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7
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0031152073
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1997; 1:72-78. An excellent review of new technologies for high-throughput screening. The paper contains references on nonradioactive assay technologies, screening methods for combinatorial libraries, and issues associated with assay miniaturization.
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6.Burbaum JJ, Sigal NH: New technologies for high-throughput screening. Curr Opin Chem Biol 1997; 1:72-78. An excellent review of new technologies for high-throughput screening. The paper contains references on nonradioactive assay technologies, screening methods for combinatorial libraries, and issues associated with assay miniaturization.
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Sigal NH: New Technologies for High-throughput Screening. Curr Opin Chem Biol
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Burbaum, J.J.1
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8
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0001180917
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1997;2:159-169. This paper describes the concept of signal windows, which provides a degree of separation between measured signals. The size of the signal window is a critical performance parameter (in high-throughput screens) which impacts the identification of active compounds (hits) in the presence of varibility.
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l: Sittampalam GS, Iversen PW, Boadt JA, Kahl SD, Bright S, Zock JM, Janzen WP, Lister MD: Design of signal windows in high throughput screening assays for drug discovery. J Biomol Screening 1997;2:159-169. This paper describes the concept of signal windows, which provides a degree of separation between measured signals. The size of the signal window is a critical performance parameter (in high-throughput screens) which impacts the identification of active compounds (hits) in the presence of varibility.
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Iversen PW, Boadt JA, Kahl SD, Bright S, Zock JM, Janzen WP, Lister MD: Design of Signal Windows in High Throughput Screening Assays for Drug Discovery. J Biomol Screening
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Sittampalam, G.S.1
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12
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0000885268
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1:287-294. A good account of the basic principles of the scintillation proximity assay and its specific applications in high-throughput screening, with 70 references cited.
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11.' Cook ND: Scintillation proximity assay: a versatile high throughput screening technology. Drug Discov Tech 1996,1:287-294. A good account of the basic principles of the scintillation proximity assay and its specific applications in high-throughput screening, with 70 references cited.
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Scintillation Proximity Assay: A Versatile High Throughput Screening Technology. Drug Discov Tech 1996
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Cook, N.D.1
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14
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33748073283
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This paper describes the development of a scintillation proximity assay for receptor binding studies in high-throughput mode. Validation concepts and factors affecting robustness of the assays in highthroughput screening mode are addressed in detail.
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Concepts, T.P.1
Detail, F.A.2
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16
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0030586294
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1996;239:20-24.
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BakerCA,PoormanRA,KezdyFJ,StaplesDJ,SmithCW,Elhammer AP: A scintillation proximity assay for UDP-GaINAc:polypeptide, NAcetylgalactosaminyltransferase. Anal Biochem 1996;239:20-24.
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KezdyFJ,StaplesDJ,SmithCW,Elhammer AP: A Scintillation Proximity Assay for UDP-GaINAc:polypeptide, NAcetylgalactosaminyltransferase. Anal Biochem
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Baker, C.A.1
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Sonatore LM, Wisniewski D, Frank LJ, Cameron PM, Hermes JD, Marcy Al, Salowe SP: The utility of FK506-binding protein as a fusion partner in scintillation proximity assays: application to SH2 domains. Anal Biochem 1996;240:289-297.
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Wisniewski D, Frank LJ, Cameron PM, Hermes JD, Marcy Al, Salowe SP: the Utility of FK
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2 domains of p72Sk. J Biol Chem 1996;271:25308-25315.
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Chen T, Repetto B, Chizzonite R, Pullar C, Burghardt C, Dharm E, Zhao Z, Carroll R, Nunes P, Basu M, et al: Interaction of phosphorylated FceRly immunoglobulin receptor tyrosine activation motifbased peptides with dual and single SH2 domains of p72S)k. J Biol Chem 1996;271:25308-25315.
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Repetto B, Chizzonite R, Pullar C, Burghardt C, Dharm E, Zhao Z, Carroll R, Nunes P, Basu M, et Al: Interaction of Phosphorylated FceRly Immunoglobulin Receptor Tyrosine Activation Motifbased Peptides with Dual and Single SH
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Chen, T.1
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27
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33748083848
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1997, 94:4-8. A good review of the fundamentals of fluorescence techniques for biological measurements. Detection techniques described are easily amenable to high-throughput screening assays. A section on recent advances describes developments in fluorescence resonance energy transfer (FRET) and fluorescence in situ hybridization (FISH) technologies, membrane potential measurements, and karyotyping human chromosomes. Over 40 references are cited on various applications.
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24.' Glazer AN : Recent advances in fluorescence labeling, detection and visualization. BioRadiations 1997, 94:4-8. A good review of the fundamentals of fluorescence techniques for biological measurements. Detection techniques described are easily amenable to high-throughput screening assays. A section on recent advances describes developments in fluorescence resonance energy transfer (FRET) and fluorescence in situ hybridization (FISH) technologies, membrane potential measurements, and karyotyping human chromosomes. Over 40 references are cited on various applications.
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Recent Advances in Fluorescence Labeling, Detection and Visualization. BioRadiations
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Glazer, A.N.1
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42
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0031016380
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Method to Analyze Drug/target Interactions. J Recept Signal Transduct Res 1997; 17:511-520. This Paper Describes the Use of Fluorescence Correlation Spectroscopy to Measure Molecular Interactions in A Homogeneous Mode. It Has Been Developed to Accommodate Measurements in 96- and 384well Microtiter Plates, and Is A Good Candidate for High-throughput Screening Applications
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39.' S terrer S, Henco K: Fluorescence correlation spectroscopy (PCS)A highly sensitive method to analyze drug/target interactions. J Recept Signal Transduct Res 1997; 17:511-520. This paper describes the use of fluorescence correlation spectroscopy to measure molecular interactions in a homogeneous mode. It has been developed to accommodate measurements in 96- and 384well microtiter plates, and is a good candidate for high-throughput screening applications.
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Fluorescence Correlation Spectroscopy (PCS)A Highly Sensitive
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Sterrer, S.1
Henco, K.2
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Sarubbi E, Yanofsky SD, Barren RW, Denaro M: A cell-free, nonisotopic, high-throughput assay for inhibitors of type-1 interleukin-1 receptor. Anal Biochem 1996;237:70-75.
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Yanofsky SD, Barren RW, Denaro M: A Cell-free, Nonisotopic, High-throughput Assay for Inhibitors of Type
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1996; 6:183-188.
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Rizzuto R, Brini M, De Giorgi F, Rossi R, Heim R, Tsien RY, Pozzan T: Double labelling subcellular structures with organelletargeted GFP mutants in vivo. Curr Biol 1996; 6:183-188.
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Brini M, de Giorgi F, Rossi R, Heim R, Tsien RY, Pozzan T: Double Labelling Subcellular Structures with Organelletargeted GFP Mutants in Vivo. Curr Biol
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Janzen B, Domanico P: The 384-well plate: pros and cons. J Biomol Screening 1996;l:63-64.
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1996; 1:277-286.
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Lutz MW, Menius A, Choi TD, Laskody RG, Domanico PL, Goetz AS, Saussy DL: Experimental design for high-throughput screening. Drug Discov Tech 1996; 1:277-286.
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Menius A, Choi TD, Laskody RG, Domanico PL, Goetz AS, Saussy DL: Experimental Design for High-throughput Screening. Drug Discov Tech
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1996; 3:113-120. A unique, membrane-based colorimetric system that detects molecular interactions is described. Gangliosides that specifically bind cholera toxin, Escherichia coli enterotoxin and botulism neurotoxin were incorporated into a polydiacetylene membrane. The polymer-ized membrane containing gangliosides is blue, and turns red when the toxin is added. The response is sensitive, specific and selective. An excellent technology for high-throughput screening applications.
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57. Charych D, Cheng Q, Reichert A, Kuziemko G, Stroh M, Nagy JO, Spevak W, Stevens RC: A 'litmus test' for molecular recognition using artificial membranes. Chem Biol 1996; 3:113-120. A unique, membrane-based colorimetric system that detects molecular interactions is described. Gangliosides that specifically bind cholera toxin, Escherichia coli enterotoxin and botulism neurotoxin were incorporated into a polydiacetylene membrane. The polymer-ized membrane containing gangliosides is blue, and turns red when the toxin is added. The response is sensitive, specific and selective. An excellent technology for high-throughput screening applications.
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Cheng Q, Reichert A, Kuziemko G, Stroh M, Nagy JO, Spevak W, Stevens RC: A 'Litmus Test' for Molecular Recognition Using Artificial Membranes. Chem Biol
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Brecht A, Burckardt R, Ricke J, Stemmler I, Schuetz A, Fischer S, Friedrich T, Gauglitz G, Goepel W: Transducer-based approaches for parallel binding assays in HTS. J Biomol Screening 1996; 1:191 -201.
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Burckardt R, Ricke J, Stemmler I, Schuetz A, Fischer S, Friedrich T, Gauglitz G, Goepel W: Transducer-based Approaches for Parallel Binding Assays in HTS. J Biomol Screening
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Heller RA, Schena M, Chai A, Shalon D, Bedilion T, Gilmore J, Woolley DE, Davis RW: Discovery and analysis of inflammatory disease-related genes using cDNA microarrays. Proc Nail Acad Sei USA 1997;94:2150-2155.
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Schena M, Chai A, Shalon D, Bedilion T, Gilmore J, Woolley DE, Davis RW: Discovery and Analysis of Inflammatory Disease-related Genes Using CDNA Microarrays. Proc Nail Acad Sei USA
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Evans DM, Williams KP, McGuinness B, Tarr G, Régnier F, Afeyan N, Jindal S: Affinity-based screening of combinatorial libraries using automated, serial-column chromatography. Nat Bioteclwl 1996;14:504-507.
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Williams KP, McGuinness B, Tarr G, Régnier F, Afeyan N, Jindal S: Affinity-based Screening of Combinatorial Libraries Using Automated, Serial-column Chromatography. Nat Bioteclwl
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