The RNA export factor Gle1p is located on the cytoplasmic fibrils of the NPC and physically interacts with the FG-nucleoporin Rip1p, the DEAD-box protein Rat8p/Dbp5p and a new protein Ymr255p

EMBO Journal

Volumn 18, Issue 20, 1999, Pages 5761-5777

  Strahm, Yvan ^a   Fahrenkrog, Birthe ^b   Zenklusen, Daniel ^a   Rychner, Elizabeth ^a   Kantor, Julia ^c   Rosbash, Michael ^c   Stutz, Françoise ^a  

^a LAUSANNE UNIVERSITY HOSPITAL   (Switzerland)

^b UNIVERSITY OF BASEL   (Switzerland)

^c BRANDEIS UNIVERSITY   (United States)

Author keywords

DEAD box protein; FG nucleoporin; Nuclear pore complex; RNA export; Yeast

Indexed keywords

CARRIER PROTEIN; CYTOPLASM PROTEIN; NUCLEOPORIN; RNA BINDING PROTEIN;

ARTICLE; CARBOXY TERMINAL SEQUENCE; GENETIC CONSERVATION; HUMAN; IMMUNOELECTRON MICROSCOPY; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN PROTEIN INTERACTION; RNA TRANSPORT; SEQUENCE HOMOLOGY; YEAST;

ADENOSINE TRIPHOSPHATASES; AMINO ACID SEQUENCE; ANIMALS; BASE SEQUENCE; CARRIER PROTEINS; CYTOPLASM; DEAD-BOX RNA HELICASES; DNA PRIMERS; FUNGAL PROTEINS; HUMANS; MICE; MICROSCOPY, IMMUNOELECTRON; MOLECULAR SEQUENCE DATA; MUTATION; NUCLEAR ENVELOPE; NUCLEAR PORE COMPLEX PROTEINS; NUCLEAR PROTEINS; NUCLEOCYTOPLASMIC TRANSPORT PROTEINS; RNA HELICASES; RNA-BINDING PROTEINS; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; SEQUENCE HOMOLOGY, AMINO ACID; SUPPRESSION, GENETIC; TEMPERATURE;

EID: 0033569797     PISSN: 02614189     EISSN: None     Source Type: Journal    
DOI: 10.1093/emboj/18.20.5761     Document Type: Article

References (48)

1. Ausubel, F.M., Brent, R., Kingston, R.E., Moore, D.D., Seidman, J.G., Smith, J.A. and Strahl, K. (1994) Current Protocols in Molecular Biology. J.Wiley & Sons and Greene Publishing Associates.
2. Belgareh, N., Snay-Hodge, C., Pasteau, F., Dagher, S., Cole, C.N. and Doye, V. (1998) Functional characterization of a Nup159p-containing nuclear pore subcomplex. Mol. Biol. Cell, 9, 3475-3492.
3. Bogerd, H.P., Fridell, R.A., Madore, S. and Cullen, B.R. (1995) Identification of a novel cellular cofactor for the Rev/Rex class of retroviral regulatory proteins. Cell, 82, 485-494.
4. Dahlberg, J.E. and Lund, E. (1998) Functions of the GTPase Ran in RNA export from the nucleus. Curr. Opin. Cell Biol., 10, 400-408.
5. de Castillia, C.S. and Rout, M.P. (1999) TAPping into transport. Nature Cell Biol., 1, 31-33.
6. Del Priore, V., Snay, C.A., Bahr, A. and Cole, C.N. (1996) The product of the Saccharomyces cerevisiae RSSI gene, identified as a high-copy suppressor of the rat7-1 temperature-sensitive allele of the RAT7/ NUP159 nucleoporin, is required for efficient mRNA export. Mol. Biol. Cell, 7, 1601-1621.
7. Del Priore, V., Heath, C.V., Snay, C.A., MacMillan, A., Gorsch, L.C., Dagher, S. and Cole, C.N. (1997) A structure/function analysis of Rat7p/Nup159p, an essential nucleoporin of Saccharomyces cerevisiae. J. Cell Sci., 110, 2897-2999.
8. Doye, V. and Hurt, E. (1997) From nucleoporins to nuclear pore complexes. Curr. Opin. Cell Biol., 9, 401-411.
9. Fabre, E. and Hurt, E. (1997) Yeast genetics to dissect the nuclear pore complex and nucleocytoplasmic trafficking. Annu. Rev. Genet., 31, 277-313.
10. Fahrenkrog, B., Hurt, E.C., Aebi, U. and Panté, N. (1998) Molecular architecture of the yeast nuclear pore complex: localization of Nsp1p subcomplexes. J. Cell Biol., 143, 577-588.
11. Farjot, G., Sergeant, A. and Mikaélian, I. (1999) A new nucleoporin-like protein interacts with both HIV-1 Rev nuclear export signal and CRM-1. J. Biol. Chem., 274, 17309-17317.
12. Floer, M. and Blobel, G. (1999) Putative reaction intermediates in Crm1-mediated nuclear protein export. J. Biol. Chem., 274, 16279-16286.
13. Fontoura, B.M.A., Blobel, G. and Matunis, M.J. (1999) A conserved biogenesis pathway for nucleoporins: proteolytic processing of a 186-kilodalton precursor generates Nup98 and the novel nucleoporin, Nup96. J. Cell Biol., 144, 1097-1112.
14. Fritz, C.C., Zapp, M.L. and Green, M.R. (1995) A human nucleoporin-like protein that specifically interacts with HIV Rev. Nature, 376, 530-533.
15. Görlich, D. (1998) Transport into and out of the cell nucleus. EMBO J., 17, 2721-2727.
16. Grüter, P., Tabernero, C., von Kobbe, C., Schmitt, C., Saavedra, C., Bachi, A., Wilm, M., Felber, B.K. and Izaurralde, E. (1998) TAP, the human homolog of Mex67p, mediates CTE-dependent RNA export from the nucleus. Mol. Cell, 1, 649-659.
17. Hodge, C.A., Colot, H.V., Stafford, P. and Cole, C.N. (1999) Rat8p/Dbp5 is a shuttling transport factor that interacts with Rat7p/Nup159p and Gle1p and suppresses the mRNA export defect of xpo1-1 cells. EMBO J., 18, 5778-5788.
18. Holm, C. (1993) A functional approach to identifying yeast homologs of genes from other species. Methods, 5, 102-109.
19. Hurwitz, M.E., Strambio-de-Castillia, C. and Blobel, G. (1998) Two yeast nuclear pore complex proteins involved in mRNA export form a cytoplasmically oriented subcomplex. Proc. Natl Acad. Sci. USA, 95, 11241-11245.
20. Izaurralde, E. and Adam, S. (1998) Transport of macromolecules between the nucleus and the cytoplasm. RNA, 4, 351-364.
21. Izaurralde, E., Kutay, U., von Kobbe, C., Mattaj, I.W. and Gorlich, D. (1997) The asymmetric distribution of the constituents of the Ran system is essential for transport into and out of the nucleus. EMBO J., 16, 6535-6547.
22. Katahira, J., Strässer, K., Podtelejnikov, A., Mann, M., Jung, J.U. and Hurt, E. (1999) The Mex67p-mediated nuclear mRNA export pathway is conserved from yeast to human. EMBO J., 18, 2593-2609.
23. Kraemer, D.M., Strambio-de-Castillia, C., Blobel, G. and Rout, M.P. (1995) The essential yeast nucleoporin NUP159 is located on the cytoplasmic side of the nuclear pore complex and serves in karyopherin-mediated binding of transport substrate. J. Biol. Chem., 270, 19017-19021.
24. Liao, X.C., Tang, J. and Rosbash, M. (1993) An enhancer screen identifies a gene that encodes the yeast U1 snRNP A protein: implications for snRNP protein function in pre-mRNA splicing. Genes Dev., 7, 419-428.
25. Longtine, M.S., McKenzie, A., Demarini, D.J., Shah, N.G., Wach, A., Brachat, A., Philippsen, P. and Pringle, J.R. (1998) Additional modules for versatile and economical PCR-based gene deletion and modification in Saccharomyces cerevisiae. Yeast, 14, 953-961.
26. Mattaj, I.W. and Englmeier, L. (1998) Nucleocytoplasmic transport: the soluble phase. Annu. Rev. Biochem., 67, 265-306.
27. Murphy, R. and Wente, S.R. (1996) An RNA-export mediator with an essential nuclear export signal. Nature, 383, 357-360.
28. Nakielny, S., Fischer, U., Michael, W.M. and Dreyfuss, G. (1997) RNA transport. Annu. Rev. Neurosci., 20, 269-301.
29. Nakielny, S., Shaikh, S., Burke, B. and Dreyfuss, G. (1999) Nup153 is an M9-containing mobile nucleoporin with a novel Ran-binding domain. EMBO J., 18, 1982-1995.
30. Neville, M. and Rosbash, M. (1999) The NES-Crm1p export pathway is not a major mRNA export route in Saccharomyces cerevisiae. EMBO J., 18, 3746-3756.
31. Neville, M., Stutz, F., Lee, L., Davis, L.I. and Rosbash, M. (1997) Evidence that the importin-beta family member Crm1p bridges the interaction between Rev and the nuclear pore complex during nuclear export in S.cerevisiae. Curr. Biol., 7, 767-775.
32. Noble, S.M. and Guthrie, C. (1996) Identification of novel genes required for yeast pre-mRNA splicing by means of cold-sensitive mutations. Genetics, 143, 67-80.
33. Pollard, V.W. and Malim, M.H. (1998) The HIV-1 Rev protein. Annu. Rev. Microbiol., 52, 491-532.
34. + mRNA. Genes Dev., 11, 2845-2856.
35. Samos-Rosa, H., Moreno, H., Simos, G., Segref, A., Fahrenkrog, B., Panté, N. and Hurt, E. (1998) Nuclear mRNA export requires complex formation between Mex67p and Mtr2p at the nuclear pores. Mol. Cell. Biol., 18, 6826-6838.
36. Schmitt, C. et al. (1999) Dbp5p, a DEAD box-protein required for mRNA export, is recruited to the cytolasmic fibrils of nuclear pore complex via a conserved interaction with CAN/Nup159p. EMBO J., 18, 4332-4347.
37. Siniossoglou, S., Wimmer, C., Rieger, M., Doye, V., Tekotte, H., Weise, C., Emig, S., Segref, A. and Hurt, E.C. (1996) A novel complex of nucleoporins, which includes Sec13p and a Sec13p homolog, is essential for normal nuclear pores. Cell, 84, 265-275.
38. Snay-Hodge, C.A., Colot, H.V., Goldstein, A.L. and Cole, C.N. (1998) Dbp5p/Rat8p is a yeast nuclear pore-associated DEAD-box protein essential for RNA export. EMBO J., 17, 2663-2676.
39. Stade, K., Ford, C.S., Guthrie, C. and Weis, K. (1997) Exportin 1 (Crm1p) is an essential nuclear export factor. Cell, 90, 1041-1050.
40. Stoffler, D., Fahrenkrog, B. and Aebi, U. (1999) The nuclear pore complex: from molecular architecture to functional dynamics. Curr. Opin. Cell Biol., 11, 391-401.
41. Stutz, F. and Rosbash, M. (1998) Nuclear RNA export. Genes Dev., 12, 3303-3319.
42. Stutz, F., Neville, M. and Rosbash, M. (1995) Identification of a novel nuclear pore-associated protein as a functional target of the HIV-1 Rev protein in yeast. Cell, 82, 495-506.
43. Stutz, F., Kantor, J., Zhang, D., McCarthy, T., Neville, M. and Rosbash, M. (1997) The yeast nucleoporin Rip1p contributes to multiple export pathways with no essential role for its FG-repeat region. Genes Dev., 11, 2857-2868.
44. Teixeira, M.T., Siniossoglou, S., Podtelejnikov, S., Bénichou, J.C., Mann, M., Dujon, B., Hurt, E. and Fabre, E. (1997) Two functionally distinct domains generated by in vivo cleavage of Nup145p: a novel biogenesis pathway for nucleoporins. EMBO J., 16, 5086-5097.
45. + RNA export. EMBO J., 17, 2651-2662.
46. Van Laer, L. et al. (1997) Refined mapping of a gene for autosomal dominant progressive sensorineural hearing loss (DFNA5) to a 2-cM region, and exclusion of a candidate gene that is expressed in the cochlea. Eur. J. Hum. Genet., 5, 397-405.
47. + RNA export. Proc. Natl Acad. Sci. USA, 95, 6779-6784.
48. Zolotukhin, A.S. and Felber, B.K. (1999) Nucleoporins Nup98 and Nup214 participate in nuclear export of human immunodeficiency virus type 1 Rev. J. Virol., 73, 120-127.