Interaction of a G-protein β-subunit with a conserved sequence in Ste20/PAK family protein kinases

Nature

Volumn 391, Issue 6663, 1998, Pages 191-195

  Leeuw, Thomas ^a   Wu, Cunle ^a   Schrag, Joseph D ^a   Whiteway, Malcolm ^a,b   Thomas, David Y ^a,b   Leberer, Ekkehard ^a,b  

^a NATIONAL RESEARCH COUNCIL CANADA   (Canada)

^b MCGILL UNIVERSITY   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

GUANINE NUCLEOTIDE BINDING PROTEIN; PHEROMONE; PROTEIN KINASE;

ARTICLE; CARBOXY TERMINAL SEQUENCE; MATING; NONHUMAN; PRIORITY JOURNAL; PROTEIN PROTEIN INTERACTION; SACCHAROMYCES CEREVISIAE; SIGNAL TRANSDUCTION;

ADAPTOR PROTEINS, SIGNAL TRANSDUCING; ANIMALS; CARRIER PROTEINS; FUNGAL PROTEINS; GTP-BINDING PROTEIN BETA SUBUNITS; GTP-BINDING PROTEIN GAMMA SUBUNITS; GTP-BINDING PROTEINS; HETEROTRIMERIC GTP-BINDING PROTEINS; MICE; PHEROMONES; PROTEIN BINDING; PROTEIN-SERINE-THREONINE KINASES; RATS; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; SIGNAL TRANSDUCTION;

EID: 0032495478     PISSN: 00280836     EISSN: None     Source Type: Journal    
DOI: 10.1038/34448     Document Type: Article

References (20)

1. Leberer, E., Thomas, D. Y. & Whiteway, M. Pheromone signalling and polarized morphogenesis in yeast. Curr. Opin. Genet. Dev. 7, 59-66 (1997).
2. Sells, M. A. & Chernoff, J. Emerging from the Pak: the p21-activated protein kinase family. Trends Cell Biol. 7, 162-167 (1997).
3. Peter, M., Neiman, A. M., Park, H.-O., Lohuizen, L. v. & Herskowitz, I. Functional analysis of the interaction between the small GTP binding protein Cdc42 and the Ste20 protein kinase in yeast. EMBO J. 15, 7046-7059 (1996).
4. Leberer, E. et al. Functional characterization of the Cdc42p binding domain of yeast Ste20p protein kinase. EMBO J. 16, 83-97 (1997).
5. Chang, F. & Herskowitz, I. Phosphorylation of FAR1 in response to α-factor: a possible requirement for cell-cycle arrest. Mol. Biol. Cell 3, 445-450 (1992).
6. Gartner, A., Nasmyth, K. & Ammerer, G. Signal transduction in Saccharomyces cerevisiae requires tyrosine and threonine phosphorylation of FUS3 and KSS1. Genes Dev. 6, 1280-1292 (1992).
7. Wu, C. et al. Activation of myosin-I by members of the Ste20p protein kinase family. J. Biol. Chem. 271, 31787-31790 (1996).
8. Whiteway, M. S. et al. Association of the yeast pheromone response G protein βγ subunits with the MAP kinase scaffold Ste5p. Science 269, 1572-1575 (1995).
9. Bagrodia, S., Taylor, S. J., Creasy, C. L., Chernoff, J. & Cerione, R. A. Identification of a mouse p21Cdc42/Rac activated kinase. J. Biol. Chem. 270, 22731-22737 (1995).
10. Cvrckova, F., Devirgilio, C., Manser, E., Pringle, J. R. & Nasmyth, K. Ste20-like protein kinases are required for normal localization of cell growth and for cytokinesis in budding yeast. Genes Dev. 9, 1817-1830 (1995).
11. Leberer, E., Dignard, D., Hougan, L., Thomas, D. Y. & Whiteway, M. Dominant-negative mutants of a yeast G-protein β subunit identify two functional regions involved in pheromone signaling. EMBO J. 13, 4805-4813 (1992).
12. Wall, M. A. et al. The structure of the G protein heterotrimer GJ α1 β1 γ2. Cell 83, 1047-1058 (1995).
13. Sondek, J., Bohm, A., Lambright, D. G., Hamm, H. E. & Sigler, P. B. Crystal structure of a G-protein βγ dimer at 2.1A resolution. Nature 379, 369-374 (1996).
14. Wu, C. L., Whiteway, M., Thomas, D. Y. & Leberer, E. Molecular characterization of Ste20p, a potential mitogen-activated protein or extracellular signal-regulated kinase kinase (MEK) kinase kinase from Saccharomyces cerevisiae. J. Biol. Chem. 270, 15984-15992 (1995).
15. Leberer, E. et al. Cloning of Saccharomyces cerevisiae STE5 as a suppressor of a Ste20 protein kinase mutant: structural and functional similarity of Ste5 to Far1. Mol. Gen. Genet. 241, 241-254 (1993).
16. Sikorski, R. S. & Hieter, P. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics 122, 19-27 (1989).
17. Leberer, E., Dignard, D., Harcus, D., Thomas, D. Y. & Whiteway, M. The protein kinase homologue Ste20p is required to link the yeast pheromone response G-protein βγ subunits to downstream signalling components. EMBO J. 11, 4815-4824 (1992).
18. Kunkel, T. D., Roberts, J. D. & Zakour, R. A. Rapid and efficient site-specific mutagenesis without phenotypic selection. Meth. Enzymol. 154, 367-382 (1987).
19. Leeuw, T. et al. Pheromone response in yeast: association of Bem1p with proteins of the MAP kinase cascade and actin. Science 270, 1210-1213 (1995).
20. Manser, E., Leung, T., Salihuddin, H., Zhao, Z. S. & Lim, L. A brain serine/threonine protein kinase activated by Cdc42 and Rac1. Nature 367, 40-46 (1994).