Site-directed mutagenesis of putative active-site residues in squalene-hopene cyclase

European Journal of Biochemistry

Volumn 242, Issue 1, 1996, Pages 51-55

  Feil, Corinna ^a   Süssmuth, Roderich ^a   Jung, Günther ^a   Poralla, Karl ^a  

^a UNIVERSITY OF TÜBINGEN   (Germany)

Author keywords

Active site; Alicyclobacillus acidocaldarius; Circular dichroism; Mutagenesis; Squalene hopene cyclase

Indexed keywords

SYNTHETASE;

ARTICLE; BACILLUS; CIRCULAR DICHROISM; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME PURIFICATION; ENZYME STRUCTURE; ESCHERICHIA COLI; GENE EXPRESSION; PRIORITY JOURNAL; SEQUENCE ANALYSIS; SEQUENCE HOMOLOGY; SITE DIRECTED MUTAGENESIS; THERMOSTABILITY;

ALICYCLOBACILLUS ACIDOCALDARIUS; ESCHERICHIA COLI;

EID: 0029854776     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1432-1033.1996.0051r.x     Document Type: Article

References (24)

1. Sahm, H., Rohmer, M., Bringer-Meyer, S., Sprenger, G. A. & Welle, R. (1993) Biochemistry and physiology of hopanoids in bacteria, Adv. Microbial Physiol. 35, 247-273.
2. Ourisson, G., Rohmer, M. & Poralla, K. (1987) Prokaryotic hopanoids and other polyterpenoid sterol surrogates, Annu. Rev. Microbiol. 41, 301-333.
3. Corey, E. J., Matsuda, S. P. T. & Bartel, B. (1994) Molecular cloning, characterization, and overexpression of ERG7, the Saccharomyces cerevisiae gene encoding lanosterol synthase, Proc. Natl Acad. Sci. USA 91, 2211-2215.
4. Poralla, K., Hewelt, A., Prestwich, G. D., Abe, I., Reipen, I. & Sprenger, G. (1994) A specific amino acid repeat in squalene and oxidosqualene cyclases, Trends Biochem. Sci. 19, 157-158.
5. Poralla, K. (1994) The possible role of a repetitive amino acid motif in evolution of triterpenoid cyclases, Bioorg. Med. Chem. Lett. 4, 285-290.
6. Dougherty, D. A. (1996) Cation-π interactions in chemistry and biology: a new view of benzene. Phe, Tyr, and Trp, Science 271, 163-168.
7. Abe, I. & Prestwich, G. D. (1995) Molecular cloning, characterization, and functional expression of rat oxidosqualene cyclase cDNA, Proc. Natl Acad. Sci. USA 92, 9274-9278.
8. Abe, I. & Prestwich, G. D. (1994) Active site mapping of affinity labeled rat oxidosqualene cyclase, J. Biol. Chem. 269, 802-804.
9. Abe, I. & Prestwich, G. D. (1995) Identification of the active site of vertebrate oxidosqualene cyclase, Lipids 30, 231-234.
10. Benson, R. J., Johal, G. S., Crane, V. C., Tossberg, J. T., Schnable, P. S., Meeley, R. & Briggs, S. P. (1995) Cloning and characterization of the maize An1 gene, Plant Cell 7, 75-84.
11. Sun, T. & Kamiya, Y. (1994) The arabidopsis GA1 locus encodes the cyclase ent-kaurene synthetase A of gibberellin biosynthesis, Plant Cell 6, 1509-1518.
12. Sambrook, J., Fritsch, E. F. & Maniatis, T. (1989) Molecular cloning: a laboratory manual, 2nd edn, Cold Spring Harbor Laboratory, Cold Spring Harbor NY.
13. Sanger, F., Nicklen, S. & Coulson, A. R. (1977) DNA sequencing with chain-terminating inhibitors, Proc. Natl Acad. Sci. USA 74, 5463-5467.
14. Ochs, D., Kaletta, C., Entian, K.-D., Beck-Sickinger, A. & Poralla, K. (1992) Cloning, expression, and sequencing of squalene-hopene cyclase, a key enzyme intriterpenoid metabolism, J. Bacteriol. 174, 298-302.
15. Blum, H., Beier, H. & Gross, H. (1987) Improved silver staining of plant proteins, RNA, and DNA in polyacrylamide gels, Electrophoresis 8, 93-99.
16. Bradford, M. M. (1976) A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248-254.
17. Ochs, D., Tappe, C., Gärtner, P., Kellner, R. & Poralla, K. (1990) Properties of purified squalene-hopene cyclase from Bacillus acidocaldarius, Eur. J. Biochem. 194, 75-80.
18. Lineweaver, H. & Burk, D. (1934) The determination of enzyme dissociation constants, J. Am. Chem. Soc. 56, 658-666.
19. Rost, B. & Sander, C. (1994) Combining evolutionary information and neural networks to predict protein secondary structure. Proteins 19, 55-72.
20. Saar, J., Kader, J.-C., Poralla, K. & Ourisson, G. (1991) Purification and some properties of the squalene-tetrahymanol cyclase from Tetrahymena thermophila, Biochim. Biophvs. Acta 1075, 93-101.
21. Tappe, C. H. (1993) Squalen-Hopen Cyclasen: Reinigung. Charakterisierung und Inhibitor-Experimente, PhD thesis, University of Tübingen, Germany.
22. Seckler, B. & Poralla, K. (1986) Characterization and partial purification of the squalene-hopene cyclase from Bacillus acidocaldarius, Biochim. Biophys. Acta 220, 356-363.
23. Laemmli, U. K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4, Nature 227, 680-685.
24. Chang, C. T., Wu, C.-S. C. & Yang, J. T. (1978) Circular dichroic analysis of protein conformation: Inclusion of the β-turns, Anal. Biochem. 91, 13-31.