Chemical protein synthesis

Current Opinion in Chemical Biology

Volumn 3, Issue 6, 1999, Pages 665-671

  Kochendoerfer, Gerd G ^a   Kent, Stephen Bh ^a  

^a Gryphon Therapeutics   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

MEMBRANE PROTEIN; METALLOPROTEIN; RANTES; SERINE PROTEINASE INHIBITOR; VIRUS PROTEIN;

HUMAN; HYDROGEN BOND; NONHUMAN; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; PROTEIN ASSEMBLY; PROTEIN CONFORMATION; PROTEIN EXPRESSION; PROTEIN SYNTHESIS; PROTEIN TERTIARY STRUCTURE; REVIEW; X RAY CRYSTALLOGRAPHY;

EID: 0032724470     PISSN: 13675931     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1367-5931(99)00024-1     Document Type: Review

References (34)

1. Dawson P.E., Muir T.W., Clark-Lewis I., Kent S.B.H. Synthesis of proteins by native chemical ligation. Science. 266:1994;776-779.
2. Hackeng T.M., Griffin J.H., Dawson P.E. Protein synthesis by native chemical ligation: expanded scope by using straightforward methodology. Proc Natl Acad Sci USA. 96:1999;10068-10073.
3. Camarero J.A., Muir T.W. Native chemical ligation of polypeptides. Curr Prot Prot Sci. 18.4:1999;1-21.
4. Dawson P.E., Churchill M., Ghadiri M.R., Kent S.B.H. Modulation of reactivity in native chemical ligation through the use of thiol additives. J Am Chem Soc. 119:1997;4325-4329.
5. Muir T.W., Dawson P.E., Kent S.B.H. Protein synthesis by chemical ligation of unprotected peptides in aqueous solution. Methods Enzymol. 289:1997;266-298.
6. Wilken J., Kent S.B.H. Chemical protein synthesis. Curr Opin Biotechnol. 9:1998;412-426.
7. Baca M., Muir T.W., Schnölzer M., Kent S.B.H. Chemical ligation of cysteine-containing peptides: synthesis of a 22 kDa tethered dimer of HIV-1 protease. J Am Chem Soc. 117:1995;1881-1887.
8. Canne L.E., Ferré-D'Amaré A.R., Burley S.K., Kent S.B.H.Total chemical synthesis of a unique transcription factor-related protein: cMyc-Max J Am Chem Soc. 117:1995;2998-3007.
9. Canne L.E., Botti P., Simon R.J., Chen Y., Dennis E.A., Kent S.B.H. Chemical protein synthesis by solid phase ligation of unprotected peptide segments. J Am Chem Soc. 121:1999;8720-8727. Taking advantage of modern solid-phase chemistry technology, the authors significantly increased the accessible range of chemically synthetic proteins by performing multiple native chemical ligations onto a resin-bound peptide.
10. Evans T.C., Benner J., Xu M.Q. Semisynthesis of cytotoxic proteins using a modified protein splicing element. Protein Sci. 11:1998;2256-2264.
11. Muir T.W., Sondhi D., Cole P.A. Expressed protein ligation: a general method for protein engineering. Proc Natl Acad Sci USA. 95:1998;6705-6710. This paper describes the application of expressed protein ligation for the incorporation of a labeled carboxy-terminal peptide into an ~600 residue protein. This demonstrates the application of a new technology to a biologically relevant problem.
12. Severinov K., Muir T.W. Expressed protein ligation, a novel method for studying protein-protein interactions in transcription. J Biol Chem. 273:1998;16205-16209.
13. Evans T., Benner J., Xu M. The in vitro ligation of bacterially expressed proteins using an intein from Methanobacterium thermoautotrophicum. J Biol Chem. 274:1999;3923-3926.
14. 15N-labeled. A neat approach to the study of protein domains in the context of larger multidomain complexes by NMR.
15. Cotton G.J., Ayers B., Xu R., Muir T.W. Insertion of a synthetic peptide into a recombinant protein framework: a protein biosensor. J Am Chem Soc. 121:1999;1100-1101.
16. Erlanson D.A., Chytil M., Verdine G.L. The leucine zipper domain controls the orientation of AP-1 in the NFAT.AP-1.DNA complex. Chem Biol. 3:1996;981-991.
17. Beligere G., Dawson P.E. Conformationally assisted protein ligation using C-terminal thioester peptides. J Am Chem Soc. 121:1999;6332-6333. Performing chemical ligation under folding conditions, the authors observed a significant increase in the rate of ligation. This technique also shows potential in overcoming some of the sequence dependence of native chemical ligation.
18. Wallace C.J. Peptide ligation and semisynthesis. Curr Opin Biotechnol. 6:1995;403-410.
19. Humphries J., Offord R.E., Smith R.A. Chemical methods of protein synthesis and modification. Curr Opin Biotechnol. 2:1991;539-543.
20. Bolin K.A., Anderson D.J., Trulson J.A., Thompson D.A., Wilken J., Kent S.B.H., Gantz I., Millhauser G.L. NMR structure of a minimized human agouti related protein prepared by total chemical synthesis. FEBS Lett. 451:1999;125-131.
21. Ueda H., Siani M.A., Gong W., Thompson D.A., Brown G.G., Wang J.M. Chemically synthesized SDF-1alpha analogue, N33A, is a potent chemotactic agent for CXCR4/Fusin/LESTR-expressing human leukocytes. J Biol Chem. 272:1997;24966-24970.
22. Dealwis C., Fernandez E.J., Thompson D.A., Simon R.J., Siani M.A., Lolis E. Crystal structure of chemically synthesized [N33A] stromal cell-derived factor 1alpha, a potent ligand for the HIV-1 fusin coreceptor. Proc Natl Acad Sci USA. 95:1998;6941-6946.
23. Shao W., Fernandez E., Wilken J., Thompson D.A., Siani M.A., West J., Lolis E., Schweitzer B.I. Accessibility of selenomethionine proteins by total chemical synthesis: structural studies of human herpesvirus-8 MIP-II. FEBS Lett. 441:1998;77-82.
24. Simmons G., Clapham P.R., Picard L., Offord R.E., Rosenkilde M.M., Schwartz T.W., Buser R., Wells T.N.C., Proudfoot A.E. Potent inhibition of HIV-1 infectivity in macrophages and lymphocytes by a novel CCR5 antagonist. Science. 276:1997;276-279.
25. Wilken J., Hoover D., Thompson D.A., Barlow P.N., McSparron H., Picard L., Wlodawer A., Lubkowski J., Kent S.B.H. Total chemical synthesis and high-resolution crystal structure of the potent anti-HIV protein AOP-RANTES. Chem Biol. 6:1999;43-51.
26. Kochendoerfer G.G., Salom D., Lear J.D., Wilk-Orescan R., Kent S.B.H., DeGrado W.F. Total chemical synthesis of the integral membrane protein influenza A virus M2: role of its C-terminal domain in tetramer assembly. Biochemistry. 38:1999;11905-11913. The total chemical synthesis of an integral membrane protein, the influenza A M2 proton channel, is reported. This is currently the most rapid way to get access to large quantities of small membrane proteins. Structure/function studies relating to the oligomerization state were carried out.
27. Lu W., Randal M., Kossiakoff A., Kent S.B.H. Probing intermolecular backbone H-bonding in serine proteinase-protein inhibitor complexes. Chem Biol. 6:1999;419-427. The use of protein 'backbone engineering' by chemical protein synthesis to systematically dissect the role of hydrogen bonds in protein-protein interactions is described. This is ture 'molecular' biology: protein structure analysis bond by bond.
28. Rau H.K., Haehnel W. Design, synthesis, and properties of a novel cytochrome b model. J Am Chem Soc. 120:1998;468-476.
29. Willner I., Heleg-Shabtai V., Katz E., Rau H., Haehnel W. Integration of a reconstituted de novo synthesized hemoprotein and native metalloproteins with electrode supports for bioelectronic and bioelectrocatalytic applications. J Am Chem Soc. 121:1999;6455-6468. A clever application of TASP (template-assembled synthetic protein) to the design of a directional electron transfer device that transfers electrons from an electrode to a redox protein. A great example of how chemical protein synthesis provides access to novel biomaterials.
30. Rau H.K., DeJonge N., Haehnel W. Modular synthesis of de novo-designed metalloproteins for light-induced electron transfer. Proc Natl Acad Sci USA. 95:1998;11526-11531.
31. Low D.W., Hill M.G. Rational fine-tuning of the redox potentials in chemically synthesized rubredoxins. J Am Chem Soc. 120:1998;11536-11537. The systematic variation of a single conserved tyrosine residue close to the redox center of rubredoxin by non-native amino acid replacement reveals the importance of this residue in the molecular mechanism of redox tuning.
32. Sydor J.R., Herrmann C., Kent S.B.H., Goody R.S., Engelhard M. Design, total chemical synthesis, and binding properties of a [Leu-91-N1-methyl-7-azaTrp]Ras-binding domain of c-Raf-1. Proc Natl Acad Sci USA. 96:1999;7865-7870.
33. Berman A.L., Kolker E., Trifonov E.N. Underlying order in protein sequence organization. Proc Natl Acad Sci USA. 91:1994;4044-4047.
34. Xu D., Nussinov R. Favorable domain size in proteins. Fold Des. 27:1997;11-17.