Use of gene shuffles to study the cytoplasmic transcription system operating on Kluyveromyces lactis linear DNA plasmids

Enzyme and Microbial Technology

Volumn 26, Issue 9-10, 2000, Pages 664-670

  Schaffrath, Raffael ^a   Sasnauskas, Kestutis ^b   Meacock, Peter A ^c  

^a HARVARD MEDICAL SCHOOL   (United States)

^b VILNIUS UNIVERSITY   (Lithuania)

^c UNIVERSITY OF LEICESTER   (United Kingdom)

Author keywords

Gene shuffle; Kluyveromyces lactis; Linear plasmids; RNA polymerase; UCS promoter

Indexed keywords

DNA; ENZYMES; GENES; GROWTH KINETICS; RNA; YEAST;

CYTOPLASMIC TRANSCRIPTION SYSTEM; KLUYVEROMYCES LACTIS; POLYMERASE;

GENETIC ENGINEERING;

PLASMID DNA; TRANSCRIPTION FACTOR;

CONFERENCE PAPER; CYTOPLASM; GENE DISRUPTION; GENE EXPRESSION; GENE FUNCTION; GENE FUSION; GENETIC TRANSCRIPTION; KLUYVEROMYCES; NONHUMAN; OPEN READING FRAME; POLYMERASE CHAIN REACTION; RECOMBINANT PLASMID;

KLUYVEROMYCES LACTIS;

EID: 0034214327     PISSN: 01410229     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0141-0229(00)00157-5     Document Type: Conference Paper

References (32)

1. Cong Y.S., Yarrow D., Li Y.-Y., Fukuhara H. Linear DNA plasmids from Pichia etchelsii, Debaryomyces hansenii and Wingea robertsiae. Microbiology. 140:1994;1327-1335.
2. Fukuhara, H. Linear DNA plasmids of yeasts. FEMS Microbiol. Lett 1995;131:1-9.
3. Meinhardt F., Schaffrath R., Larsen M. Microbial linear plasmids. Appl Microbiol Biotechnol. 47:1997;329-336.
4. Schaffrath R., Meinhardt F. Das Killersystem von Kluyveromyces lactis Molekularbiologie und Biotechnik . BIOspektrum. 4:1998;40-42.
5. Schaffrath R., Stark M.J.R., Struhl K. Toxin-mediated cell cycle arrest in yeast the killer phenomenon of Kluyveromyces lactis . BIOforum Int. 1:1997;83-85.
6. Wilson D.W., Meacock P.A. Extranuclear gene expression in yeast evidence for a plasmid-encoded RNA polymerase of unique structure . Nucleic Acids Res. 16:1988;8097-8112.
7. Stark M.J.R., Boyd A., Mileham A.J., Romanos M.A. The plasmid-encoded killer system of Kluyveromyces lactis a review . Yeast. 6:1990;1-29.
8. Romanos M., Boyd A.A. transcriptional barrier to expression of cloned toxin genes of the linear plasmid k1 of Kluyveromyces lactis evidence that native k1 has novel promoters . Nucleic Acids Res. 16:1988;7333-7350.
9. Kämper J., Esser K., Gunge N., Meinhardt F. Heterologous expression on the linear DNA killer plasmid from Kluyveromyces lactis. Curr Genet. 19:1991;109-118.
10. Gunge N., Fukuda K., Takahashi S., Meinhardt F. Migration of the yeast linear DNA plasmid from the cytoplasm to the nucleus in Saccharomyces cerevisiae. Curr Genet. 28:1995;280-288.
11. Hishinuma F., Hirai K. Genome organisation of the linear plasmid pSKL isolated from Saccharomyces kluyveri. Mol Gen Genet. 226:1991;97-106.
12. Schaffrath R., Soond S.M., Meacock P.A. Cytoplasmic gene expression in yeast a plasmid-encoded transcription system in Kluyveromyces lactis . Biochem Soc Trans. 23:1995;128.
13. Schaffrath R., Meinhardt F., Meacock P.A. ORF7 of yeast plasmid pGKL2 analysis of gene expression in vivo . Curr Genet. 31:1997;190-192.
14. Schaffrath R., Soond S.M., Meacock P.A. The DNA and RNA polymerase genes of yeast plasmid pGKL2 are essential loci for plasmid integrity and maintenance. Microbiology. 141:1995;2591-2599.
15. Sherman F. Getting started with yeast. Methods Enzymol. 194:1991;3-21.
16. Sambrook J, Fritsch EF, Maniatis T. Molecular cloning: a laboratory manual, 2nd edition. Cold Spring Harbor, NY: CSHL Press, 1989.
17. Schaffrath R., Meinhardt F., Meacock P.A. Yeast killer plasmid pGKL2 molecular analysis of UCS5, a cytoplasmic promoter element essential for ORF5 gene function . Mol Gen Genet. 250:1996;286-294.
18. Tommasino M., Ricci S., Galeotti C. Genome organisation of the plasmid pGKL2 from Kluyveromyces lactis. Nucleic Acids Res. 16:1988;5863-5878.
19. Schaffrath R., Stark M.J.R., Gunge N., Meinhardt F. Kluyveromyces lactis killer system ORF1 of pGKL2 has no function in immunity expression and is dispensable for killer plasmid replication and maintenance . Curr Genet. 21:1992;357-363.
20. Schaffrath R., Meacock P.A. A cytoplasmic gene shuffle system in Kluyveromyces lactis use of epitope-tagging to detect a killer plasmid-encoded gene product . Mol Microbiol. 19:1996;545-554.
21. Schaffrath R. The killer system of Kluyveromyces lactis: molecular genetic analysis of killer plasmid k2 gene function. Leicester, UK: University of Leicester, 1995. Thesis.
22. Schaffrath R., Meacock P.A. Kluyveromyces lactis killer plasmid pGKL2 molecular analysis of an essential gene, ORF5 . Yeast. 11:1995;615-628.
23. Feinberg A.P., Vogelstein B. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem. 132:1983;6-13.
24. Meinhardt F., Wodara C., Larsen M., Schickel J. A novel approach to express a heterologous gene Kluyveromyces lactis linear killer plasmids expression of the bacterial aph gene from a cytoplasmic promoter fragment not fused in frame . Plasmid. 32:1994;318-327.
25. Schründer J., Meinhardt F. An extranuclear expression system for analysis of cytoplasmic promoters of yeast linear killer plasmids. Plasmid. 33:1995;139-151.
26. Bolen P.L., Eastman E.M., Cihak P.L., Hayman C.T. Isolation and sequence analysis of a gene from the linear DNA plasmid pPac 1-2 of Pichia acaciae that shows similarity to a killer toxin gene of Kluyveromyces lactis. Yeast. 10:1994;403-414.
27. Fukada K., Maebuchi M., Takata H., Gunge N. The linear plasmid pDHL1 from Debaryomyces hansenii encodes a protein highly homologous to the pGKL1-plasmid DNA polymerase. Yeast. 13:1997;613-620.
28. Cong Y.-S., Wesolowski-Louvel M., Fukuhara H. Creation of a functional promoter by rearrangenment in a Kluyveromyces lactis linear plasmid. Gene. 147:1994;125-129.
29. Schickel J., Helmig C., Meinhardt F. Kluyveromyces lactis killer system analysis of cytoplasmic promoters of the linear plasmids . Nucleic Acids Res. 24:1996;1879-1886.
30. R gene in a linear killer DNA plasmid in yeast. Gene. 91:1990;43-50.
31. Schründer J., Gunge N., Meinhardt F. Extranuclear expression of the bacterial xylose-isomerase (xylA) and UDP-glucose-dehydrogenase (hasB) genes in yeast using Kluyveromyces lactis linear killer plasmids as vectors. Curr Microbiol. 33:1996;323-330.
32. Reliene R., Sasnauskas K. Heterologous gene expression on the linear DNA plasmids of Kluyveromyces lactis. Yeast SI. 13:1997;S227.