Development of a scintillation proximity assay for calcineurin phosphatase activity

Journal of Biomolecular Screening

Volumn 2, Issue 1, 1997, Pages 19-23

  Sullivan, Elaine ^a   Hemsley, Paul ^a   Pickard, Anne ^a  

^a ASTRAZENECA   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 0242445620     PISSN: 10870571     EISSN: None     Source Type: Journal    
DOI: 10.1177/108705719700200105     Document Type: Article

References (6)

1. Liu, J., Farmer, J.D., Lane, W.S., Friedman, J., Weissman, I., Schreiber, S. (1991). Calcineurin is a common target of cyclophilin-cyclosporin A and FKBP-FK506 complexes. Cell 66:807.
2. O'Keefe, S J., Tamura, J., Kincaid, R.L., Tocci, M., O'Neill, E.A. (1992). FK-506 and CsA-sensitive activation of the interleukin-2 promoter by calcineurin. Nature (London) 357:692.
3. Blumenthal, O.K., Takio, K., Hansen, R.S., Krebs, E.G. (1986). Dephosphorylation of cAMP-dependent protein kinase regulatory subunit (type II) by calmodulin-dependent protein phosphatase. J. Biol. Chem. 261:8140.
4. Perrino, B.A., Fong, Y.-L., Brickey, D.A., Saitoh, Y., Ushio, Y., Fukunaga, K., Miyamoto, E., Soderling, T.R. (1992). Characterisation of the phosphatase activity of a baculovirus-expressed calcineurin A isoform. J. Biol. Chem. 267:15965.
5. Enz, A., Shapiro, G., Chappuis, A., Dattier, A., (1994). Nonradioactive assay for protein phosphatase 2B (calcineurin) activity using a partial sequence of the subunit of cAMP-dependent protein kinase as substrate. Anal. Biochem. 216:147.
6. Cook, N.D. (1996). Scintillation proximity assay: A versatile high-throughput cpscreening technology. DDT 1:287.