Functional analysis of phenylalanine 365 in hopene synthase, a conserved amino acid in the families of squalene and oxidosqualene cyclases

Chemical Communications

Volumn , Issue 19, 1999, Pages 2005-2006

  Hoshino, Tsutomu ^a   Sato, Tsutomu ^a  

^a NIIGATA UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

LANOSTEROL SYNTHASE; PHENYLALANINE;

ARTICLE; CYCLIZATION; LOW TEMPERATURE;

EID: 0033533760     PISSN: 13597345     EISSN: None     Source Type: Journal    
DOI: 10.1039/a905559b     Document Type: Article

References (22)

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15. Analyses of NMR data (H-H COSY 45, HOHAHA, NOESY, DEPT, HMQC and HMBC) unequivocally supported the structures proposed for 4 and 5.
16. The temperatures for the Arrhenius plots for 1 were in the range of 30-60 and 10-25 °C for the wild-type and for the F365Y, respectively, while in the case of 6 they were 40-60, 30-50 and 20-30 °C, respectively, for the wild-type, the F365Y and the F365W.
17. 11 The denaturation process was confirmed to be reversible; the F365Y was exposed at 40 °C for 60 min and then incubated with 1 at 20 °C, but showed the same activity as that without such treatment. To validate our hypothesis, further evidence is required.
18. 6c
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