Genetics and molecular physiology of the yeast Kluyveromyces lactis

Fungal Genetics and Biology

Volumn 30, Issue 3, 2000, Pages 173-190

  Schaffrath, Raffael ^a   Breunig, Karin D ^a  

^a MARTIN LUTHER UNIVERSITY HALLE WITTENBERG   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

AEROBIC METABOLISM; BIOMASS; CELL RESPIRATION; DNA DAMAGE; FERMENTATION; FUNGAL GENETICS; GENETIC ENGINEERING; GLUCOSE METABOLISM; KLUYVEROMYCES; MOLECULAR GENETICS; PHYSIOLOGY; PLASMID; PRIORITY JOURNAL; REVIEW; SACCHAROMYCES CEREVISIAE; SPECIES DIFFERENCE; YEAST;

KLUYVEROMYCES LACTIS; SACCHAROMYCES CEREVISIAE;

EID: 0033764315     PISSN: 10871845     EISSN: None     Source Type: Journal    
DOI: 10.1006/fgbi.2000.1221     Document Type: Review

References (183)

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4. Yeast regulatory gene GAL3: Carbon regulation; UASGal elements in common with GAL1, GAL2, GAL7, GAL10, GAL80, and MEL1; encoded protein strikingly similar to yeast and Escherichia coli galactokinases
5. Overproduction of the GAL1 or GAL3 protein causes galactose-independent activation of the GAL4 protein: Evidence for a new model of induction for the yeast GAL/MEL regulon
6. Site-specific recombination of the circular 2 μm-like plasmid pKD1 requires integrity of the recombinase A and of the partitioning genes B and C
7. Plasmid functions involved in stable propagation of pKD1 circular plasmid in Kluyveromyces lactis
8. Glucose uptake in Kluyveromyces lactis: Role of the HGT1 gene in glucose transport
9. Novel Gal3 proteins showing altered Gal80p binding cause constitutive transcription of Gal4p-activated genes in Saccharomyces cerevisiae
10. On the safety of Kluyveromyces lactis: A review
11. Glucose repression of LAC gene expression in yeast is mediated by the transcriptional activator LAC9
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13. Regulation of primary carbon metabolism in Kluyveromyces lactis
14. Expression of the Arxula Adeninivorans glucoamylase gene in Kluyveromyces lactis
15. T-DNA from Agrobacterium tumefaciens as an efficient tool for gene targeting in Kluyveromyces lactis
16. Analysis of the response of Saccharomyces cerevisae cells to Kluyveromyces lactis toxin
17. Intracellular expression of Kluyveromyces lactis toxin γ subunit mimics treatment with exogenous toxin and distinguishes two classes of toxin-resistant mutant
18. Kluyveromyces lactis toxin has an essential chitinase activity
19. Two Saccharomyces cerevisiae genes which control sensitivity to G1 arrest induced by Kluyveromyces lactis toxin
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21. Constitutive expression in gal7 mutants of Kluyveromyces lactis is due to internal production of galactose as an inducer of the Gal/Lac regulon
22. Use of Aspergillus nidulans amdS gene as dominant selectable marker in non-conventional Kluyveromyces yeasts
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25. Specific mutations in alpha- and gamma-subunits of F1-ATPase affect mitochondrial genome integrity in the petite-negative yeast Kluyveromyces lactis
26. The mitochondrial genome integrity gene, MGII, of Kluyveromyces lactis encodes the beta-subunit of F1-ATPase
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30. The host range of the pKD1-derived plasmids in yeast
31. Sequence organization of the circular plasmid pKD1 from the yeast Kluyveromyces drosophilarum
32. Creation of a functional promoter by rearrangement in a Kluyveromyces lactis linear plasmid
33. Molecular analysis of Chs3p participation in chitin synthase III activity
34. A potential positive feedback loop controlling CLN1 and CLN2 gene expression at the start of the yeast cell cycle
35. A high frequency transformation system for the yeast Kluyveromyces lactis
36. The crabtree effect: A regulatory system in yeast
37. Nutrients, via the Tor proteins, stimulate the association of Tap42 with type 2A phosphatases
38. Glucose represses the lactose-galactose regulon in Kluyveromyces lactis through a SNF1 and M1G1-dependent pathway that modulates galactokinase (GAL1) gene expression
39. Expression of a catalytic domain of a Neocallimastix frontalis endoxylanase gene (xyn3) in Kluyveromyces lactis and Penicillium roqueforti
40. Suppressors reveal two classes of glucose repression genes in the yeast Saccharomyces cerevisiae
41. Analysis of a 1.6-micron circular plasmid from the yeast Kluyveromyces drosophilarum: Structure and molecular dimorphism
42. The elp2 subunit of elongator and elongating RNA polymerase II holoenzyme is a WD40 repeat protein
43. Heterologous protein secretion directed by a repressible acid phosphatase system of Kluyveromyces lactis: Characterization of upstream region-activating sequences in the KIPHO5 gene
44. Engineering yeast for high level expression
45. Stable multicopy vectors for high-level secretion of recombinant human serum albumin by Kluyveromyces yeasts
46. Linear DNA plasmids of yeasts
47. Identification of Kluyveromyces lactis telomerase: Discontinuous synthesis along the 30-nucleotide-long templating domain
48. Deoxyribonucleic acid base sequence relatedness among members of the genus Kluyveromyces
49. Application of yeasts in gene expression studies: A comparison of Saccharomyces cerevisiae, Hansenula polymorpha and Kluyveromyces lactis - A review
50. Transcription of the Kluyveromyces lactis invertase gene INV1 is glucose-repressed independently from MIG1
51. Difference in regulation of yeast gluconeogenesis revealed by Cat8p-independent activation of PCK1 and FBP1 genes in Kluyveromyces lactis
52. Coregulation of the Kluyveromyces lactis lactose permease and β-galactosidase genes is achieved by interaction of multiple LAC9 binding sites in a 2.6 kbp divergent promoter
53. RAG1 and RAG2: Nuclear genes involved in the dependence/independence on mitochondrial respiratory function for growth on sugars
54. RAG1 gene of the yeast Kluyveromyces lactis codes for a sugar transporter
55. FOG1 and FOG2 genes, required for the transcriptional activation of glucose-repressible genes of Kluyveromyces lactis, are homologous to GAL83 and SNF1 of Saccharomyces cerevisiae
56. Linear DNA killer plasmids from the yeast Kluyveromyces lactis
57. Plasmid DNA and the killer phenomenon in Kluyveromyces
58. Intergeneric transfer of deoxyribonucleic acid killer plasmids, pGKL1 and pGKL2, from Kluyveromyces lactis into Saccharomyces cerevisiae by cell fusion
59. Isolation and characterization of linear deoxyribonucleic acid plasmids from Kluyveromyces lactis and the plasmid-associated killer character
60. Transformation system for prototrophic industrial yeasts using the AUR1 gene as a dominant selection marker
61. Identification of the structural gene for β-glucosidase in Saccharomyces lactis
62. Centromeric DNA of Kluyveromyces lactis
63. Mutational analysis of centromeric DNA elements of Kluyveromyces lactis and their role in the species specificity of the highly homologous centromeres from K lactis and Saccharomyces cerevisiae
64. GAL4 is regulated by the RNA polymerase II holoenzyme-associated cyclin-dependent protein kinase SRB10/CDK8
65. An autoselection system in recombinant Kluyveromyces lactis enhances cloned gene stability and provides freedom in medium selection
66. Development of a LAC4 promoter-based gratuitous induction system in Kluyveromyces lactis
67. Transaldolase mutants in the yeast Kluyveromyces lactis provide evidence that glucose can be metabolized through the pentose phosphate pathway
68. Cloning and characterization of SKT5, a Saccharomyces cerevisiae gene that affects protoplast regeneration and resistance to killer toxin of Kluyveromyces lactis
69. A mutant allele skt5 affecting protoplast regeneration and killer toxin resistance has double mutations in its wild-type structural gene in Saccharomyces cerevisiae
70. Heterologous expression on the linear DNA killer plasmid from Kluyveromyces lactis
71. New recombinant linear DNA elements derived from Kluyveromyces lactis killer plasmids
72. Evolution of gene order and chromosome number in Saccharomyces, Kluyveromyces and related fungi
73. Regulation of alcoholic fermentation in batch and chemostat cultures of Kluyveromyces lactis CBS 2359
74. Hairpin plasmid - A novel linear DNA with perfect hairpin structure
75. Isolation and genetic characterization of pGKL killer-insensitive mutants (iki) from Saccharomyces cerevisiae
76. Palindrome-hairpin linear plasmids possessing only part of the ORF1 gene of the yeast killer plasmid pGKL1
77. Isolation and nucleotide sequence of the gene encoding phosphoenolpyruvate carboxykinase from Kluyveromyces lactis
78. Prevention of aerobic spoilage of maize silage by a genetically modified killer yeast, Kluyveromyces lactis, defective in the ability to grow on lactic acid
79. A general method for polyethyleneglycol-induced transformation of bacteria and yeast
80. Control of telomere growth by interactions of RAP1 with the most distal telomeric repeats
81. Rap1 protein regulates telomere turnover in yeast
82. Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit (26S) ribosomal DAN partial sequences
83. The signal for glucose repression of the lactose-galactose regulon is amplified through subtle modulation of transcription of the Kluyveromyces lactis K1GAL4 activator gene
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85. Pheromone signalling and polarized morphogenesis in yeast
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87. The SAP, a new family of proteins, associate and function positively with the SIT4 phosphatase
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89. A conserved sequence motif within the exceptionally diverse telomeric sequences of budding yeasts
90. Cap-prevented recombination between terminal telomeric repeat arrays (telomere CPR) maintains telomeres in Kluyveromyces lactis lacking telomerase
91. Terminal region recognition factor 1, a DNA binding protein recognising the ITRs of the pGKL linear plasmids
92. High efficiency transformation of intact yeast cells by electric field pulses
93. Microbial linear plasmids
94. A novel approach to express a heterologous gene Kluyveromyces lactis linear killer plasmids: Expression of the bacterial aph gene from a cytoplasmic promoter fragment not fused in frame
95. CPF1, a yeast protein with functions in centromeres and promoters
96. Galactokinase encoded by GAL1 is a bifunctional protein required for induction of the GAL genes in Kluyveromyces lactis and is able to suppress the gal3 phenotype in Saccharomyces cerevisiae
97. Carbon catabolite regulation of transcription of nuclear genes coding for mitochondrial proteins in the yeast Kluyveromyces lactis
98. Centromere promter factors (CPF1) of the yeasts of Saccharomyces cerevisiae and Kluyveromyces lactis are functionally exchangeable despite low overall homology
99. Comparison of secretion of a hepatitis C virus glycoprotein in Saccharomyces cerevisiae and Kluyveromyces lactis
100. Regulated phosphorylation and dephosphorylation of GAL4, a transcriptional activator
101. Control of yeast GAL genes by MIG1 repressor: A transcriptional cascade in the glucose response
102. Curing of the killer dexyribonucleic acid plasmids of Kluyveromyces lactis
103. A nonanucleotide sequence involved in promotion of ribosomal RNA synthesis and RNA priming of DNA replication in yeast
104. Elongator, a multisubunit component of a novel RNA polymerase II holoenzyme for transcriptional elongation
105. Random exploration of the Kluyveromyces lactis genome and comparison with that of Saccharomyces cerevisiae
106. The yeast galactose genetic switch is mediated by the formation of a Gal4p-Gal80p-Gal3p complex
107. Heterologous gene expression on the linear DNA plasmids of Kluyveromyces lactis
108. Expression and secretion of recombinant ovine beta-lactoglobulin in Saccharomyces cerevisiae and Kluyveromyces lactis
109. A transcriptional barrier to expression of cloned toxin genes of the linear plasmid k1 of Kluyveromyces lactis: Evidence that native k1 has novel promoters
110. Specific telomerase RNA residues distant from the template are essential for telomerase function
111. Positive regulation of the β-galactosidase gene from Kluyveromyces lactis is mediated by an upstream activation site that shows homology to the GAL upstream activation site of Saccharomyces cerevisiae
112. Phosphorylation of Gal4p at a single C-terminal residue is necessary for galactose-inducible transcription
113. GAL4 is phosphorylated as a consequence of transcriptional activation
114. Protein priming of DNA replication
115. Use of the KlADH4 promoter for ethanol-dependent production of recombinant human serum albumin in Kluyveromyces lactis
116. Analysis of the Kluyveromyces lactis positive regulatory gene LAC9 reveals functional homology to, but sequence divergence from, the Saccharomyces cerevisiae GAL4 gene
117. Targeting of chitin synthase 3 to polarized growth sites in yeast requires Chs5p and Myo2p
118. Kluyveromyces lactis killer plasmid pGKL2: Molecular analysis of an essential gene, ORF5
119. A cytoplasmic gene shuffle system in Kluyveromyces lactis: Use of epitope-tagging to detect a killer plasmid encoded gene product
120. Yeast killer plasmid pGKL2: Molecular analysis of UCS5, a cytoplasmic promoter element essential for ORF5 gene function
121. ORF7 of yeast plasmid pGKL2: Analysis of gene expression in vivo
122. Genetic manipulation of Kluyveromyces lactis linear DNA plasmids: Gene tageting and plasmid shuffles
123. Use of gene shuffles to study the cytoplasmic transcription system operating on Kluyveromyces lactis linear DNA plasmids
124. The DNA and RNA polymerase genes of yeast plasmid pGKL2 are essential loci for plasmid integrity and maintenance
125. Cytoplasmic gene expression in yeast: A plasmid-encoded transcription system in Kluyveromyces lactis
126. Toxin-mediated cell cycle arrest in yeast: The killer phenomenon of Kluyveromyces lactis
127. Kluyveromyces lactis killer system: ORF1 of pGKL2 has no function in immunity expression and is dispensable for killer plasmid replication and maintenance
128. Kluyveromyces lactis killer system: Analysis of cytoplasmic promoters of the linear plasmids
129. Extranuclear expression of the bacterial xylose-isomerase (xylA) and UDP-glucose-dehydrogenase (hasB) genes in yeast using Kluyveromyces lactis linear killer plasmids as vectors
130. Extent of genomic rearrangement after genome duplication in yeast
131. Mutations affecting synthesis of β-Galactosidase activity in the yeast Kluyveromyces lactis
132. Sequence of the Kluyveromyces lactis URA3 gene
133. Uncapping and deregulation of telomeres lead to detrimental cellular consequences in yeast
134. Analysis of chromosome patterns of the genus Kluyveromyces
135. Construction of strains of Saccharomyces cerevisiae that grow on lactose
136. Transformation of Kluyveromyces lactis with the kanamycin (G418) resistance gene of Tn903
137. Yeast protein serine/threonine phosphatases: Multiple roles and diverse regulation
138. The killer toxin of Kluyveromyces lactis: Characterization of the toxin subunits and identification of the genes which encode them
139. Cloning and analysis of the K lactis TRP1 gene: A chromosomal locus flanked by genes encoding inorganic pyrophosphatase and histone H3
140. The plasmid-encoded killer system of Kluyveromyces lactis: A review
141. Characterization of a novel killer toxin encoded by a double-stranded linear DNA killer plasmid of Kluyveromyces lactis
142. The yeast Kluyveromyces lactis as an efficient host for heterologous gene expression
143. Absence of cell wall chitin in Saccharomyces cerevisiae leads to resistance to Kluyveromyces lactis killer toxin
144. The Kluyveromyces lactis KEX1 gene encodes a subtilisin-type serin proteinase
145. Expression of a foreign Km(R) gene in a linear killer DNA plasmid in yeast
146. Physical separation and functional interaction of Kluyveromyces lactis and Saccharomyces cerevisiae ARS elements derived from killer plasmid DNA
147. A novel yeast secretion vector utilizing secretion signal of killer toxin encoded on the yeast linear DNA plasmid pGKL1
148. A novel yeast secretion signal isolated from 28K killer precursor protein encoded on the linear DNA plasmid pGKL1
149. Expression of pGKL killer 28K subunit in Saccharomyces cerevisiae: Identification of 28K subunit as a killer protein
150. Secretion of killer toxin encoded on the linear DNA plasmid pGKL1 from Saccharomyces cerevisae
151. Characterization of mouse α-amylase secreted from Saccharomyces cerevisae by the pGKL 128 kDa killer secretion signal
152. Secretion of mouse α-amylase from Kluyveromyces lactis
153. Chs7p, a new protein involved in the control of protein export from the endoplasmic reticulum that is specifically engaged in the regulation of chitin synthesis in Saccharomyces cerevisiae
154. Characterization of CHS4 (CAL2), a gene of Saccharomyces cerevisiae involved in chitin biosynthesis and allelic to SKT5 and CSD4
155. A differential medium for the isolation of Kluyveromyces marxianus and Kluyveromyces lactis from dairy products
156. Kluyveromyces as a host for heterologous gene expression: Expression and secretion of prochymosin
157. Taxonomic revision of the yeast genus Kluyveromyces by nuclear deoxyribonucleic acid reassociation
158. Physiology of yeasts in relation to biomass yields
159. Galactose induction in yeast involves association of Gal80p with Gal1p or Gal3p
160. Expression and secretion of a xylanase from the extreme thermophile. Thermotoga strain FjSS3B.1
161. The organization and transcription of the galactose gene cluster of Kluyveromyces lactis
162. Influence of mutations in hexose-transporter genes on glucose repression in Kluyveromyces lactis
163. Kluyveromyces lactis
164. A nuclear gene required for the expression of the linear DNA-associated killer system in the yeast Kluyveromyces lactis
165. Kluyveromyces lactis toxin does not inhibit yeast adenylyl cyclase
166. Extranuclear gene expression in yeast: Evidence for a plasmid-encoded RNA polymerase of unique structure
167. The lysine-rich C-terminal repeats of the centromere-binding factor (Cbf5) of Kluyveromyces lactis are not essential for function
168. A novel histone acetyltransferase is an integral subunit of elongating RNA polymerase II holoenzyme
169. Molecular evidence for an ancient duplication of the entire yeast genome
170. Characterization of a positive regulatory gene, LAC9, that controls induction of the lactose-galactose regulon of Kluyveromyces lactis: Structural and functional relationships to GAL4 of Saccharomyces cerevisiae
171. Characterization of lK11 and lK13 genes conferring pGKL killer sensitivity on Saccharomyces cerevisiae
172. Galactose-dependent reversible interaction of Gal3p with Gal80p in the induction pathway of Gal4p-activated genes of Saccharomyces cerevisiae
173. Expression of the transcriptional activator LAC9 (KlGAL4) in Kluyveromyces lactis is controlled by autoregulation
174. Glucose repression of lactose/galactose metabolism in Kluyveromyces lactis is determined by the concentration of the transcriptional activator LAC9 (KlGAL4)
175. Inactivation of the Kluyveromyces lactis KlPDAI gene leads to loss of pyruvate dehydrogenase activity, impairs growth on glucose and triggers aerobic alcoholic fermentation
176. Regulated phosphorylation of the Gal4p inhibitor Gal80p of Kluyveromyces lactis revealed by mutational analysis
177. Gal80 proteins of Kluyveromyces lactis and Saccharomyces cerevisiae are highly conserved but contribute differently to glucose repression of the galactose regulon
178. Activation of Gal4p by galactose-dependent interaction of galactokinase and Gal80p
179. LEU2 gene homolog in Kluyveromyces lactis
180. Chs1p and Chs3p, two proteins involved in chitin synthesis, populate a compartment of the Saccharomyces cerevisiae endocytic pathway
181. Chs6p-dependent anterograde transport of Chs3p from chitosomes to the plasma membrane in Saccharomyces cerevisiae