Telomerase expression prevents replicative senescence but does not fully reset mRNA expression patterns in Werner syndrome cell strains

FASEB Journal

Volumn 15, Issue 6, 2001, Pages 1014-1020

  Choi, Donghee ^a   Whittier, Peter S ^a   Oshima, Junko ^b   Funk, Walter D ^a  

^a GERON CORPORATION   (United States)

^b UNIVERSITY OF WASHINGTON   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DNA; MESSENGER RNA; TELOMERASE;

AGING; ARTICLE; AUTOSOMAL RECESSIVE DISORDER; DNA REPLICATION; FIBROBLAST CULTURE; GENE EXPRESSION; GENE MUTATION; HUMAN; HUMAN CELL; PRIORITY JOURNAL; SENESCENCE; TELOMERE; TRANSGENE; WERNER SYNDROME;

CELL AGING; CELLS, CULTURED; DNA-BINDING PROTEINS; GENE EXPRESSION; GENE EXPRESSION PROFILING; HUMANS; RNA; RNA, MESSENGER; SIGNAL TRANSDUCTION; TELOMERASE; WERNER SYNDROME;

EID: 0035069410     PISSN: 08926638     EISSN: None     Source Type: Journal    
DOI: 10.1096/fj.00-0104com     Document Type: Article

References (39)

1. Positional cloning of the Werner's syndrome gene
2. The Werner mutation: Does it lead to a "public" or "private" mechanism of aging?
3. A deletion within the murine Werner syndrome helicase induces sensitivity to inhibitors of topoisomerase and loss of cellular proliferative capacity
4. Cellular Werner phenotypes in mice expressing a putative dominant-negative human WRN gene
5. Mutations in the consensus helicase domains of the Werner syndrome gene
6. Impaired nuclear localization of defective DNA helicases in Werner's syndrome
7. The Werner syndrome protein is a DNA helicase
8. The premature ageing syndrome protein, WRN, is a 3′→5′ exonuclease
9. Werner syndrome protein. II. Characterization of the integral 3′→5′ DNA exonuclease
10. Werner syndrome helicase contains a 5′→3′ exonuclease activity that digests DNA and RNA strands in DNA/DNA and RNA/DNA duplexes dependent on unwinding
11. Functional and physical interaction between WRN helicase and human replication protein A
12. The Werner syndrome gene product co-purifies with the DNA replication complex and interacts with PCNA and topoisomerase I
13. The Werner syndrome protein is involved in RNA polymerase II transcription
14. Variegated translocation mosaicism in human skin fibroblast cultures
15. Cytogenetics of Werner's syndrome cultured skin fibroblasts: Variegated translocation mosaicism
16. Clonal structural chromosomal rearrangements in primary fibroblast cultures and in lymphocytes of patients with Werner's syndrome
17. Spontaneous and induced chromosomal instability, in Werner syndrome
18. DNA repair fine structure in Werner's syndrome cell lines
19. Systematic growth studies, cocultivation, and cell hybridization studies of Werner syndrome cultured skin fibroblasts
20. Accelerated loss of telomeric repeats may not explain accelerated replicative decline of Werner syndrome cells
21. Telomerase prevents the accelerated cell ageing of Werner syndrome fibroblasts
22. The establishment of telomerase-immortalized cell lines representing human chromosome instability syndromes
23. Microarray analysis of replicative senescence
24. Homozygous and compound heterozygous mutations at the Werner syndrome locus
25. Extension of life-span by introduction of telomerase into normal human cells
26. A biomarker that identifies senescent human cells in culture and in aging skin in vivo
27. A DNA microarray system for analyzing complex DNA samples using two-color fluorescent probe hybridization
28. Nucleolar localization of the Werner syndrome protein in human cells
29. Telomere length predicts replicative capacity of human fibroblasts
30. Mild hyperoxia shortens telomeres and inhibits proliferation of fibroblasts: A model for senescence?
31. Accumulation of single-strand breaks is the major cause of telomere shortening in human fibroblasts
32. Genetic effects on the longevity of cultured human fibroblasts. I. Werner's syndrome
33. Identification of gene sequences overexpressed in senescent and Werner syndrome human fibroblasts
34. Mismatch repair in extracts of Werner syndrome cell lines
35. Telomerase expression restores dermal integrity to in-vitro-aged fibroblasts in a reconstituted skin model
36. Werner's syndrome: Proliferation in vitro of clones of cells bearing chromosome translocations
37. Mutator phenotype of Werner syndrome is characterized by extensive deletions
38. A longitudinal study of human age-related chromosomal analysis in skin fibroblasts
39. Failure to complement abnormal phenotypes of simian virus 40-transformed Werner syndrome cells by introduction of a normal human chromosome 8