New Cyclization Mechanism for Squalene: A Ring-expansion Step for the Five-membered C-ring Intermediate in Hopene Biosynthesis

Bioscience, Biotechnology and Biochemistry

Volumn 63, Issue 11, 1999, Pages 2038-2041

  Hoshino, Tsutomu ^a   Kouda, Masanori ^a   Abe, Takamasa ^a   Ohashi, Shumi ^a  

^a NIIGATA UNIVERSITY   (Japan)

Author keywords

2,3 oxidosqualene; Hopene; Squalene; Triterpene cyclase

Indexed keywords

DIPLOPTENE; MUTASE; RECOMBINANT PROTEIN; SQUALENE; SQUALENE HOPENE CYCLASE; SQUALENE-HOPENE CYCLASE; TRITERPENE;

AMINO ACID SUBSTITUTION; ARTICLE; CHEMICAL STRUCTURE; CHEMISTRY; METABOLISM; SITE DIRECTED MUTAGENESIS; SYNTHESIS;

AMINO ACID SUBSTITUTION; INTRAMOLECULAR TRANSFERASES; MODELS, MOLECULAR; MOLECULAR STRUCTURE; MUTAGENESIS, SITE-DIRECTED; RECOMBINANT PROTEINS; SQUALENE; TRITERPENES;

EID: 0033218286     PISSN: 09168451     EISSN: 13476947     Source Type: Journal    
DOI: 10.1271/bbb.63.2038     Document Type: Article

References (17)

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14. The structures of tricyclic products 8 and 9 have not been determined in ref. 13. We have independently reported the function of Phe601, described here, at the annual meeting of Japan Society for Biosci., Biotechnol., and Agrochem. March, 1999, Fukuoka, Japan. Abstract p. 306. The GC pattern of mutant F601A, which was constructed by us, was the same as that in the ref. 13.
15. c (ppm), C-8 (44.38), C-9 (59.61), C-10 (37.45), C-12 (25.19), C-13 (60.12), C-14 (85.35), C-15 (38.30), C-16 (26.63), C-17 (65.98), C-21 (26.63), C-22 (24.50). No NOE between H-13 and H-9 suggests the endo-orientation for the THF moiety.
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