Trichodiene synthase. Enzymatic formation of multiple sesquiterpenes by alteration of the cyclase active site

Journal of the American Chemical Society

Volumn 118, Issue 6, 1996, Pages 1563-1564

  Cane, David E ^a   Xue, Qun ^a  

^a BROWN UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CYCLASE; SESQUITERPENE; UNCLASSIFIED DRUG;

ARTICLE; DRUG SYNTHESIS; ENZYME ACTIVE SITE; ENZYME BINDING; FUSARIUM; GAS CHROMATOGRAPHY; MASS SPECTROMETRY; NUCLEAR MAGNETIC RESONANCE; STEREOCHEMISTRY;

EID: 0029928923     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja953751n     Document Type: Article

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28. 9,11 for 3 h at 30°C. The hydrocarbon products were extracted with HPLC grade pentane and purified on silica gel columns (3 cm) packed in Pasteur pipets overlaid with sodium sulfate (0.5 cm). The extracts of each incubation were concentrated in vacuo at 0°C. and the concentrates (5 μL) were analyzed by GC-EI/MS.
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