High-level expression of uniformly 15N-labeled hen lysozyme in Pichia pastoris and identification of the site in hen lysozyme where phosphate ion binds using NMR measurements

FEBS Letters

Volumn 448, Issue 1, 1999, Pages 33-37

  Mine, Shouhei ^a   Ueda, Tadashi ^a   Hashimoto, Yoshio ^a   Tanaka, Yoshitsugu ^a   Imoto, Taiji ^a  

^a KYUSHU UNIVERSITY   (Japan)

Author keywords

Deamidation; Lysozyme; Phosphate ion; Pichia pastoris

Indexed keywords

ION; LYSOZYME; NITROGEN 15; PHOSPHATE;

ARTICLE; BINDING SITE; CONTROLLED STUDY; DEAMINATION; HEN; NONHUMAN; NUCLEAR MAGNETIC RESONANCE; PICHIA PASTORIS; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN EXPRESSION;

ANIMALS; BINDING SITES; CHICKENS; GENE EXPRESSION; IONS; MURAMIDASE; NITROGEN ISOTOPES; NUCLEAR MAGNETIC RESONANCE, BIOMOLECULAR; PHOSPHATES; PICHIA;

PICHIA PASTORIS;

EID: 0032991055     PISSN: 00145793     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0014-5793(99)00332-4     Document Type: Article

References (30)

1. Clarke S. Annu. Rev. Biochem. 54:1985;479-506.
2. Wright H.T. Protein Eng. 4:1991;283-294.
3. Wright H.T. CRC Crit. Rev. Biochem. 26:1991;1-52.
4. Meinwald Y.C., Stimson E.R., Scheraga H.A. Int. J. Peptide Protein. Res. 28:1986;79-84.
5. Geiger T., Clarke S. J. Biol. Chem. 262:1987;785-794.
6. Capasso S., Mazarella L., Sica F., Zagari A. Peptide Res. 2:1989;195-200.
7. Stephenson R.C., Clarke S. J. Biol. Chem. 264:1989;6164-6170.
8. Robinson A.B., Rudd C. Curr. Top. Cell Regul. 8:1974;248-295.
9. McKerrow J.H., Robinson A.B. Anal. Biochem. 42:1971;565-568.
10. Scotchler J.W., Robinson A.B. Anal. Biochem. 59:1974;319-322.
11. Tyler-Cross R., Schirch V. J. Biol. Chem. 266:1991;22549-22556.
12. Tomizawa H., Yamada H., Tanigawa K., Imoto T. J. Biochem. 117:1995;369-373.
13. Shuker S.B., Hajduk P.J., Meadows R.P., Fesik S.W. Science. 274:1996;1531-1534.
14. Hajduk P.J., Meadows R.P., Fesik S.W. Science. 278:1997;497-499.
15. Mine S., Ueda T., Hashimoto Y., Imoto T. Protein Eng. 10:1997;1333-1338.
16. Cregg J.M., Higgins D.R. Can. J. Bot. 73:1995;S891-S897.
17. Ramonas M. Curr. Opin. Biotechnol. 6:1995;527-533.
18. Cregg J.M., Vedvick T.S., Raschke W.C. Bio/Technology. 11:1993;905-910.
19. Barr K.A., Hopkins S.A., Sreekrishna K. Pharm. Eng. 12:1992;48-51.
20. Hashimoto Y., Miki T., Mukae M., Ueda T., Imoto T. Gene. 207:1998;167-170.
21. Clare J.J., Rayment F.B., Ballantine S.P., Sreekrishna K., Ramonas M. Bio/Technology. 9:1991;455-460.
22. Ito H., Fukuda Y., Murata K., Kimura A. J. Bacteriol. 153:1983;163-168.
23. Verhamme I.M., Van D.G., Lauwers A.R. Eur. J. Biochem. 172:1988;615-620.
24. Grzesiek S., Bax A. J. Am. Chem. Soc. 115:1993;12593-12594.
25. Piatto M., Saudek V., Sklenar V. J. Biomol. NMR. 2:1992;661-665.
26. Buck M., Boyd J., Redfield C., Mackenzie D.A., Jeenes D.J., Archer D.B., Dobson C.M. Biochemistry. 34:1995;4041-4055.
27. Laroche Y., Storme V., De Meutter J., Messens J., Lauwereys M. Bio/Technology. 12:1994;1119-1124.
28. Hashimoto Y., Koyabu N., Imoto T. Protein Eng. 11:1998;75-77.
29. Hashimoto Y., Koyabu N., Imoto T. Protein Peptide Lett. 5:1998;15-18.
30. Takahashi T., Nakamura H., Wada A. Biopolymers. 32:1992;897-909.