New developments in isotope labeling strategies for protein solution NMR spectroscopy

Current Opinion in Structural Biology

Volumn 10, Issue 5, 2000, Pages 585-592

  Goto, Natalie K ^a,b   Kay, Lewis E ^a,c  

^a UNIVERSITY OF TORONTO   (Canada)

^b HOSPITAL FOR SICK CHILDREN   (Canada)

^c UNIVERSITY OF TORONTO   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

CHEMICAL STRUCTURE; CHO CELL; ISOTOPE LABELING; NONHUMAN; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; NUCLEAR OVERHAUSER EFFECT; PICHIA PASTORIS; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN STRUCTURE; PROTON TRANSPORT; REVIEW;

BACTERIA (MICROORGANISMS); PICHIA PASTORIS;

EID: 0033794450     PISSN: 0959440X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0959-440X(00)00135-4     Document Type: Review

References (74)

1. Multidimensional nuclear-magnetic-resonance methods for protein studies
2. Structures of larger proteins in solution: Three- and four-dimensional heteronuclear NMR spectroscopy
3. 15N multidimensional NMR to study the structure and dynamics of proteins
4. 15N-enriched proteins - Application to triple-resonance 4D J-connectivity of sequential amides
5. 2H-labeled proteins with high-sensitivity
6. NMR sequential assignment of Escherichia coli thioredoxin utilizing random fractional deuteriation
7. NMR signal assignments of amide protons in the alpha-helical domains of staphylococcal nuclease
8. Solution structure of an rRNA methyltransferase (ErmAM) that confers macrolide-lincosamide-streptogramin antibiotic resistance
9. Solution structure of the 30 kDa N-terminal domain of enzyme I of the Escherichia coli phosphoenolpyruvate:sugar phosphotransferase system by multidimensional NMR
10. Structure of Cdc42 in complex with the GTPase-binding domain of the 'Wiskott-Aldrich syndrome' protein
11. TROSY in triple-resonance experiments: New perspectives for sequential NMR assignment of large proteins
12. 2H-decoupling
13. Solution NMR studies of a 42 kDa Escherichia coli maltose binding protein beta-cyclodextrin complex: Chemical shift assignments and analysis
14. 13C-labeled proteins
15. 2 relaxation by mutual cancellation of dipole-dipole coupling and chemical shift anisotropy indicates an avenue to NMR structures of very large biological macromolecules in solution
16. Selective methyl group protonation of perdeuterated proteins
17. Surface, subunit interfaces and interior of oligomeric proteins
18. 3 spin systems via polarization-transfer techniques
19. 2H-labeled proteins
20. 1H-δ1 methyl) isoleucine into proteins for multidimensional NMR studies
21. Global folds of proteins with sparse numbers of NOEs using residual dipolar couplings: Application to the 370 residue maltodetrin binding protein
22. Structure of the cell-adhesion fragment of intimin from enteropathogenic Escherichia coli
23. The Ig fold of the core binding factor alpha Runt domain is a member of a family of structurally and functionally related Ig-fold DNA-binding domains
24. NMR structure and mutagenesis of the N-terminal Dbl homology domain of the nucleotide exchange factor Trio
25. Structure and mutagenesis of the Dbl homology domain
26. Characterization of long-range structure in the denatured state of staphylococcal nuclease. II. Distance restraints from paramagnetic relaxation and calculation of an ensemble of structures
27. Utilization of site-directed spin labeling and high-resolution heteronuclear nuclear magnetic resonance for global fold determination of large proteins with limited nuclear Overhauser effect data
28. Protein global fold determination using site-directed spin and isotope labeling
29. 13C' dipolar couplings in a perdeuterated 30 kDa protein dissolved in a dilute liquid crystalline phase
30. TROSY-based HNCO pulse sequences for the measurement of (1)HN-(15)N, (15)N-(13)CO, (1)HN-(13)CO, (13)CO-(13)Cα and (1)HN-(13)Cα dipolar couplings in (15)N, (13)C, (2)H-labeled proteins
31. Measurement of (3h)J(NC') connectivities across hydrogen bonds in a 30 kDa protein
32. Protein structure determination using molecular fragment replacement and NMR dipolar couplings
33. 2D and 3D NMR-study of phenylalanine residues in proteins by reverse isotopic labeling
34. 15N-labeled background to a 47 kDa homodimer: Potential for NMR structure determination of large proteins
35. A method for assigning phenylalanines in proteins
36. 1H]-ZQ-TROSY NMR experiment with diagonal peak suppression
37. Pilus chaperone FimC-adhesin FimH interactions mapped by TROSY-NMR
38. TROSY triple-resonance four-dimensional NMR spectroscopy of a 46 ns tumbling protein
39. 2H labeled proteins
40. Protein splicing of inteins and hedgehog autoproteolysis: Structure, function, and evolution
41. Segmental isotope labeling for protein NMR using peptide splicing
42. Improved segmental isotope labeling of proteins and application to a larger protein
43. NMR observation of selected segments in a larger protein: Central-segment isotope labeling through intein-mediated ligation
44. Chemical ligation of folded recombinant proteins: Segmental isotopic labeling of domains for NMR studies
45. Chemical protein synthesis
46. 13C NMR relaxation analysis
47. 2H-labeled proteins in solution
48. 13C cross-correlation NMR experiments: Application to the N-terminal SH3 domain from drk
49. Correlation between binding and dynamics at SH2 domain interfaces
50. Redistribution and loss of side chain entropy upon formation of a calmodulin-peptide complex
51. Backbone and methyl dynamics of the regulatory domain of troponin C: Anisotropic rotational diffusion and contribution of conformational entropy to calcium affinity
52. Changes in side-chain and backbone dynamics identify determinants of specificity in RNA recognition by human U1A protein
53. 13C NMR relaxation techniques for the study of protein methyl group dynamics in solution
54. Effect of hydrophobic core packing on sidechain dynamics
55. 13C relaxation of CHD methyl isotopmers to detect slow conformational changes of protein sidechains
56. 13C relaxation measurements of methyl groups in proteins
57. Heterologous protein expression in the methylotrophic yeast Pichia pastoris
58. Production of large quantities of isotopically labeled protein in Pichia pastoris by fermentation
59. Solution structure of an EGF module pair from the Plesmodium falciparum merozoite surface protein 1
60. Use of Pichia pastoris for the expression, purification, and characterization of rat calretinin 'EF-hand' domains
61. Isotopically labeled bovine β-lactoglobulin for NMR studies expressed in Pichia pastoris
62. High-level secretion and very efficient isotopic labeling of tick anticoagulant peptide (Tap) expressed in the methylotrophic yeast, Pichia pastoris
63. NMR analysis of the N-terminal SRCR domain of human CD5: Engineering of a glycoprotein for superior characteristics in NMR experiments
64. 15N-labeled hen lysozyme in Pichia pastoris and identification of the site in hen lysozyme where phosphate ion binds using NMR measurements
65. The solution structure of type II antifreeze protein reveals a new member of the lectin family
66. Heterologous expression of a deuterated membrane-integrated receptor and partial deuteration in methylotrophic yeasts
67. 1H resonance assignments and secondary structure of the 13.6 kDa glycosylated adhesion domain of human CD2
68. A practical method for uniform isotopic labeling of recombinant proteins in mammalian cells
69. Expression of human chorionic gonadotropin uniformly labeled with NMR isotopes in Chinese hamster ovary cells: An advance toward rapid determination of glycoprotein structures
70. Improved resolution and sensitivity of triple-resonance NMR methods for the structural analysis of proteins by use of a backbone-labeling strategy
71. Cell-free production and stable-isotope labeling of milligram quantities of proteins
72. A highly efficient and robust cell-free protein synthesis system prepared from wheat embryos: Plants apparently contain a suicide system directed at ribosomes
73. Dual amino acid-selective and site-directed stable-isotope labeling of the human c-Ha-Ras protein by cell-free synthesis
74. tRNA-mediated protein engineering