Adenovirus-mediated wt-p16 reintroduction induces cell cycle arrest or apoptosis in pancreatic cancer

Cancer Gene Therapy

Volumn 8, Issue 10, 2001, Pages 740-750

  Calbó, Joaquim ^a   Marotta, Mario ^a   Cascalló, Manel ^a   Roig, Josep Maria ^a   Gelpí, Josep Lluis ^a   Fueyo, Juan ^b   Mazo, Adela ^a  

^a UNIVERSITY OF BARCELONA   (Spain)

^b UNIVERSITY OF TEXAS   (United States)

Author keywords

Adenovirus; Apoptosis; p16; Pancreatic cancer; Senescence

Indexed keywords

ANTINEOPLASTIC AGENT; CYCLIN DEPENDENT KINASE; CYCLIN DEPENDENT KINASE 4; CYCLIN DEPENDENT KINASE 6; GENE PRODUCT; PROTEIN P16; TRANSCRIPTION FACTOR E2F; TRANSCRIPTION FACTOR E2F1; UNCLASSIFIED DRUG;

ADENOVIRUS; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; APOPTOSIS; ARTICLE; CANCER CELL CULTURE; CONTROLLED STUDY; GENE INACTIVATION; GENE OVEREXPRESSION; GENE THERAPY; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; MITOSIS INHIBITION; NONHUMAN; PANCREAS CANCER; PRIORITY JOURNAL; SENESCENCE; TUMOR SUPPRESSOR GENE; VIRUS RECOMBINANT;

ADENOCARCINOMA; ADENOVIRIDAE; ANIMALS; APOPTOSIS; BETA-GALACTOSIDASE; BLOTTING, WESTERN; BROMODEOXYURIDINE; CELL AGING; CELL CYCLE; CYCLIN-DEPENDENT KINASE INHIBITOR P16; GENE THERAPY; GENETIC VECTORS; HUMANS; MICE; MICE, INBRED BALB C; MICE, NUDE; PANCREATIC NEOPLASMS; TRANSFECTION; TUMOR CELLS, CULTURED;

ADENOVIRIDAE; ANIMALIA;

EID: 0034774666     PISSN: 09291903     EISSN: None     Source Type: Journal    
DOI: 10.1038/sj.cgt.7700374     Document Type: Article

References (57)

1. Cancer statistics, 1998
2. Cancer of the pancreas
3. Novel clinical strategies for the treatment of pancreatic carcinoma
4. Gene therapy for cancer: What have we done and where are we going?
5. Gene therapy for cancer
6. Cancer gene therapy: Hard lessons and new courses
7. Cell cycle and apoptosis: Common pathways to life and death
8. Gene therapy for cancer
9. Cell death and cancer: Replacement of apoptotic genes and inactivation of death suppressor genes in therapy
10. The Pezcoller lecture: Cancer cell cycles revisited
11. The INK4a/ARF tumor suppressor: One gene-two products-two pathways
12. Mdm-2: A bridge over the two tumour suppressors, p53 and pRb
13. The retinoblastoma protein and cell cycle control
14. Functional inactivation of the retinoblastoma protein requires sequential modification by at least two distinct cyclin-cdk complexes
15. Cell cycle control in mammalian cells: Role of cyclins, cyclin-dependent kinases (CDKs), growth suppressor genes and cyclin-dependent kinase inhibitors (CKIs)
16. 1-phase progression
17. Role of the retinoblastoma protein family, pRB, p107 and p130 in the negative control of cell growth
18. E2Fs regulate the expression of genes involved in differentiation, development, proliferation, and apoptosis
19. INK4A as a human tumor suppressor
20. Frequent somatic mutations and homozygous deletions of the MTS1 gene in pancreatic adenocarcinoma
21. A new regulatory motif in cell cycle control causing specific inhibition of cyclin D/CDK4
22. Retinoblastoma-protein-dependent cell cycle inhibition by the tumour suppressor p16
23. Involvement of the cyclin-dependent kinase inhibitor p16 (INK4a) in replicative senescence of normal human fibroblasts
24. INK4a enrichment and the premature senescence of normal fibroblasts
25. INK4A
26. Ink4a
27. INK4A adenovirusmediated gene therapy for human head and neck squamous cell cancer
28. INK4A expression on cell cycle arrest are determined by endogenous p16 and Rb status in human cancer cells
29. INK4A
30. Adenoviral vector containing wild-type p16 suppresses prostate cancer growth and prolongs survival by inducing cell senescence
31. Adenovirusmediated p16/CDKN2 gene transfer induces growth arrest and modifies the transformed phenotype of glioma cells
32. Restoration of wildtype p16 down-regulates vascular endothelial growth factor expression and inhibits angiogenesis in human gliomas
33. Adenovirusmediated p16/CDKN2 gene transfer suppresses glioma invasion in vitro
34. INK4 mediates contact inhibition of growth
35. INK4a tumour suppressor protein inhibits alpha-v-beta-3 integrin-mediated cell spreading on vitronectin by blocking PKC-dependent localization of alpha-v-beta-3 to focal contacts
36. KIP1 in inducing cell cycle arrest, apoptosis and inhibition of tumorigenicity
37. INK4a in mesothelioma cells results in cell cycle arrest, cell death, tumor suppression and tumor regression
38. High concentrations of recombinant adenovirus expressing p 16 gene induces apoptosis in lung cancer lines
39. INK4a results in pancreatic cancer regression in vitro and in vivo
40. Disruption of the antiproliferative TGF-β signaling pathways in human pancreatic cells
41. Genetic background determines the response to adenovirus-mediated wt-p53 expression in human pancreatic tumor cells
42. Analysis of cell death by flow cytometry
43. A biomarker that identifies senescent human cells in culture and aging skin in vivo
44. Modulation of drug cytotoxicity by reintroduction of wild-type p53 gene (Ad5CMV-p53) in human pancreatic cancer
45. Use of recombinant adenovirus for metabolic engineering of mammalian cells
46. Pre-existing immunity to adenovirus does not prevent tumor regression following intratumoral administration of a vector
47. The potential for gene therapy in pancreatic cancer
48. Strategies for targeting therapeutic gene delivery
49. Replacement of the p16/CDKN2 gene suppresses human glioma cell growth
50. The retinoblastoma protein pathway in cell cycle control and cancer
51. Adenovirally transferred p16 and p53 genes cooperate to induce apoptotic tumor cell death
52. INK4-mediated apoptosis in non-small cell lung cancer cells
53. Expression of p16 induces transcriptional downregulation of the RB gene
54. E2F-1-induced p53-independent apoptosis in transgenic mice
55. p16/MTS1/INK4A suppresses prostate cancer by both pRb-dependent and -independent pathways
56. 1 fibronectin receptor
57. The evaluation of adenoviral p53-mediated bystander effect in gene therapy of cancer