Methodology for the preparation of pure recombinant S. cerevisiae lanosterol synthase using a baculovirus expression system. Evidence that oxirane cleavage and A-ring formation are concerted in the biosynthesis of lanosterol from 2,3-oxidosqualene

Journal of the American Chemical Society

Volumn 119, Issue 6, 1997, Pages 1277-1288

  Corey, E J ^a   Cheng, Hengmiao ^a   Baker, C Hunter ^a   Matsuda, Seiichi P T ^a   Li, Ding ^a   Song, Xuelei ^a  

^a HARVARD UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

LANOSTEROL SYNTHASE; RECOMBINANT PROTEIN; VIRUS VECTOR;

ANIMAL CELL; ARTICLE; BACULOVIRUS; ENZYME ACTIVITY; ENZYME ANALYSIS; GENE EXPRESSION; INSECT; NONHUMAN; PROTEIN PURIFICATION; SACCHAROMYCES CEREVISIAE; SITE DIRECTED MUTAGENESIS;

EID: 0030934889     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja963227w     Document Type: Article

References (31)

1. For a recent review, see Abe, I.; Rohmer, M.; Prestwich, G. D. Chem. Rev. 1993, 93, 2189.
2. For recent work on the gene cloning and protein sequences for lanosterol synthase form various organisms, see: (a) Kelly, R.; Miller, S. M.; Lai, M. H.; Kirsch, D. R. Gene 1990, 87, 177 and Buntel, C. J.; Griffin, J. H. J. Am. Chem. Soc. 1992, 114, 9711 (Candida albicans). (b) Corey, E. J.; Matsuda, S. P. T.; Bartel, B. Proc. Natl. Acad. Sci. U.S.A. 1993, 90, 11628 (Arabidopsis thaliana). (c) Corey, E. J.; Matsuda, S. P. T.; Bartel, B. Proc. Natl. Acad. Sci. U.S.A. 1994, 91, 2211. Shi, Z.; Buntel, C. J.; Griffin, J. H.; Proc. Natl. Acad. Sci. U.S.A. 1994, 91, 7370 (Saccharomyces cerevisiae). (d) Kasuno, M.; Shibuya, M.; Sankawa, U.; Ebizuka, Y. Biol. Pharm. Bull. Jpn. 1995, 18, 195. Abe, I.; Prestwich, G. D. Proc. Natl. Acad. Sci. U.S.A. 1995, 92, 9274 (Rattus norvegicus). (e) Baker, C. H.; Matsuda, S. P. T.; Liu, D. R.; Corey, E. J. Biochem. Biophys. Res. Commun. 1995, 213, 154 (Homo sapiens). (f) Corey, E. J.; Matsuda, S. P. T.; Baker, C. H.; Ting, A.; Cheng, H. Biochem. Biophys. Res. Commun. 1996, 219, 327 (Schizosaccharomyces pombe).
3. For recent work on the gene cloning and protein sequences for lanosterol synthase form various organisms, see: (a) Kelly, R.; Miller, S. M.; Lai, M. H.; Kirsch, D. R. Gene 1990, 87, 177 and Buntel, C. J.; Griffin, J. H. J. Am. Chem. Soc. 1992, 114, 9711 (Candida albicans). (b) Corey, E. J.; Matsuda, S. P. T.; Bartel, B. Proc. Natl. Acad. Sci. U.S.A. 1993, 90, 11628 (Arabidopsis thaliana). (c) Corey, E. J.; Matsuda, S. P. T.; Bartel, B. Proc. Natl. Acad. Sci. U.S.A. 1994, 91, 2211. Shi, Z.; Buntel, C. J.; Griffin, J. H.; Proc. Natl. Acad. Sci. U.S.A. 1994, 91, 7370 (Saccharomyces cerevisiae). (d) Kasuno, M.; Shibuya, M.; Sankawa, U.; Ebizuka, Y. Biol. Pharm. Bull. Jpn. 1995, 18, 195. Abe, I.; Prestwich, G. D. Proc. Natl. Acad. Sci. U.S.A. 1995, 92, 9274 (Rattus norvegicus). (e) Baker, C. H.; Matsuda, S. P. T.; Liu, D. R.; Corey, E. J. Biochem. Biophys. Res. Commun. 1995, 213, 154 (Homo sapiens). (f) Corey, E. J.; Matsuda, S. P. T.; Baker, C. H.; Ting, A.; Cheng, H. Biochem. Biophys. Res. Commun. 1996, 219, 327 (Schizosaccharomyces pombe).
4. For recent work on the gene cloning and protein sequences for lanosterol synthase form various organisms, see: (a) Kelly, R.; Miller, S. M.; Lai, M. H.; Kirsch, D. R. Gene 1990, 87, 177 and Buntel, C. J.; Griffin, J. H. J. Am. Chem. Soc. 1992, 114, 9711 (Candida albicans). (b) Corey, E. J.; Matsuda, S. P. T.; Bartel, B. Proc. Natl. Acad. Sci. U.S.A. 1993, 90, 11628 (Arabidopsis thaliana). (c) Corey, E. J.; Matsuda, S. P. T.; Bartel, B. Proc. Natl. Acad. Sci. U.S.A. 1994, 91, 2211. Shi, Z.; Buntel, C. J.; Griffin, J. H.; Proc. Natl. Acad. Sci. U.S.A. 1994, 91, 7370 (Saccharomyces cerevisiae). (d) Kasuno, M.; Shibuya, M.; Sankawa, U.; Ebizuka, Y. Biol. Pharm. Bull. Jpn. 1995, 18, 195. Abe, I.; Prestwich, G. D. Proc. Natl. Acad. Sci. U.S.A. 1995, 92, 9274 (Rattus norvegicus). (e) Baker, C. H.; Matsuda, S. P. T.; Liu, D. R.; Corey, E. J. Biochem. Biophys. Res. Commun. 1995, 213, 154 (Homo sapiens). (f) Corey, E. J.; Matsuda, S. P. T.; Baker, C. H.; Ting, A.; Cheng, H. Biochem. Biophys. Res. Commun. 1996, 219, 327 (Schizosaccharomyces pombe).
5. For recent work on the gene cloning and protein sequences for lanosterol synthase form various organisms, see: (a) Kelly, R.; Miller, S. M.; Lai, M. H.; Kirsch, D. R. Gene 1990, 87, 177 and Buntel, C. J.; Griffin, J. H. J. Am. Chem. Soc. 1992, 114, 9711 (Candida albicans). (b) Corey, E. J.; Matsuda, S. P. T.; Bartel, B. Proc. Natl. Acad. Sci. U.S.A. 1993, 90, 11628 (Arabidopsis thaliana). (c) Corey, E. J.; Matsuda, S. P. T.; Bartel, B. Proc. Natl. Acad. Sci. U.S.A. 1994, 91, 2211. Shi, Z.; Buntel, C. J.; Griffin, J. H.; Proc. Natl. Acad. Sci. U.S.A. 1994, 91, 7370 (Saccharomyces cerevisiae). (d) Kasuno, M.; Shibuya, M.; Sankawa, U.; Ebizuka, Y. Biol. Pharm. Bull. Jpn. 1995, 18, 195. Abe, I.; Prestwich, G. D. Proc. Natl. Acad. Sci. U.S.A. 1995, 92, 9274 (Rattus norvegicus). (e) Baker, C. H.; Matsuda, S. P. T.; Liu, D. R.; Corey, E. J. Biochem. Biophys. Res. Commun. 1995, 213, 154 (Homo sapiens). (f) Corey, E. J.; Matsuda, S. P. T.; Baker, C. H.; Ting, A.; Cheng, H. Biochem. Biophys. Res. Commun. 1996, 219, 327 (Schizosaccharomyces pombe).
6. For recent work on the gene cloning and protein sequences for lanosterol synthase form various organisms, see: (a) Kelly, R.; Miller, S. M.; Lai, M. H.; Kirsch, D. R. Gene 1990, 87, 177 and Buntel, C. J.; Griffin, J. H. J. Am. Chem. Soc. 1992, 114, 9711 (Candida albicans). (b) Corey, E. J.; Matsuda, S. P. T.; Bartel, B. Proc. Natl. Acad. Sci. U.S.A. 1993, 90, 11628 (Arabidopsis thaliana). (c) Corey, E. J.; Matsuda, S. P. T.; Bartel, B. Proc. Natl. Acad. Sci. U.S.A. 1994, 91, 2211. Shi, Z.; Buntel, C. J.; Griffin, J. H.; Proc. Natl. Acad. Sci. U.S.A. 1994, 91, 7370 (Saccharomyces cerevisiae). (d) Kasuno, M.; Shibuya, M.; Sankawa, U.; Ebizuka, Y. Biol. Pharm. Bull. Jpn. 1995, 18, 195. Abe, I.; Prestwich, G. D. Proc. Natl. Acad. Sci. U.S.A. 1995, 92, 9274 (Rattus norvegicus). (e) Baker, C. H.; Matsuda, S. P. T.; Liu, D. R.; Corey, E. J. Biochem. Biophys. Res. Commun. 1995, 213, 154 (Homo sapiens). (f) Corey, E. J.; Matsuda, S. P. T.; Baker, C. H.; Ting, A.; Cheng, H. Biochem. Biophys. Res. Commun. 1996, 219, 327 (Schizosaccharomyces pombe).
7. For recent work on the gene cloning and protein sequences for lanosterol synthase form various organisms, see: (a) Kelly, R.; Miller, S. M.; Lai, M. H.; Kirsch, D. R. Gene 1990, 87, 177 and Buntel, C. J.; Griffin, J. H. J. Am. Chem. Soc. 1992, 114, 9711 (Candida albicans). (b) Corey, E. J.; Matsuda, S. P. T.; Bartel, B. Proc. Natl. Acad. Sci. U.S.A. 1993, 90, 11628 (Arabidopsis thaliana). (c) Corey, E. J.; Matsuda, S. P. T.; Bartel, B. Proc. Natl. Acad. Sci. U.S.A. 1994, 91, 2211. Shi, Z.; Buntel, C. J.; Griffin, J. H.; Proc. Natl. Acad. Sci. U.S.A. 1994, 91, 7370 (Saccharomyces cerevisiae). (d) Kasuno, M.; Shibuya, M.; Sankawa, U.; Ebizuka, Y. Biol. Pharm. Bull. Jpn. 1995, 18, 195. Abe, I.; Prestwich, G. D. Proc. Natl. Acad. Sci. U.S.A. 1995, 92, 9274 (Rattus norvegicus). (e) Baker, C. H.; Matsuda, S. P. T.; Liu, D. R.; Corey, E. J. Biochem. Biophys. Res. Commun. 1995, 213, 154 (Homo sapiens). (f) Corey, E. J.; Matsuda, S. P. T.; Baker, C. H.; Ting, A.; Cheng, H. Biochem. Biophys. Res. Commun. 1996, 219, 327 (Schizosaccharomyces pombe).
8. For recent work on the gene cloning and protein sequences for lanosterol synthase form various organisms, see: (a) Kelly, R.; Miller, S. M.; Lai, M. H.; Kirsch, D. R. Gene 1990, 87, 177 and Buntel, C. J.; Griffin, J. H. J. Am. Chem. Soc. 1992, 114, 9711 (Candida albicans). (b) Corey, E. J.; Matsuda, S. P. T.; Bartel, B. Proc. Natl. Acad. Sci. U.S.A. 1993, 90, 11628 (Arabidopsis thaliana). (c) Corey, E. J.; Matsuda, S. P. T.; Bartel, B. Proc. Natl. Acad. Sci. U.S.A. 1994, 91, 2211. Shi, Z.; Buntel, C. J.; Griffin, J. H.; Proc. Natl. Acad. Sci. U.S.A. 1994, 91, 7370 (Saccharomyces cerevisiae). (d) Kasuno, M.; Shibuya, M.; Sankawa, U.; Ebizuka, Y. Biol. Pharm. Bull. Jpn. 1995, 18, 195. Abe, I.; Prestwich, G. D. Proc. Natl. Acad. Sci. U.S.A. 1995, 92, 9274 (Rattus norvegicus). (e) Baker, C. H.; Matsuda, S. P. T.; Liu, D. R.; Corey, E. J. Biochem. Biophys. Res. Commun. 1995, 213, 154 (Homo sapiens). (f) Corey, E. J.; Matsuda, S. P. T.; Baker, C. H.; Ting, A.; Cheng, H. Biochem. Biophys. Res. Commun. 1996, 219, 327 (Schizosaccharomyces pombe).
9. For recent work on the gene cloning and protein sequences for lanosterol synthase form various organisms, see: (a) Kelly, R.; Miller, S. M.; Lai, M. H.; Kirsch, D. R. Gene 1990, 87, 177 and Buntel, C. J.; Griffin, J. H. J. Am. Chem. Soc. 1992, 114, 9711 (Candida albicans). (b) Corey, E. J.; Matsuda, S. P. T.; Bartel, B. Proc. Natl. Acad. Sci. U.S.A. 1993, 90, 11628 (Arabidopsis thaliana). (c) Corey, E. J.; Matsuda, S. P. T.; Bartel, B. Proc. Natl. Acad. Sci. U.S.A. 1994, 91, 2211. Shi, Z.; Buntel, C. J.; Griffin, J. H.; Proc. Natl. Acad. Sci. U.S.A. 1994, 91, 7370 (Saccharomyces cerevisiae). (d) Kasuno, M.; Shibuya, M.; Sankawa, U.; Ebizuka, Y. Biol. Pharm. Bull. Jpn. 1995, 18, 195. Abe, I.; Prestwich, G. D. Proc. Natl. Acad. Sci. U.S.A. 1995, 92, 9274 (Rattus norvegicus). (e) Baker, C. H.; Matsuda, S. P. T.; Liu, D. R.; Corey, E. J. Biochem. Biophys. Res. Commun. 1995, 213, 154 (Homo sapiens). (f) Corey, E. J.; Matsuda, S. P. T.; Baker, C. H.; Ting, A.; Cheng, H. Biochem. Biophys. Res. Commun. 1996, 219, 327 (Schizosaccharomyces pombe).
10. For recent work on the gene cloning and protein sequences for lanosterol synthase form various organisms, see: (a) Kelly, R.; Miller, S. M.; Lai, M. H.; Kirsch, D. R. Gene 1990, 87, 177 and Buntel, C. J.; Griffin, J. H. J. Am. Chem. Soc. 1992, 114, 9711 (Candida albicans). (b) Corey, E. J.; Matsuda, S. P. T.; Bartel, B. Proc. Natl. Acad. Sci. U.S.A. 1993, 90, 11628 (Arabidopsis thaliana). (c) Corey, E. J.; Matsuda, S. P. T.; Bartel, B. Proc. Natl. Acad. Sci. U.S.A. 1994, 91, 2211. Shi, Z.; Buntel, C. J.; Griffin, J. H.; Proc. Natl. Acad. Sci. U.S.A. 1994, 91, 7370 (Saccharomyces cerevisiae). (d) Kasuno, M.; Shibuya, M.; Sankawa, U.; Ebizuka, Y. Biol. Pharm. Bull. Jpn. 1995, 18, 195. Abe, I.; Prestwich, G. D. Proc. Natl. Acad. Sci. U.S.A. 1995, 92, 9274 (Rattus norvegicus). (e) Baker, C. H.; Matsuda, S. P. T.; Liu, D. R.; Corey, E. J. Biochem. Biophys. Res. Commun. 1995, 213, 154 (Homo sapiens). (f) Corey, E. J.; Matsuda, S. P. T.; Baker, C. H.; Ting, A.; Cheng, H. Biochem. Biophys. Res. Commun. 1996, 219, 327 (Schizosaccharomyces pombe).
11. Vectors used: pKK223-3, pLM1, pKen2, pET-15b. Strains used: JM101, XA90, BL21-DE(3), BL21-DE(3)pLysS.
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