Application of amino acid type-specific 1H- and 14N-labeling in a 2H-, 15N-labeled background to a 47 kDa homodimer: Potential for nmr structure determination of large proteins

Journal of Biomolecular NMR

Volumn 14, Issue 1, 1999, Pages 79-83

  Kelly, Mark J S ^a   Krieger, Cornelia ^b   Ball, Linda J ^a   Yu, Yihua ^c,d   Richter, Gerald ^b   Schmieder, Peter ^a   Bacher, Adelbert ^b   Oschkinat, Hartmut ^a,c  

^a LEIBNIZ INSTITUT FÜR MOLEKULARE PHARMAKOLOGIE   (Germany)

^b TECHNICAL UNIVERSITY OF MUNICH   (Germany)

^c EUROPEAN MOLECULAR BIOLOGY LABORATORY   (Germany)

^d SHANGHAI INSTITUTE OF ORGANIC CHEMISTRY   (China)

Author keywords

3D heteronuclear NMR; Amino acid specific labeling; Deuteration; Heteronuclear half filter

Indexed keywords

ISOLEUCINE; PHENYLALANINE; PROTEIN; THREONINE; TYROSINE; VALINE;

ARTICLE; ISOTOPE LABELING; MOLECULAR SIZE; NUCLEAR MAGNETIC RESONANCE; NUCLEAR OVERHAUSER EFFECT; PRIORITY JOURNAL; PROTEIN DETERMINATION; PROTEIN STRUCTURE; PROTEIN TARGETING; TECHNIQUE;

AMINO ACID SEQUENCE; AMINO ACIDS; DEUTERIUM; DIMERIZATION; HYDROGEN; MOLECULAR WEIGHT; NITROGEN; NITROGEN ISOTOPES; NUCLEAR MAGNETIC RESONANCE, BIOMOLECULAR; OLIGOPEPTIDES; PROTEIN CONFORMATION; PROTEINS;

EID: 0033136436     PISSN: 09252738     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1008351606073     Document Type: Article

References (32)

1. Arrowsmith, C.H., Pachter, R., Altman, R.B., Iyer, S.B. and Jardetzky, O. (1990) Biochemistry, 29, 6332-6341.
2. Chou, P.Y. and Fasman, G.D. (1974) Biochemistry, 13, 211-222.
3. Clore, G.M. and Gronenborn, A.M. (1994) Methods Enzymol., 239, 349-363.
4. Englander, S.W. and Wand, A.J. (1987) Biochemistry, 26, 5953-5958.
5. Gardner, K.H. and Kay, L.E. (1997) J. Am. Chem. Soc., 119, 7599-7600.
6. Gardner, K.H., Rosen, M.K. and Kay, L.E. (1997) Biochemistry, 36, 1389-1401.
7. Grzesiek, S., Doebeli, H., Gentz, R., Garotta, G., Labhardt, A.M. and Bax, A. (1992) Biochemistry, 31, 8180-8190.
8. Grzesiek, S., Anglister, J., Ren, H. and Bax, A. (1993) J. Am. Chem. Soc., 115, 4369-4370.
9. Grzesiek, S. and Bax, A. (1993) J. Biomol. NMR, 3, 185-204.
10. Kay, L.E. and Gardner, K.H. (1997) Curr. Opin. Struct. Biol., 7, 722-731.
11. LeMaster, D.M. (1990) Quart. Rev. Biophys., 23, 133-174.
12. Maniatis, T., Fritsch, E.F. and Sambrook, J. (1982) Molecular Cloning. A Laboratory Manual, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
13. Metzler, W.J., Wittekind, M., Goldfarb, V., Mueller, L. and Farmer II, B.T. (1996) J. Am. Chem. Soc., 118, 6800-6801.
14. Minor, D.L. and Kim, P.S. (1994) Nature, 367, 660-663.
15. Nietlispach, D., Clowes, R.T., Broadhurst, R.W., Ito, Y., Keeler, J., Kelly, M., Ashurst, J., Oschkinat, H., Domaille, P.J. and Laue, E.D. (1996) J. Am. Chem. Soc., 118, 407-415.
16. Otting, G. and Wüthrich, K. (1990) Quart. Rev. Biophys., 23, 39-96.
17. Pittard, A.J. (1987) In Escherichia coli and Salmonella Typhimurium (Ed., Neidhardt, F.C.), Vol. 1, American Society for Microbiology, Washington, DC, pp. 368-394.
18. Reitzer, L.J. and Magasanik, B. (1987) In Escherichia coli and Salmonella Typhimurium (Ed., Neidhardt, F.C.), Vol. 1, American Society for Microbiology, Washington, DC, pp. 302-320.
19. Richter, G., Volk, R., Krieger, C., Lahm, H.-W., Röthlisberger, U. and Bacher, A. (1992) J. Bacteriol., 174, 4050-4056.
20. Richter, G., Kelly, M.J.S., Krieger, C., Yu, Y, Bermel, W., Karlsson, G., Bacher, A. and Oschkinat, H. (1999) Eur. J. Biochem., 261, 57-65.
21. Rosen, M.K., Gardner, K.H., Willis, R.C., Parris, W.E., Pawson, T. and Kay, L.E. (1996) J. Mol. Biol., 263, 627-636.
22. Shan, X., Gardner, K.H., Muhandiram, D.R., Rao, N.S. and Kay, L.E. (1996) J. Am. Chem. Soc., 118, 6570-6579.
23. Smith, B.O., Ito, Y., Raine, A., Teichmann, S., Ben-Tovim, L., Nietlispach, D., Broadhurst, R.W., Terada, T., Kelly, M., Oschkinat, H., Shibata, T., Yokoyama, S. and Laue, E.D. (1996) J. Biomol. NMR, 8, 360-368.
24. Smith, C.K., Withka, J.M. and Regan, L. (1994) Biochemistry, 33, 5510-5517.
25. Torchia, D.A., Sparks, S.W. and Bax, A. (1988a) Biochemistry, 27, 5135-5141.
26. Torchia, D.A., Sparks, S.W. and Bax, A. (1988b) J. Am. Chem. Soc., 110, 2320-2321.
27. Umbarger, H.E. (1987) In Escherichia coli and Salmonella Typhimurium (Ed., Neidhardt, F.C.), Vol. 1, American Society for Microbiology, Washington, DC, pp. 352-367.
28. Vuister, G.W., Kim, S.-J. and Bax, A. (1994) J. Am. Chem. Soc., 116, 9206-9210.
29. Wagner, G. (1993) J. Biomol. NMR, 3, 375-385.
30. Yamazaki, T., Lee, W., Arrowsmith, C.H., Muhandiram, D.R. and Kay, L.E. (1994a) J. Am. Chem. Soc., 116, 11655-11666.
31. Yamazaki, T., Lee, W., Revington, M., Mattiello, D.L., Dahlquist, F.W., Arrowsmith, C.H., Muhandiram, D.R. and Kay, L.E. (1994b) J. Am. Chem. Soc., 116, 6464-6465.
32. Zhang, H., Zhao, D., Revington, M., Lee, W., Jia, X., Arrowsmith, C.H. and Jardetzky, O. (1994) J. Mol. Biol., 238, 592-614.