Overexpression of squalene-hopene cyclase by the pet vector in escherichia coli and first identification of tryptophan and aspartic acid residues inside the qw motif as active sites

Bioscience, Biotechnology and Biochemistry

Volumn 62, Issue 2, 1998, Pages 407-411

  Sato, Tsutomu ^a   Kanai, Yoshinori ^a   Hoshino, Tsutomu ^a  

^a NIIGATA UNIVERSITY   (Japan)

Author keywords

Alicyclobacillus acidocaldarius; Hopene; Site directed mutagenesis; Squalene; Squalene cyclase

Indexed keywords

ASPARTIC ACID; BACTERIAL DNA; DIPLOPTENE; ISOPROPYL THIOGALACTOSIDE; MUTASE; SQUALENE; SQUALENE HOPENE CYCLASE; SQUALENE-HOPENE CYCLASE; TRITERPENE; TRYPTOPHAN;

AMINO ACID SEQUENCE; ARTICLE; BACILLACEAE; BIOSYNTHESIS; CHEMISTRY; DNA SEQUENCE; ENZYMOLOGY; ESCHERICHIA COLI; GENE EXPRESSION REGULATION; GENE VECTOR; GENETIC TRANSFORMATION; GENETICS; KINETICS; MOLECULAR GENETICS; PLASMID; POLYACRYLAMIDE GEL ELECTROPHORESIS; SEQUENCE HOMOLOGY; SITE DIRECTED MUTAGENESIS;

AMINO ACID SEQUENCE; ASPARTIC ACID; BACILLACEAE; DNA, BACTERIAL; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; GENE EXPRESSION REGULATION, BACTERIAL; GENETIC VECTORS; INTRAMOLECULAR TRANSFERASES; ISOPROPYL THIOGALACTOSIDE; KINETICS; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; PLASMIDS; SEQUENCE ANALYSIS, DNA; SEQUENCE HOMOLOGY, AMINO ACID; SQUALENE; TRANSFORMATION, GENETIC; TRITERPENES; TRYPTOPHAN;

EID: 0031992366     PISSN: 09168451     EISSN: 13476947     Source Type: Journal    
DOI: 10.1271/bbb.62.407     Document Type: Article

References (23)

1. D. Ochs, C. Kaletta, K-D. Entian, A. Beck-Sickinger, and K. Poralla, J. Bacteriol., 174, 298-302 (1992).
2. I. G. Reipen, K. Poralla, H. Sahm, and G. A. Sprenger, Microbiology, 141, 155-161 (1995).
3. C. J. Buntei and J. H. Griffin, J. Am. Chem. Soc., 114, 9711— 9713 (1992).
4. C. A. Roessner, C. Min, S. H. Hardin, L. W. Harris-Haller, J. C. McCollum, and A. I. Scott, Gene, 127, 149-150 (1993).
5. E. J. Corey, S. P. T. Matsuda, and B. Bartel, Proc. Natl. Acad. Sei. USA., 91, 2211-2215 (1994).
6. Z. Shi, C. J. Buntei, and J. H. Griffin, Proc. Natl. Acad. Sei. USA, 91, 7370-7374 (1994).
7. E. J. Corey, S. P. T. Matsuda, C. H. Baker, A. Y. Ting, and H. Cheng, Bioehem. Biophys. Res. Commun., 219, 327-331 (1996).
8. E. J. Corey, S. P. T. Matsuda, and B. Bartel, Proc. Natl. Acad. Sei. USA, 90, 11628-11632 (1993).
9. M. Kusano, M. Shibuya, U. Sankawa, and Y. Ebizuka, Biol. Pharm. Bull., 18, 195-197 (1995).
10. I. Abe and G. D. Prestwich, Proc. Natl. Acad. Sei. USA, 92, 9274-9278 (1995).
11. C. K. Sung, M. Shibuya, U. Sankawa, and Y. Ebizuka, Biol. Pharm. Bull., 18, 1459-1461 (1995).
12. C. H. Baker, S. P. T. Matsuda, D. R. Liu, and E. J. Corey, Biochem. Biophys. Res. Commun., 213, 154-160 (1995).
13. W. S. Johnson, S. D. Lindeil, and J. Steele, J. Am. Chem. Soc., 109, 5852-5853 (1987).
14. K. Poralla, Bioorg. Med. Chem. Lett., 4, 285-290 (1994).
15. C. Feil, R. Sussmuth, G. Jung, and K. Poralla, Eur. J. Biochem., 242, 51-55 (1996).
16. A. H. Rosenberg, B. N. Lade, D. Chui, S-W. Lin, J. J. Dunn, and F. W. Studier, Gene, 56, 125-135 (1987).
17. G. Darland and T. D. Brock, J. Gen. Microbiol., 67, 9-15 (1971).
18. O. H. Lowry, N. J. Rosebrough, A. L. Farr, and R. J. Randall, J. Biol. Chem., 193, 265-275 (1951).
19. E. J. Corey, S. C. Virgil, H. Cheng, C. H. Baker, S. P. T. Matsuda, V. Singh, and S. Sarshar, J. Am. Chem. Soc., 117, 11819-11820 (1995).
20. T. Hoshino, E. Ishibashi, and K. Kaneko, J. Chem. Soc. Chem. Commun., 1995, 2401-2402.
21. M. Nishio and M. Hirota, Tetrahedron, 45, 7201-7245 (1989).
22. M. Nishio, Y. Umezawa, and M. Hirota, Kagaku to Seibutsu (in Japanese), 33, 311-318 (1995).
23. K. U. Wendt, K. Poralla, and G. E. Schulz, Science, 277, 1811-1815 (1997).