Binding of the Mex67p/Mtr2p heterodimer to FXFG, GLFG, and FG repeat nucleoporins is essential for nuclear mRNA export

Journal of Cell Biology

Volumn 150, Issue 4, 2000, Pages 695-706

  Sträßer, Katja ^a   Baßler, Jochen ^a   Hurt, Ed ^a,b  

^a Biochemie Zentrum Heidelberg   (Germany)

^b UNIVERSITY OF HEIDELBERG   (Germany)

Author keywords

Nuclear pore complex; Nucleocytoplasmic transport; Nup116; Nup159; Rip1

Indexed keywords

CARRIER PROTEIN; DIMER; MESSENGER RNA; NUCLEOPORIN;

ARTICLE; BINDING COMPETITION; COMPLEX FORMATION; MOLECULAR MODEL; PRIORITY JOURNAL; PROTEIN ASSEMBLY; PROTEIN DOMAIN; PROTEIN LOCALIZATION; PROTEIN PROTEIN INTERACTION; RNA TRANSPORT;

BINDING SITES; CELL NUCLEUS; CLONING, MOLECULAR; CROSSES, GENETIC; DIMERIZATION; FUNGAL PROTEINS; NUCLEAR ENVELOPE; NUCLEAR PROTEINS; NUCLEOCYTOPLASMIC TRANSPORT PROTEINS; RECOMBINANT FUSION PROTEINS; RECOMBINANT PROTEINS; RNA, MESSENGER; RNA-BINDING PROTEINS; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS;

EID: 0034698683     PISSN: 00219525     EISSN: None     Source Type: Journal    
DOI: 10.1083/jcb.150.4.695     Document Type: Article

References (75)

1. Nup120p: A yeast nucleoporin required for NPC distribution and mRNA transport
2. The C-terminal domain of TAP interacts with the nuclear pore complex and promotes export of specific CTE-bearing RNA substrates
3. Nup116p and Nup100p are interchangeable through a conserved motif which constitutes a docking site for the mRNA transport factor Gle2p
4. Nup116p associates with the Nup82p-Nsp1p-Nup159p nucleoporin complex
5. Nup84, a novel nucleoporin that is associated with CAN/Nup214 on the cytoplasmic face of the nuclear pore complex
6. Identification of novel import and export signals of human TAP, the protein that binds to the constitutive transport element of the type D retrovirus mRNAs
7. Functional characterization of a Nup159p-containing nuclear pore sub-complex
8. + RNA
9. TAP binds to the constitutive transport element (CTE) through a novel RNA-binding motif that is sufficient to promote CTE-dependent RNA export from the nucleus
10. + RNA export and in the cytoskeleton
11. The 1.6 Å resolution crystal structure of nuclear transport factor 2 (NTF2)
12. Dissecting the interactions between NTF2, RanGDP, and the nucleoporin XFXFG repeats
13. Separate binding sites on nuclear transport factor 2 (NTF2) for GDP-Ran and the phenylalanine-rich repeat regions of nucleoporins p62 and Nsp1p
14. A structure/function analysis of Rat7p/Nup159p, an essential nucleoporin of Saccharomyces cerevisiae
15. C-terminal truncations of the yeast nucleoporin Nup145p produce a rapid temperature-conditional mRNA export defect and alterations to nuclear structure
16. + RNA transport and nuclear pore distribution
17. Nup145p is required for nuclear export of mRNA and binds homopolymeric RNA in vitro via a novel conserved motif
18. Nucleocytoplasmic trafficking: What role for repeated motifs in nucleoporins?
19. Molecular architecture of the yeast nuclear pore complex: Localization of Nsp1p subcomplexes
20. The human homologue of yeast Crm1 is in a dynamic subcomplex with Can/Nup214 and a novel nuclear pore component Nup88
21. Pleiotropic nuclear defects associated with a conditional allele of the novel nucleoporin Rat9p/Nup85p
22. A conditional allele of the novel repeat-containing yeast nucleoporin RAT7/NUP159 causes both rapid cessation of mRNA export and reversible clustering of nuclear pore complexes
23. A novel nuclear pore protein Nup82p which specifically binds to a fraction of Nsp1p
24. TAP, the human homolog of Mex67p, mediates CTE-dependent RNA export from the nucleus
25. + RNA associated with mutation of the Saccharomyces cerevisiae RAT2/NUP120 gene
26. Yeast Los1p has properties of an exportin-like nucleocytoplasmic transport factor for tRNA
27. Rat8p/Dbp5p is a shuttling transport factor that interacts with Rat7p/Nup159p and Gle1p and suppresses the mRNA export defect of xpo1-1 cells
28. A novel in vivo assay reveals inhibition of ribosomal nuclear export in ran-cycle and nucleoporin mutants
29. Mex67p mediates the nuclear export of a variety of Pol II transcripts
30. -RNA export
31. Two yeast nuclear pore complex proteins involved in mRNA export form a cytoplasmically oriented subcomplex
32. A nuclear export signal in Kap95p is required for both recycling the import factor and interaction with the nucleoporin GLFG repeat regions of Nup116p and Nup100p
33. The GLFG repetitive region of the nucleoporin Nup116p interacts with Kap95p, an essential yeast nuclear import factor
34. The human Tap protein is a nuclear mRNA export factor that contains novel RNA-binding and nucleocytoplasmic transport sequences
35. The Mex67p-mediated nuclear mRNA export pathway is conserved from yeast to human
36. mRNA binding protein mrnp 41 localizes to both nucleus and cytoplasm
37. The essential yeast nucleoporin NUP159 is located on the cytoplasmic side of the nuclear pore complex and serves in karyopherin-mediated binding of transport substrate
38. Cse1p functions as the nuclear export receptor for importin α in yeast
39. Structure of bovine blood coagulation factor Va. Determination of the subunit associations, molecular weights and asymmetries by analytical ultracentrifugation
40. Nucleocytoplasmic transport: The soluble phase
41. GLE2, a Saccharomyces cerevisiae homologue of the Schizosaccharomyces pombe export factor RAE1, is required for nuclear pore complex structure and function
42. Transport of proteins and RNAs in and out of the nucleus
43. The NES-Crm1p export pathway is not a major mRNA export route in Saccharomyces cerevisiae
44. Nucleocytoplasmic transport: The last 200 nanometers
45. The constitutive transport element (CTE) of Mason-Pfizer monkey virus (MPMV) accesses a cellular mRNA export pathway
46. Reconstituted nuclei depleted of a vertebrate GLFG nuclear pore protein, p97, import but are defective in nuclear growth and replication
47. RAE1 is a shuttling mRNA export factor that binds to a GLEBS-like NUP98 motif at the nuclear pore complex through multiple domains
48. The peptide repeat domain of nucleoporin Nup98 functions as a docking site in transport across the nuclear pore complex
49. Protein import into nuclei: Association and dissociation reactions involving transport substrate, transport factors, and nucleoporins
50. The yeast nuclear pore complex: Composition, architecture, and transport mechanism
51. Yeast heat shock mRNAs are exported through a distinct pathway defined by Rip1p
52. Nuclear mRNA export requires complex formation between Mex67p and Mtr2p at the nuclear pores
53. Dbp5, a DEAD-box protein required for mRNA export, is recruited to the cytoplasmic fibrils of nuclear pore complex via a conserved interaction with CAN/Nup159p
54. + RNA and nuclear pores
55. Mtr10p functions as a nuclear import receptor for the mRNA binding protein Np13p
56. A novel complex of nucleoporins, which includes Sec13p and a Sec13p homolog, is essential for normal nuclear pores
57. A novel complex of membrane proteins required for formation of a spherical nucleus
58. Structure and assembly of the Nup84p complex
59. Dbp5p/Rat8p is a yeast nuclear pore-associated DEAD-box protein essential for RNA export
60. Exportin 1 (Crm1p) is an essential nuclear export factor
61. The nuclear pore complex: From molecular architecture to functional dynamics
62. The RNA export factor Gle1p is located on the cytoplasmic fibrils of the NPC and physically interacts with the FG-nucleoporin Rip1p, the DEAD-box protein Rat8p/Dbp5p and a new protein Ymr255p
63. Nuclear RNA export in yeast
64. Yra1p, a conserved nuclear RNA binding protein, interacts directly with Mex67p and is required for mRNA export
65. The yeast nucleoporin Rip1p contributes to multiple export pathways with no essential role for its FG-repeat region
66. The human tap nuclear RNA export factor contains a novel transportin-dependent nuclear localizatoin signal that lacks nuclear export signal function
67. + RNA export
68. A temperature-sensitive NUP116 null mutant forms a nuclear envelope seal over the yeast nuclear pore complex thereby blocking nucleocytoplasmic traffic
69. NUP145 encodes a novel yeast glycine-leucine-phenylalanine-glycine (GLFG) nucleoporin required for nuclear envelope structure
70. A new family of yeast nuclear pore complex proteins
71. A new subclass of nucleoporins that functionally interacts with nuclear pore protein NSP1
72. Approaches towards a genetic analysis of the nuclear pore complex in yeast
73. Crm1p mediates regulated nuclear export of a yeast AP-1-life transcription factor
74. Nucleoporins Nup98 and Nup214 participate in nuclear export of human immunodeficiency virus type 1