The yeast galactose genetic switch is mediated by the formation of a Gal4p-Gal80p-Gal3p complex

EMBO Journal

Volumn 17, Issue 14, 1998, Pages 4086-4091

  Platt, Adam ^a   Reece, Richard J ^a  

^a UNIVERSITY OF MANCHESTER   (United Kingdom)

Author keywords

Gal4p; Galactose; Gene regulation; Genetic switch; In vitro transcription

Indexed keywords

ADENOSINE TRIPHOSPHATE; CARBON; GALACTOSE; TRANSACTIVATOR PROTEIN;

ARTICLE; GENE ACTIVATION; GENE INDUCTION; GENE REPRESSION; NONHUMAN; PRIORITY JOURNAL; PROTEIN PROTEIN INTERACTION; SACCHAROMYCES CEREVISIAE; YEAST;

ADENOSINE TRIPHOSPHATE; DNA, FUNGAL; FUNGAL PROTEINS; GALACTOKINASE; GALACTOSE; GENE EXPRESSION REGULATION, FUNGAL; REPRESSOR PROTEINS; RNA, FUNGAL; RNA, MESSENGER; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; TRANS-ACTIVATION (GENETICS); TRANSCRIPTION FACTORS;

SACCHAROMYCES CEREVISIAE;

EID: 0032527810     PISSN: 02614189     EISSN: None     Source Type: Journal    
DOI: 10.1093/emboj/17.14.4086     Document Type: Article

References (23)

1. Bhat,P.J. and Hopper,J.E. (1991) The mechanism of inducer formation in ga13 mutants of the yeast galactose system is independent of normal galactose metabolism and mitochondrial respiratory function. Genetics, 128, 233-239.
2. Bhat,P.J. and Hopper,J.E. (1992) Overproduction of the GAL1 or GAL3 protein causes galactose-independent activation of the GAL4 protein: evidence for a new model of induction for the yeast GAL/MEL regulon. Mol. Cell. Biol., 12, 2701-2707.
3. Bhat,P.J., Oh,D. and Hopper,J.E. (1990) Analysis of the GAL3 signal transduction pathway activating GAL4 protein-dependent transcription in Saccharomyces cerevisiae. Genetics, 125, 281-291.
4. Blank,T.E., Woods,M.P., Lebo,C.M., Xin,P. and Hopper,J.E. (1997) Novel Gal3 proteins showing altered Gal80p binding cause constitutive transcription of Gal4p-activated genes in Saccharomyces cerevisiae. Mol. Cell. Biol., 17, 2566-2575.
5. Giniger,E., Varnum,S.M. and Ptashne,M. (1985) Specific DNA binding of GAL4, a positive regulatory protein of yeast. Cell, 40, 767-774.
6. Hashimoto,H., Kikuchi,Y., Nogi,Y. and Fukasawa,T. (1983) Regulation of expression of the galactose gene cluster in Saccharomyces cerevisiae. Isolation and characterization of the regulatory gene GAL4. Mol. Gen. Genet., 191, 31-38.
7. Johnston,M. (1987) A model fungal gene regulatory mechanism: The GAL genes of Saccharomyces cerevisiae. Microbiol. Rev., 51, 458-476.
8. Leuther,K.K. and Johnston,S.A. (1992) Nondissociation of GAL4 and GAL80 in vivo after galactose induction. Science, 256, 1333-1335.
9. Lillie,J.W., Green,M. and Green,M.R. (1986) An adenovirus E1a protein region required for transformation and transcriptional repression. Cell, 46, 1043-1051.
10. Lin,Y.-S., Carey,M.F., Ptashne,M. and Green,M.R. (1988) GAL4 derivatives function alone and synergistically with mammalian activators in vitro. Cell, 54, 659-664.
11. Lohr,D., Venkov,P. and Zlantanova,J. (1995) Transcriptional regulation in the yeast GAL gene family: A complex genetic network. FASEB J., 9, 777-787.
12. Lue,N.F., Chasman,D.I., Buchman,A.R. and Kornberg,R.D. (1987) Interaction of GAL4 and GAL80 gene regulatory proteins in vitro. Mol. Cell. Biol., 7, 3446-3451.
13. Nogi,Y. and Fukasawa,T. (1984) Nucleotide sequence of the yeast regulatory gene GAL80. Nucleic Acids Res., 12, 9287-9298.
14. Nogi,Y., Shimada,H., Matsuzaki,Y., Hashimoto,H. and Fukasawa,T. (1984) Regulation of expression of the galactose gene cluster in Saccharomyces cerevisiae. II. The isolation and dosage effect of the regulatory gene GAL80. Mol. Gen. Genet., 195, 29-34.
15. Ohashi,Y., Brickman,J.M., Furman,E., Middleton,B. and Carey,M. (1994) Modulating the potency of an activator in a yeast in vitro transcription system. Mol. Cell. Biol., 13, 2731-2739.
16. 6 zinc cluster proteins. Science, 261, 909-911.
17. Reece,R.J. and Platt,A. (1997) Signaling activation and repression of RNA polymerase II transcription in yeast. BioEssays, 19, 1001-1010.
18. Reece,R.J., Rickles,R.J. and Ptashne,M. (1993) Overproduction and single-step purification of GAL4 fusion proteins from Escherichia coli. Gene, 126, 105-107.
19. Rotman,B. and Spiegelman,S. (1953) The conversion of negatives to positives in 'slow' adapting populations of yeast, J. Bacterial., 66, 492-497.
20. Suzuki-Fujimoto,T., Fukuma,M., Yano,K.-I., Sakurai,H., Vonika,A., Johnston,S.A. and Fukasuwa,T. (1996) Analysis of the galactose signal transduction pathway in Saccharomyces cerevisiae: Interaction between Gal3p and Gal80p, Mol. Cell. Biol., 16, 2504-2508.
21. Wu,Y., Reece,R.J. and Ptashne,M. (1996) Quantitation of putative activator-target affinities predicts transcriptional activating potentials. EMBO J., 15, 3951-3963.
22. Yano,K.I. and Fukasawa,T. (1997) Galactose-dependent reversible interaction of Gal3p with Gal80p in the induction pathway of Ga4p-activated genes of Saccharomyces cerevisiae. Proc. Natl Acad. Sci. USA, 94, 1721-1726.
23. Zenke,F.T., Engles,R., Vollenbroich,V., Meyer,J., Hollenberg,C.P. and Breunig,K.D. (1996) Activation of Gal4p by galactose-dependent interaction of galactokinase and Gal80p. Science, 272, 1662-1665.