Reconstitution of human β-globin locus control region hypersensitive sites in the absence of chromatin assembly

Molecular and Cellular Biology

Volumn 21, Issue 8, 2001, Pages 2629-2640

  Leach, K M ^a   Nightingale, K ^a   Igarashi, K ^a   Levings, P P ^a   Engel, J D ^a   Becker, P B ^a   Bungert, J ^a  

^a UNIVERSITY OF FLORIDA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BETA GLOBIN; DEOXYRIBONUCLEASE I; DNA; NUCLEASE S1; PERMANGANATE POTASSIUM; RNA POLYMERASE II;

ANIMAL CELL; ARTICLE; CELL SPECIFICITY; CHROMATIN STRUCTURE; CONTROLLED STUDY; DROSOPHILA; ENHANCER REGION; ERYTHROID CELL; ERYTHROLEUKEMIA CELL; HELA CELL; HUMAN; HUMAN CELL; IN VITRO STUDY; MOUSE; NONHUMAN; PRIORITY JOURNAL; REGULATOR GENE; TRANSCRIPTION INITIATION;

ANIMALS; BASE SEQUENCE; BINDING SITES; CELL LINE; CHROMATIN; CLONING, MOLECULAR; DEOXYRIBONUCLEASE I; DNA PRIMERS; ENHANCER ELEMENTS (GENETICS); ERYTHROCYTES; GLOBINS; HELA CELLS; HUMANS; LOCUS CONTROL REGION; MICE; TRANSCRIPTION, GENETIC;

ANIMALIA; MUS MUSCULUS;

EID: 0035076274     PISSN: 02707306     EISSN: None     Source Type: Journal    
DOI: 10.1128/MCB.21.8.2629-2640.2001     Document Type: Article

References (45)

1. NF-E2 disrupts chromatin structure at β-globin locus control region hypersensitive site 2 in vitro
2. A SWI/SNF-related chromatin remodeling complex, E-RC1, is required for tissue-specific transcriptional regulation by EKLF in vitro
3. Intergenic transcription and transinduction of the human β-globin locus
4. Cell-free system for assembly of transcriptionally repressed chromatin from Drosophila embryos
5. Globin gene switching and DNase I sensitivity of the endogenous β-globin locus in mice does not require the locus control region
6. Conservation of sequence and structure flanking the mouse and human beta-globin loci: The beta-globin genes are embedded within an array of odorant receptor genes
7. Looping versus linking: Toward a model for long-distance gene activation
8. Transcription factor eUSF is an essential component of isolated transcription complexes on the duck histone H5 gene and it mediates the interaction of TFIID with a TATA-deficient promoter
9. Synergistic regulation of human β-globin gene switching by locus control region elements HS3 and HS4
10. Hypersensitive site 2 specifies a unique function within the human β-globin locus control region to stimulate globin gene transcription
11. Conserved E-boxes function as part of the enhancer in hypersensitive site 2 of the beta-globin locus control region: Role of basic helix-loop-helix proteins
12. Looping, linking and chromatin activity: New insights into beta-globin locus regulation
13. Chromatin as an essential part of the transcriptional mechanism
14. Evidence for a locus activation region: The formation of developmentally stable hypersensitive sites in globin-expressing hybrids
15. Physical mapping of yeast artificial chromosomes containing sequences from the human β-globin gene region
16. Essential role of NF-E2 in remodeling of chromatin structure and transcriptional activation of the epsilon globin gene by 5′ hypersensitive site 2 of the β-globin locus control region
17. Intergenic transcription and developmental remodeling of chromatin subdomains in the human β-globin locus
18. Position-independent, high level expression of the human β-globin gene in transgenic mice
19. Activation by locus control regions?
20. Locus control regions of mammalian beta-globin gene clusters: Combining phylogenetic analyses and experimental results to gain functional insights
21. Do LCRs open chromatin domains?
22. Analysis of mice containing a targeted deletion of β-globin locus control region hypersensitive site 3
23. Sequences within and flanking hypersensitive sites 3 and 2 of the β-globin locus control region required for synergistic versus additive interaction with the ε-globin gene promoter
24. ATP-dependent positive supercoiling of DNA by 13S condensins: A biochemical implication for chromosome condensation
25. Transcription of the HS2 enhancer toward a cis-linked gene is independent of the orientation, position, and distance of the enhancer relative to the gene
26. Promoter-proximal pausing of RNA polymerase II defines a general rate-limiting step after transcription initiation
27. The β-globin promoter is important for recruitment of erythroid Krüppel-like factor to the locus control region in erythroid cells
28. Individual LCR hypersensitive sites cooperate to generaté an open chromatin domain spanning the human β-globin locus
29. Displacement of sequence-specific transcription factors from mitotic chromatin
30. Functional synergy and physical interactions of the erythroid transcription factor GATA-1 with the Krüppel family proteins Sp1 and EKLF
31. Marking of active genes on mitotic chromosomes
32. Heterochromatin effects on the frequency and duration of LCR-mediated gene transcription
33. Developmental specificity of the interaction between the locus control region and embryonic or fetal globin genes in transgenic mice with an HS3 core deletion
34. A DNA-binding factor in adult hematopoietic cells interacts with a pyrimidinerich domain upstream from the human delta-globin gene
35. Conserved elements containing NF-E2 and tandem GATA binding sites are required for erythroid-specific chromatin structure reorganization within the human β-globin locus control region
36. The locus control region is necessary for gene expression in the human β-globin locus but not for the maintenance of an open chromatin structure in erythroid cells
37. The role of general initiation factors in transcription by RNA polymerase II
38. Transcriptional repression by nucleosomes but not H1 in reconstituted preblastoderm drosophila chromatin
39. Hemoglobin switching
40. NF-E2 and GATA binding motifs are required for the formation of DNase I hypersensitive site 4 of the human beta-globin locus control region
41. The "β-like globin gene domain" in human erythroid cells
42. The LIM-only protein Lmo2 is a bridging molecule assembling an erythroid, DNA-binding complex which includes the Tall, E47, GATA-1 and Ldb1/NL1 proteins
43. Transcription complex stability and chromatin dynamics in vivo
44. The role of EKLF in human β-globin gene competition
45. Long range interaction of cis-DNA elements mediated by architectural transcription factor Bach 1