Separate binding sites on nuclear transport factor 2 (NTF2) for GDP-Ran and the phenylalanine-rich repeat regions of nucleoporins p62 and Nsp1p

Journal of Molecular Biology

Volumn 263, Issue 4, 1996, Pages 517-524

  Clarkson, W David ^a   Kent, Helen M ^a   Stewart, Murray ^a  

^a MRC LABORATORY OF MOLECULAR BIOLOGY   (United Kingdom)

Author keywords

NTF2; Nuclear import; Nucleoporin p62; Ran; Transport

Indexed keywords

GUANOSINE DIPHOSPHATE; NUCLEOPORIN;

ARTICLE; BINDING SITE; CYTOPLASM; LIVER HOMOGENATE; NUCLEIC ACID SYNTHESIS; PRIORITY JOURNAL;

VERTEBRATA;

EID: 0030575845     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.1996.0594     Document Type: Editorial

References (40)

1. Bullock, T. L., Clarkson, W. D., Kent, H. M. & Stewart, M. (1996). 1.6 Å structure of nuclear transport factor 2 (NTF2). J. Mol. Biol. 260, 422-431.
2. Buss, F. & Stewart, M. (1995). Macromolecular interactions in the nucleoporin p62 complex of rat nuclear pores: binding of nucleoporin p54 to the rod domain of p62. J. Cell Biol. 128, 251-261.
3. Buss, F., Kent, H., Stewart, M., Bailer, S. M. & Hanover, J. A. (1994). Role of different domains in the self-association of rat nucleoporin p62. J. Cell Sci. 107, 631-638.
4. Carmo-Fonseca, M., Kern, H. & Hurt, E. C. (1991). Human nucleoporin p62 and the essential yeast nuclear pore protein NSP1 show sequence homology and a similar domain organisation. Eur. J. Cell Biol. 55, 17-30.
5. Cordes, V., Waizenegger, I. & Krohne, G. (1991). Nuclear pore complex glycoprotein p62 of Xenopus laevis and mouse: cDNA cloning and identification of its glycosylated region. Eur. J. Cell Biol. 55, 31-47.
6. Feldherr, C. M., Kallenbach, E. & Schultz, N. (1984). Movement of a karyophilic protein through the nuclear pores of oocytes. J. Cell Biol. 99, 2216-2222.
7. Finlay, D. R., Meier, E., Bradley, P., Horecka, J. & Forbes, D. J. (1991). A complex of nuclear pore proteins required for pore function. J. Cell Biol. 114, 169-183.
8. Görlich, D. & Mattaj, I. W. (1996). Nucleocytoplasmic transport. Science 271, 1513-1518.
9. Görlich, D., Prehn, S., Lasker, R. A. & Hartmann, E. (1994). Isolation of a protein that is essential for the first step nuclear protein import. Cell, 79, 767-778.
10. Görlich, D., Vogel, F., Mills, A. D., Hartmann, E. & Laskey, R. A. (1995). Distinct functions for the two importin subunits in nuclear protein import. Nature, 377, 246-248.
11. Grandi, P., Doyle, V. & Hurt, E. C. (1993). Purification of NSP1 reveals complex formation with 'GLFG' nucleoporins and a novel nuclear pore protein NIC96. EMBO J. 12, 3061-3071.
12. Grandi, P., Schlaich, N., Tekotte, H. & Hurt, E. C. (1995). Functional interaction of Nic96p with a core nucleoporin complex consisting of Nsp1p, Nup49p and a novel protein Nup57p. EMBO J. 14, 76-87.
13. Guan, T., Müller, S., Klier, G., Panté, N., Blevitt, J. M., Haner, M., Paschal, B., Aebi, U. & Gerace, L. (1995). Structural analysis of the p62 complex, an assembly of O-linked glycoproteins that localises near the central gated channel of the nuclear complex. Mol. Biol. Cell, 6, 1591-1603.
14. Harlow, E. & Lane, D. (1988). Antibodies: A Laboratory Manual, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
15. Kent, H. M., Clarkson, W. D., Bullock, T. L. & Stewart, M. (1996). Crystallisation and preliminary X-ray diffraction analysis of nuclear transport factor (NFT2). J. Struct. Biol. 116, 325-328.
16. Khyse-Anderson, J. (1984). Electroblotting of multiple gels: a simple apparatus without buffer tank for rapid transfer of proteins from polyacrylamide to nitrocellulose. J. Biochem. Biophys. Methods, 10, 203-209.
17. Lewin, B. (1994). Genes V, Oxford University Press, Oxford, UK.
18. Lubas, W. A., Smith, M., Starr, C. M. & Hanover, J. A. (1995). Analysis of nuclear pore protein p62 glycosylation. Biochemistry, 34, 1686-1694.
19. Matsudiara, P. & Burgess, D. R. (1978). SDS microslab linear gradient PAGE. Anal. Biochem. 87, 386-396.
20. Melchior, F., Paschal, B., Evans, J. & Gerace, L. (1993). Inhibition of nuclear protein import by nonhydrolysable analogues of GTP and identification of the small GTPase Ran/TC4 as an essential transport factor. J. Cell Biol. 123, 1649-1659.
21. Moore, M. S., & Blobel, G. (1993). The GTP-binding protein Ran/TC4 is required for protein import into the nucleus. Nature, 365, 661-663.
22. Moore, M. S. & Blobel, G. (1994). Purification of a Ran-interacting protein that is required for protein import into the nucleus. Proc. Natl Acad. Sci. USA, 91, 10212-10216.
23. 2 subunit binds nuclear localisation signal and β subunit interacts with peptide repeat-containing nucleoporins. Proc. Natl Acad. Sci. USA, 92, 6532-6536.
24. Nehrbass, U. & Blobel, G. (1996). Role of nuclear transport factor p10 in nuclear import. Science, 272, 120-122.
25. Nehrbass, U., Kern, H., Mutvei, A., Horstmann, H., Marshallsay, B. & Hurt, E. C. (1990). NSP1: a yeast nuclear envelope protein localised at the nuclear pores exerts its essential function by its carboxy-terminal domain. Cell, 61, 979-989.
26. Newmeyer, D. D. & Forbes, D. J. (1988). Nuclear import can be separated indistinct steps in vitro: nuclear pore binding and translocation. Cell, 52, 641-653.
27. Paschal, B. M. & Gerace, L. (1995). Identification of NTF2, a cytosolic factor for nuclear import that interacts with nuclear pore complex protein p62. J. Cell Biol. 129, 925-937.
28. Paschal, B. M., Delphin, C. & Gerace, L. (1996). Nucleotide-specific interaction of Ran/TC4 with nuclear transport factors NTF2 and p97. Proc. Natl Acad. Sci. USA, 93, 7679-7683.
29. Radu, A., Blobel, G. & Moore, M. S. (1995). Identification of a protein complex that is required for nuclear protein import and mediates docking of import substrate to distinct nucleoporins. Proc. Natl Acad. Sci. USA, 92, 1769-1773.
30. Rexach, M. & Blobel, G. (1995). Protein import into nuclei: associated and dissociation reactions involving transport substrate, transport factors, and nucleoporins. Cell, 83, 683-692.
31. Richardson, W. D., Mills, A. D., Dilworth, S. M., Laskey, R. A. & Dingwall, C. (1988). Nuclear protein migration involves two steps: rapid binding at the nuclear envelope followed by slower translocation through the nuclear pores. Cell, 52, 655-664.
32. Rout, M. P. & Wente, S. R. (1994). Pores for thought: nuclear pore complex proteins. Trends Cell Biol. 4, 357-365.
33. Simos, G. & Hurt, E. C. (1995). Nucleocytoplasmic transport: factors and mechanisms. FEBS Letters, 369, 107-112.
34. Starr, C. M., D'Onofrio, M., Park, M. K. & Hanover, J. A. (1990). Primary sequence and heterologous expression of nuclear pore glycoprotein p62. J. Cell Biol. 110, 1861-1871.
35. Stewart, M. & Clarkson, W. D. (1996). Nuclear pores and macromolecular assemblies involved with nucleocytoplasmic transport. Curr. Opin. Struct. Biol. 6, 162-165.
36. Stewart, M., Whytock, S. & Mills, A. D. (1990). Association of gold-labelled nucleoplasmin with the centres of ring components of Xenopus oocyte nuclear pore complexes. J. Mol. Biol. 213, 575-582.
37. Sweet, D. J. & Gerace, L. (1995). Taking from the cytoplasm and giving to the pore: soluble transport factors in nuclear protein import. Trends Cell Biol. 5, 444-447.
38. Way, M., Pope, B., Gooch, J., Hawkins, M. & Weeds, A. G. (1990). Identification of a region in segment 1 of gelsolin critical for actin binding. EMBO J. 9, 4103-4109.
39. Wu, J., Matunis, M. J., Kraemer, D., Blobel, G. & Coutavas, E. (1995). Nup358, a cytoplasmically exposed nucleoporin with peptide repeats, Ran-GTP binding sites, zinc fingers, a cyclophilin A homologous domain, and a leucine-rich region. J. Biol. Chem. 270, 14209-14213.
40. Yokoyama, N., Hayashi, N., Seki, T., Panré, N., Ohba, T., Nishii, K., Kuma, K., Hayashida, T., Miyata, T., Aebi, U., Fukui, M. & Nishimoto, T. (1995). A giant nucleopore protein that binds Ran/TC4. Nature, 376, 184-188.