The URA5 gene encoding orotate-phosphoribosyl transferase of the yeast Kluyveromyces lactis: Cloning, sequencing and use as a selectable marker

Yeast

Volumn 15, Issue 13, 1999, Pages 1393-1398

  Bai, Xiyuan ^a,b   Larsen, Michael ^a   Meinhardt, Friedhelm ^a  

^a UNIVERSITY OF MÜNSTER   (Germany)

^b XI'AN JIAOTONG UNIVERSITY   (China)

Author keywords

Kluyveromyces; Orotate phosphoribosyltransferase; SEC65; URA5

Indexed keywords

OROTATE PHOSPHORIBOSYLTRANSFERASE; PRIMER DNA; URACIL;

ARTICLE; CULTURE MEDIUM; FUNGAL GENETICS; GENE AMPLIFICATION; GENE SEQUENCE; GENETIC CONSERVATION; KLUYVEROMYCES; MARKER GENE; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; PRIORITY JOURNAL; SACCHAROMYCES CEREVISIAE;

AMINO ACID SEQUENCE; BASE SEQUENCE; CLONING, MOLECULAR; GENES, FUNGAL; KLUYVEROMYCES; MOLECULAR SEQUENCE DATA; OROTATE PHOSPHORIBOSYLTRANSFERASE;

EID: 0033619122     PISSN: 0749503X     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0061(19990930)15:13<1393::AID-YEA452>3.0.CO;2-F     Document Type: Article

References (22)

1. Bonekamp, F. J. and Oosterom, J. (1994). On the safety of Kluyveromyces lactis - a review. Appl. Microbiol. Biotechnol. 41, 1-3.
2. Boy-Marcotte, E., Vilaine, F., Camonis, J. and Jacquet, M. (1984). A DNA sequence from Dictyostelium discoideum complements ura3 and ura5 mutations of Saccharomyces cerevisiae. Mol. Gen. Genet. 193, 406-413.
3. Caroline, D. F. (1969). Pyrimidine synthesis in Neurospora crassa: gene-enzyme relationships. J. Bacteriol. 100, 1371-1377.
4. Fleer, R., Chen, X. J., Amellal, N., Yeh, P., Fournier, A., Guinet, F., Gault, N., Faucher, D., Folliard, F., Fukuhara, H. et al. (1991a). High-level secretion of correctly processed recombinant human interleukin-1 beta in Kluyveromyces lactis. Gene 107, 285-295.
5. Fleer, R., Yeh, P., Amellal, N., Maury, I., Fournier, A., Bacchetta, F., Baduel, P., Jung, G., L'Hote, H., Becquart, J. et al. (1991b). Stable multicopy vectors for high-level secretion of recombinant human serum albumin by Kluyveromyces yeasts. Biotechnology 9, 968-975.
6. Gietz, R. D. and Schiestl, R. H. (1995). Transforming yeast with DNA. Methods Mol. Cell. Biol. 5, 255-269.
7. Jones, M. E. (1980). Pyrimidine nucleotide biosynthesis in animals: genes, enzymes, and regulation of UMP biosynthesis. Ann. Rev. Biochem. 49, 253-279.
8. Jund, R. and Lacroute, F. (1972). Regulation of orotidylic acid pyrophosphorylase in Saccharomyces cerevisiae. J. Bacteriol. 109, 196-202.
9. de-Montigny, J., Kern, L., Hubert, J. C. and Lacroute, F. (1990). Cloning and sequencing of URA10, a second gene encoding orotate phosphoribosyl transferase in Saccharomyces cerevisiae. Curr. Genet. 17, 105-111.
10. O'Donovan, G. A. and Neuhard, J. (1970). Pyrimidine metabolism in microorganisms. Bacteriol. Rev. 34, 278-343.
11. Regnacq, M., Hewitt, E., Allen, R. J. and Stirling, C. J. (1998). Deletion analysis of yeast Sec65p reveals a central domain that is sufficient for function in vivo. Mol. Microbiol. 29, 753-762.
12. Romanos, M. A., Scorer, C. A. and Clare, J. J. (1992). Foreign gene expression in yeast: a review. Yeast 8, 423-488.
13. Sambrook, J., Fritsch, E. F. and Maniatis, T. (1989). Molecular Cloning. A Laboratory Manual, 2nd edn. Cold Spring Harbor Laboratory Press, New York.
14. Sanchez, M., Prado, M., Iglesias, F. J. and Dominguez, A. (1995). Cloning and sequencing of the URA5 gene from the yeast Yarrowia lipolytica. Yeast 11, 425-433.
15. Sanchez, M., Beckerich, J. M., Gaillardin, C. and Dominguez, A. (1997). Isolation and cloning of the Yarrowia lipolytica SEC65 gene, a component of the yeast signal recognition particle displaying homology with the human SRP19 gene. Gene 203, 75-84.
16. Sherman, F., Fink, G. R. and Hicks, J. B. (1986). Laboratory Course Manual for Methods in Yeast Genetics, 2nd edn. Cold Spring Harbor Laboratory Press, New York.
17. Shuster, J. R., Moyer, D. and Irvine, B. (1987). Sequence of the Kluyveromyces lactis URA3 gene. Nucleic Acids Res. 15, 8573.
18. Sreekrishna, K., Webster, T. D. and Dickson, R. C. (1984). Transformation of Kluyveromyces lactis with the kanamycin (G418) resistance gene of Tn903. Gene 28, 73-81.
19. Stirling, C. J. and Hewitt, E. W. (1992). The S. cerevisiae SEC65 gene encodes a component of yeast signal recognition particle with homology to human SRP19. Nature 356, 534-537.
20. Tanguy-Rougeau, C., Chen, X. J., Wesolowski-Louvel, M. and Fukuhara, H. (1990). Expression of a foreign KmR gene in linear killer DNA plasmids in yeast. Gene 91, 43-50.
21. van den Berg, J. A., van der Laken, K. J., van Ooyen, A. J., Renniers, T. C., Rietveld, K., Schaap, A., Brake, A. J., Bishop, R. J., Schultz, K. and Moyer, D. et al. (1990). Kluyveromyces as a host for heterologous gene expression: expression and secretion of prochymosin. Biotechnology 8, 135-139.
22. Yanisch-Perron, C., Vieira, J. and Messing, J. (1985). Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33, 103-119.