42 and Racl. Nature 367: 40-46.
41. MARCUS, S., A. POLVERINO, M. BARR and M. WIGLER, 1994 Complexes between STE5 and components of the pheromone-responsive mitogen-activated protein kinase module. Proc. Natl. Acad. Sci. USA 91: 7762-7766.
42. MENDELSOHN, A. R., and R. BRENT, 1994 Applications of interaction traps/two-hybrid systems to biotechnology research. Curr. Opin. Biotechnol. 5: 482-486.
43. MILLER, J. H., 1972 Experiments in Molecular Genetics. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
44. MUHLRAD, D., R. HUNTER and R. PARKER, 1992 A rapid method for localized mutagenesis of yeast genes. Yeast 8: 79-82.
45. MUKAI, Y., S. HARASHIMA and Y. OSHIMA, 1993 Function of the Ste signal transduction pathway for mating pheromones sustains MATα1 transcription in Saccharomyces cerevisiae. Mol. Cell. Biol. 13: 2050-2060.
46. NEIMAN, A. M., and I. HERSKOWITZ, 1994 Reconstitution of a yeast protein kinase cascade in vitro: activation of the yeast MEK homologue STE7 by STE11. Proc. Natl. Acad. Sci. USA 91: 3398-3402.
47. PERLMAN, R., D. YABLONSKI, G. SIMCHEN and A. LEVITZKI, 1993 Cloning of the STE5 gene of Saccharomyces cerevisiae as a suppressor of the mating defect of cdc25 temperature-sensitive mutants. Proc. Natl. Acad. Sci. USA 90: 5474-5478.
48. PETER, M., and I. HERSKOWITZ, 1994 Direct inhibition of the yeast cyclin-dependent kinase Cdc28-Cln by Far1. Science 265: 1228-1231.
49. PHIZICKY, E. M., and S. FIELDS, 1995 Protein-protein interactions: methods for detection and analysis. Microbiol. Rev. 59: 94-123.
50. PRINTEN, J. A., and G. F. SPRAGUE, 1994 Protein-protein interactions in the yeast pheromone response pathway: Ste5p interacts with members of the MAP kinase cascade. Genetics 138: 609-619.
51. PRYCIAK, P. M., and L. H. HARTWELL, 1996 AKR1 encodes a candidate effector of the Gβγ complex in the Saccharomyces cerevisiae pheromone response pathway and contributes to control of both cell shape and signal transduction. Mol. Cell. Biol. 16: 2614-2626.
52. RAMER, S. W., and R. W. DAVIS, 1993 A dominant truncation allele identifies a gene, STE20, that encodes a putative protein kinase necessary for mating in Saccharomyces cerevisiae. Proc. Natl. Acad. Sci. USA 90: 452-456.
53. RHODES, N., L. CONNELL and B. ERREDE, 1990 STE11 is a protein kinase required for cell-type-specific transcription and signal transduction in yeast. Genes Dev. 4: 1862-1874.
54. SAMBROOK, J., E. F. FRITSCH and T. MANIATIS, 1989 Molecular Cloning: A Laboratory Manual, Ed. 2. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
55. SCHULTZ, J., B. FERGUSON and G. F. J. SPRAGUE, 1995 Signal transduction and growth control in yeast. Curr. Opin. Genet. Dev. 5: 31-37.
56. SIMON, M. N., C. DE VIRGILIO, B. SOUZA, J. R. PRINGLE, A. ABO et al., 1995 Role for the Rho-family GTPase Cdc42 in yeast mating-pheromone signal pathway. Nature 376: 702-705.
57. SONG, J., J. HIRSCHMAN, K. GUNN and H. G. DOHLMAN, 1996 Regulation of membrane and subunit interactions by N-myristoylation of a G protein a subunit in yeast. J. Biol. Chem. 271: 20273-20283.
58. SPRAGUE, G. F., JR., 1991 Assay of yeast mating reaction. Methods Enzymol. 194: 77-93.
59. SPRAGUE, G. F., and J. W. THORNER, 1992 Pheromone response and signal transduction during the mating process of Saccharomyces cerevisiae, pp. 657-744 in The Molecular and Cellular Biology of the Yeast Saccharomyces cerevisiae: Gene Expression. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
60. STEVENSON, B. J., N. RHODES, B. ERREDE and G. F. SPRAGUE, JR., 1992 Constitutive mutants of the protein kinase STE11 activate the yeast pheromone response pathway in the absence of the G protein. Genes Dev. 6: 1293-1304.
61. TYERS, M., and B. FUTCHER, 1993 Far1 and Fus3 link the mating pheromone signal transduction pathway to three G1-phase Cdc28 kinase complexes. Mol. Cell. Biol. 13: 5659-5669.
62. WHITE, M. A., 1996 The yeast two-hybrid system: forward and reverse. Proc. Natl. Acad. Sci. USA 93: 10001-10003.
63. WHITE, M. A., C. NICOLETTE, A. MINDEN, A. POLVERINO, L. VAN AELST et al., 1995 Multiple Ras functions can contribute to mammalian cell transformation. Cell 80: 533-541.
64. WHITEWAY, M. S., C. WU, T. LEEUW, K. CLARK, A. FOUREST-LIEUVIN et al., 1995 Association of the yeast pheromone response G protein βγ subunits with the MAP kinase scaffold Ste5p. Science 269: 1572-1575.
65. WU, C., M. WHITEWAY, D. Y. THOMAS and E. LEBERER, 1995 Molecular characterization of Ste20p, a potential mitogen-activated protein or extracellular signal-related kinase kinase (MEK) kinase kinase from Saccharomyces cerevisiae. J. Biol. Chem. 270: 15984-15992.
66. YABLONSKI, D., I. MARBACH and A. LEVITZKI, 1996 Dimerization of Ste5, a mitogen-activated protein kinase cascade scaffold protein, is required for signal transduction. Proc. Natl. Acad. Sci. USA 93: 13864-13869.
67. YASHAR, B., K. IRIE, J. A. PRINTEN, B. J. STEVENSON, G. F. SPRAGUE, JR. et al., 1995 Yeast MEK-dependent signal transduction: response thresholds and parameters affecting fidelity. Mol. Cell. Biol. 15: 6545-6553.
68. ZABIN, I., and M. R. VILLAREJO, 1975 Protein complementation. Annu. Rev. Biochem. 44: 295-313.
69. ZHAO, Z. S., T. LEUNG, E. MANSER and L. LIM, 1995 Pheromone signalling in Saccharomyces cerevisiae requires the small GTP-binding protein Cdc42p and its activator CDC24. Mol. Cell. Biol. 15: 5246-5257.