An autoselection system in recombinant Kluyveromyces lactis enhances cloned gene stability and provides freedom in medium selection

Applied Microbiology and Biotechnology

Volumn 49, Issue 2, 1998, Pages 147-152

  Hsieh, H P ^a   Da Silva, N A ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; BACTERIAL GROWTH; BACTERIUM CULTURE; CULTURE MEDIUM; DNA TRANSFER; GENETIC STABILITY; KLUYVEROMYCES; MUTANT; PLASMID; TECHNIQUE;

BETA-FRUCTOFURANOSIDASE; CLONING, MOLECULAR; CULTURE MEDIA; GENE EXPRESSION; GENES, FUNGAL; GLYCOSIDE HYDROLASES; KLUYVEROMYCES; MUTATION; PLASMIDS; RECOMBINANT PROTEINS; TRANSFORMATION, GENETIC;

BACTERIA (MICROORGANISMS); KLUYVEROMYCES LACTIS; SACCHAROMYCES CEREVISIAE;

EID: 0031908849     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s002530051151     Document Type: Article

References (24)

1. Ayub M, Astolfi-Filho S, Mavituna F, Oliver S (1992) Studies on plasmid stability, cell metabolism and superoxide dismutase production by Pgk- strains of Saccharomyces cerevisiae. Appl Microbiol Biotechnol 37: 615-620
2. Bianchi MM, Falcone C, Chen XJ, Weslowski-Louvel M, Frontali L, Fukuhara H (1987) Transformation of the yeast Kluyveromyces lactis by new vectors derived from the 1.6 μm circular plasmid pKD1. Curr Genet 12: 185-192
3. Bianchi MM, Santarelli R, Frontali L (1991) Plasmid functions involved in the stable propagation of the pKD1 circular plasmid in Kluyveromyces lactis. Curr Genet 19: 151-161
4. Bolen PL, McCutchan JE (1992) Electroporation of the yeast Kluyveromyces lactis. Biotechnol Tech 6: 283-286
5. Compagno C, Tura A, Ranzi BM, Alberghina L, Martegani E (1993) Copy number modulation in an autoselection system for stable plasmid maintenance in Saccharomyces cerevisiae. Biotechnol Prog 9: 594-599
6. Emr SD, Schekman R, Flessel MC, Thorner J (1983) An MFxl-SUC2 (x-factor-invertase) gene fusion for study of protein localization and gene expression in yeast. Proc Natl Acad Sci USA 80: 7080
7. Fleer R (1992) Engineering yeast for high level expression. Curr Opin Biotechnol 3: 486-496
8. Hsieh, H.-P., Da Silva, NA (1998) Partial-pKD1 plasmids provide enhanced structural stability for heterologous protein production in Kluyveromyces lactis. Applied Microbiol Biotechnol in press
9. Jones EW, Fink GR (1982) Regulation of amino acid and nucleotide biosynthesis in yeast. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
10. Jund R, Lacroute F (1970) Genetic and physiological aspects of resistance to 5-fluoropyrimidines in Saccharomyces cerevisiae. J Bacteriol 102: 607-615
11. Loison G, Nguyen-Juillert M, Alouani S, Marquet M (1986) Plasmid-transformed ura3 furl double-mutants of Saccharomyces cerevisiae: an autoselection system applicable to the production of foreign proteins. Biotechnology 4: 433
12. Loison G, Vidal A, Findeli A, Roitsch C, Balloul J, Lemoine Y (1989) High level expression of a protective antigen of schistosomes in Saccharomyces cerevisiae. Yeast 5: 497-507
13. Marquet M, Alouani S, Haas M, Loison G, Brown S (1986) Plasmid stability during continuous culture in a Saccharomyces cerevisiae double mutant transformed by a plasmid carrying an eukaryotic gene. Biotechnol Lett 8: 535-540
14. Marquet M, Alouani S, Haas M, Loison G, Brown S (1987) Double mutants of Saccharomyces cerevisiae harbour stable plasmids: stable expression of an eukaryotic gene and the influence of host physiology during continuous culture. J Biotechnol 6: 135-145
15. Napp SJ, Da Silva NA (1992) Enhancement of cloned gene product synthesis via autoselection in recombinant Saccharomyces cerevisiae. Biotechnol Bioeng 41: 801
16. Piper P, Curran B (1990) When a glycolytic gene on a yeast 2μ ORI-STB plasmid is made essential for growth its expression level is a major determinant of plasmid copy number. Curr Genet 17: 119-123
17. Rech SB, Stateva LB, Oliver SG (1992) Complementation of the Saccharomyces cerevisiae srb1-1 mutation: an autoselection system for stable plasmid maintenance. Curr Genet 21: 339-344
18. Romanos MA, Scorer CA, Clare JJ (1992) Foreign gene expression in yeast: a review. Yeast 8: 423-488
19. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
20. Sherman F, Fink GR, Hicks J (1986) Methods in yeast genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
21. Swinkels BW, Ooyen AJJ van, Bonekamp FJ (1993) The yeast Kluyveromyces lactis as an efficient host for heterologous gene expression. Antonie van Leeuwenhoek 64: 187-201
22. Unternahrer S, Pridmore D, Hinnen A (1991) A new system for amplifying 2μm plasmid copy number in Saccharomyces cerevisiae. Mol Microbiol 5: 1539-1548
23. Wang Z, Da Silva N (1993) Improved protein synthesis and secretion through medium enrichment in a stable recombinant yeast strain. Biotechnol Bioeng 42: 95-102
24. Wesolowski-Louvel M, Breunig KD, Fukuhara H (1996) Kluyveromyces lactis. In: Wolf K (eds) Genetics, biochemistry, and molecular biology of non-conventional yeasts. Springer, Berlin Heidelberg New York, pp 139-201