Functional analysis of the DXDDTA motif in squalene-hopene cyclase by site-directed mutagenesis experiments: Initiation site of the polycyclization reaction and stabilization site of the carbocation intermediate of the initially cyclized A-ring

Bioscience, Biotechnology and Biochemistry

Volumn 63, Issue 12, 1999, Pages 2189-2198

  Sato, Tsutomu ^a   Hoshino, Tsutomu ^a  

^a NIIGATA UNIVERSITY   (Japan)

Author keywords

Alicyclobacillus acidocaldarius; DXDDTA motif; Hopene; Oxidosqualene; Squalene

Indexed keywords

MUTASE; SQUALENE HOPENE CYCLASE; SQUALENE-HOPENE CYCLASE;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ARTICLE; CHEMICAL MODEL; CHEMISTRY; CIRCULAR DICHROISM; GENETICS; MOLECULAR GENETICS; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; PROTEIN CONFORMATION; SITE DIRECTED MUTAGENESIS; STRUCTURE ACTIVITY RELATION; TEMPERATURE;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; CIRCULAR DICHROISM; INTRAMOLECULAR TRANSFERASES; MAGNETIC RESONANCE SPECTROSCOPY; MODELS, CHEMICAL; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; PROTEIN CONFORMATION; STRUCTURE-ACTIVITY RELATIONSHIP; TEMPERATURE;

EID: 0033252974     PISSN: 09168451     EISSN: 13476947     Source Type: Journal    
DOI: 10.1271/bbb.63.2189     Document Type: Article

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