A robust and cost-effective method for the production of Val, Leu, Ile (δ1) methyl-protonated 15N-, 13C-, 2H-labeled proteins

Journal of Biomolecular NMR

Volumn 13, Issue 4, 1999, Pages 369-374

  Goto, Natalie K ^a,b   Gardner, Kevin H ^c,d   Mueller, Geoffrey A ^b,c   Willis, Randall C ^b   Kay, Lewis E ^c  

^a UNIVERSITY OF TORONTO   (Canada)

^b HOSPITAL FOR SICK CHILDREN   (Canada)

^c UNIVERSITY OF TORONTO   (Canada)

^d UNIVERSITY OF TEXAS AT DALLAS   (United States)

Author keywords

ketobutyrate; ketoisovalerate; Deuteration; Methyl protonation

Indexed keywords

2 OXOBUTYRIC ACID; 2 OXOISOVALERIC ACID; CARBON 13; DEUTERIUM; ISOLEUCINE; LEUCINE; NITROGEN 15; VALINE;

AMINO ACID METABOLISM; AMINO ACID SYNTHESIS; ARTICLE; COST EFFECTIVENESS ANALYSIS; CULTURE MEDIUM; ESCHERICHIA COLI; ISOTOPE LABELING; MACROMOLECULE; NONHUMAN; NUCLEAR MAGNETIC RESONANCE; NUCLEAR OVERHAUSER EFFECT; PRIORITY JOURNAL; PROTEIN EXPRESSION; STRUCTURE ANALYSIS;

CARBON ISOTOPES; DEUTERIUM; INDICATORS AND REAGENTS; ISOLEUCINE; ISOTOPE LABELING; LEUCINE; NITROGEN ISOTOPES; NUCLEAR MAGNETIC RESONANCE, BIOMOLECULAR; PROTEIN CONFORMATION; PROTEINS; VALINE;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 0032898682     PISSN: 09252738     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1008393201236     Document Type: Article

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