The NES-Crm1p export pathway is not a major mRNA export route in Saccharomyces cerevisiae

EMBO Journal

Volumn 18, Issue 13, 1999, Pages 3746-3756

  Neville, Megan ^a   Rosbash, Michael ^a  

^a BRANDEIS UNIVERSITY   (United States)

Author keywords

CRM1; Leptomycin B; mRNA export; Nuclear export signal; Saccharomyces cerevisiae

Indexed keywords

FUNGAL RNA; LEPTOMYCIN B; MESSENGER RNA; POLYADENYLATED RNA;

AMINO ACID SUBSTITUTION; ARTICLE; IN SITU HYBRIDIZATION; NONHUMAN; PRIORITY JOURNAL; SACCHAROMYCES CEREVISIAE; SCHIZOSACCHAROMYCES POMBE;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; BINDING SITES; BIOLOGICAL TRANSPORT; CARRIER PROTEINS; CELL NUCLEUS; CONSERVED SEQUENCE; FATTY ACIDS, UNSATURATED; GENES, FUNGAL; GTP-BINDING PROTEINS; GTPASE-ACTIVATING PROTEINS; HEAT-SHOCK PROTEINS; KARYOPHERINS; MICROBIAL SENSITIVITY TESTS; MOLECULAR SEQUENCE DATA; POLY A; PROTEIN BIOSYNTHESIS; PROTEIN SORTING SIGNALS; RECEPTORS, CYTOPLASMIC AND NUCLEAR; RECOMBINANT FUSION PROTEINS; RNA, MESSENGER; SACCHAROMYCES CEREVISIAE; SCHIZOSACCHAROMYCES; SCHIZOSACCHAROMYCES POMBE PROTEINS; SPECIES SPECIFICITY;

NES; SACCHAROMYCES CEREVISIAE; SCHIZOSACCHAROMYCES POMBE; VERTEBRATA;

EID: 0033168194     PISSN: 02614189     EISSN: None     Source Type: Journal    
DOI: 10.1093/emboj/18.13.3746     Document Type: Article

References (41)

1. Amberg, D.C. Goldstein, A.L. and Cole, C.N. (1989) Isolation and characterization of RAT1: an essential gene of Saccharomyces cerevisiae required for the efficient nucleocytoplasmic trafficking of mRNA. Genes Dev., 6, 1173-1189
2. Askjaer, P., Jensen, T.H., Nilsson, J., Englmeier, L. and Kjems, J. (1998) The specificity of the CRM1-Rev nuclear export signal interaction is mediated by RanGTP. J. Biol. Chem., 273, 33414-33422.
3. Bergmann, E.D., Ginsburg, D. and Pappo, R. (1959) The Michael Reaction. Organic Reactions, 10, 179-555.
4. Bogerd, H.P., Echarri, A., Ross, T.M. and Cullen, B.R. (1998) Inhibition of human immunodeficiency virus Rev and human T-cell leukemia virus Rex function, but not Mason-Pfizer monkey virus constitutive transport element activity, by a mutant human nucleoporin targeted to Crm1. J. Virol., 72, 8627-8635.
5. Engel, K., Kotlyarov, A. and Gaestel, M. (1998) Leptomycin B-sensitive nuclear export of MAPKAP kinase 2 is regulated by phosphorylation. EMBO J., 17, 3363-3371.
6. Feng, W., Benko, A.L., Lee, J.-H., Stanford, D.R. and Hopper, A.K. (1999) Antagonistic effects of NES and NLS motifs determine S. cerevisiae Rna1p subcellular distribution. J. Cell Sci., 112, 339-347.
7. Fischer, U., Huber, J., Boelens, W.C., Mattaj, I.W. and Luhrmann, R. (1995) The HIV-1 Rev activation domain is a nuclear export signal that accesses an export pathway used by specific cellular RNAs. Cell, 82, 475-483.
8. Fornerod, M., Ohno, M., Yoshida, M. and Mattaj, I.W. (1997a) CRM1 is an export receptor for leucine-rich nuclear export signals. Cell, 90, 1051-1060.
9. Fornerod, M., van Deursen, J., van Baal, S., Reynolds, A., Davis, D., Murti, K.G., Fransen, J. and Grosveld, G. (1997b) The human homologue of yeast CRM1 is in a dynamic subcomplex with CAN/ Nup214 and a novel nuclear pore component Nup88. EMBO J., 16, 807-816.
10. Forrester, W., Stutz, F., Rosbash, M. and Wickens, M. (1992) Defects in mRNA 3′-end formation, transcription initiation and mRNA transport associated with the yeast mutation prp20: possible coupling of mRNA processing and chromatin structure. Genes Dev., 6, 1914-1926.
11. Fukuda, M., Asano, S., Nakamura, T., Adachi, M., Yoshida, M., Yanagida, M. and Nishida, E. (1997a) CRM1 is reponsible for intracellular transport mediated by the nuclear export signal. Nature, 390, 308-311.
12. Fukuda, M., Gotoh, Y. and Nishida, E. (1997b) Interaction of MAP kinase with MAP kinase kinase: its possible role in the control of nucleocytoplasmic transport of MAP kinase. EMBO J., 16, 1901-1908.
13. Görlich, D. (1998) Transport into and out of the cell nucleus. EMBO J., 17, 2721-2727.
14. Görlich, D., Dabrowski, M., Bischoff, F.R., Kutay, U., Bork, P., Hartmann, E., Prehn, S. and Izaurralde, E. (1997) A novel class of RanGTP binding proteins. J. Cell Biol., 138, 65-80.
15. Hamamoto, T., Gunji, S., Tsuji, H. and Beppu, T. (1983) Leptomycin A and B, new antifungal antibiotics. I. Taxonomy of the producing strain and their fermentation, purification and characterization. J. Antibiot. (Tokyo), 36, 639-645.
16. Hamamoto, T., Uozumi, T. and Beppu, T. (1985) Leptomycin A and B, new antifungal antibiotics. III. Mode of action of leptomycin B on Schizosaccharomyces pombe. J. Antibiot. (Tokyo), 38, 1573-1580.
17. Izaurralde, E., Kutay, U., von Kobbe, C., Mattaj, I.W. and Görlich, D. (1997) The asymmetric distribution of the constituents of the Ran system is essential for transport into and out of the nucleus. EMBO J., 16, 6535-6547.
18. Jakel, S. and Görlich, D. (1998) Importin β, transportin, RanBP5 and RanBP7 mediate nuclear import of ribosomal proteins in mammalian cells. EMBO J., 17, 4491-4502.
19. Kudo, N., Wolff, B., Sekimoto, T., Schreiner, E., Yoneda, Y., Yanagida, M., Horinouchi. S. and Yoshida, M. (1998) Leptomycin B inhibition of signal-mediated nuclear export by direct binding to CRM1. Exp. Cell Res., 242, 540-547.
20. Lindquist, S. and Craig, E.A. (1988) The heat-shock proteins. Annu. Rev. Genet., 22, 631-677.
21. Mattaj, I.W. and Englmeier, L. (1998) Nucleocytoplasmic transport: the soluble phase. Annu. Rev. Biochem., 67, 265-306.
22. Neville, M., Lee, L., Stutz, F., Davis, L.I. and Rosbash, M. (1997) Evidence that the importin-beta family member Crm1p bridges the interaction between Rev and the nuclear pore complex during nuclear export in S. cerevisiae. Curr. Biol., 7, 767-775.
23. Nishi, K., Yoshida, M., Fujiwara, D., Nishikawa, M., Horinouchi, S. and Beppu, T. (1994) Leptomycin B targets a regulatory cascade of crm1, a fission yeast nuclear protein, involved in control of higher order chromosome structure and gene expression. J. Biol. Chem., 269, 6320-6324.
24. Nonet, M., Scafe, C., Sexton, J. and Young, R. (1987) Eucaryotic RNA polymerase conditional mutant that rapidly ceases mRNA synthesis. Mol. Cell. Biol., 7, 1602-1611.
25. Ossareh-Nazari, B., Bachelerie, F. and Dargemont, C. (1997) Evidence for a role of CRM1 in signal-mediated nuclear protein export. Science, 278, 141-144.
26. Otero, G.C., Harris, M.E., Donello, J.E. and Hope, T.J. (1998) Leptomycin B inhibits equine infectious anemia virus Rev and feline immunodeficiency virus rev function but not the function of the hepatitus B virus posttranscriptional regulatory element. J. Virol., 72, 7593-7597.
27. Pollard, V.W. and Malim, M.H. (1998) The HIV-1 REV protein. Annu. Rev. Microbiol., 52, 491-532.
28. Saavedra, C., Tung, K.-S., Amberg, D.C., Hopper, A.K. and Cole, C.N. (1996) Regulation of mRNA export in response to stress in Saccharomyces cerevisiae. Genes Dev., 10, 1608-1620.
29. Saavedra, C.A., Hammell, C.M., Heath, C.V. and Cole, C.N. (1997) Yeast heat shock mRNAs are exported through a distinct pathway defined by Rip1p. Genes Dev., 11, 2845-2856.
30. Seedorf, M. and Silver, P.A. (1997) Importin/karyopherin protein family members required for mRNA export from the nucleus. Proc. Natl Acad. Sci. USA, 94, 8590-8595.
31. Stade, K., Ford, C.S., Guthrie, C. and Weis, K. (1997) Exportin 1 (Crm1p) is an essential nuclear export factor. Cell, 90, 1041-1050.
32. Stutz F. and Rosbashm M. (1998) Nuclear RNA export. Genes Dev., 12, 3303-3319.
33. Stutz, F., Kantor, J., Zhang, D., McCarthy, T., Neville, M. and Rosbash, M. (1997) The yeast nucleoporin Rip1p contributes to multiple export pathways with no essential role for its FG-repeat region. Genes Dev., 11, 2857-2868.
34. Taagepera, S., McDonald, D., Loeb, J., Whitaker, L., McElroy, A., Wang, J.J. and Hope, T.J. (1999) Nuclear-cytoplasmic shuttling of C-ABL tyrosine kinase. Proc. Natl Acad. Sci. USA, 95, 7457-7462.
35. Taura, T., Krebber, H. and Silver, P.A. (1998) A member of the Ran-binding protein family, Yrb2p, is involved in nuclear protein export. Proc. Natl Acad. Sci. USA, 95, 7427-7432.
36. Toone, W.M., Kuge, S., Samuels, M., Morgan, B.A., Toda, T. and Jones, N. (1998) Regulation of the fission yeast transcription factor Pap1 by oxidative stress: requirement for the nuclear export factor Crm1 (Exportin) and the stress-activated MAP kinase Sty1/Spc1. Genes Dev., 12, 1453-1463.
37. Wada, A., Fukuda, M., Mishima, M. and Nishida, E. (1998) Nuclear export of actin: a novel mechanism regulating the subcellular localization of a major cytoskeletal protein. EMBO J., 17, 1635-1641.
38. Wen, W., Meinkoth, J.L., Tsien, R.Y. and Taylor, S.S. (1995) Identification of a signal for rapid export of proteins from the nucleus. Cell, 82, 463-473.
39. Wolff, B., Sanglier, J.-J. and Wang, Y. (1997) Leptomycin B is an inhibitor of nuclear export: inhibition of nucleo-cytoplasmic translocation of the human immunodeficiency virus type 1 (HIV-1) Rev protein and Rev-dependent mRNA. Chem. Biol., 4, 139-147.
40. Yan, C., Lee, L.H. and Davis, L.I. (1998) Crm1p mediates regulated nuclear export of a yeast AP-1 like transcription factor. EMBO J., 17, 7416-7429.
41. Yoshida, M., Nishikawa. M., Nishi, K., Abe, K., Horinouchi, S. and Beppu, T. (1990) Effects of leptomycin B on the cell cycle of fibroblasts and fission yeast cells. Exp. Cell Res., 187, 150-156.