Affinity-based screening of combinatorial libraries using automated, serial-column chromatography

Nature Biotechnology

Volumn 14, Issue 4, 1996, Pages 504-507

  Evans, David M ^a   Williams, Kevin P ^a   McGuinness, Brian ^a   Tarr, George ^a   Regnier, Fred ^a   Afeyan, Noubar ^a   Jindal, Satish ^a  

^a PERKINELMER INC   (United States)

Author keywords

Combinatorial libraries; Endotoxin; Molecular diversity; Screening; endorphin

Indexed keywords

COLUMN CHROMATOGRAPHY; COMBINATORIAL LIBRARY; LIBRARY; SCREENING; ARTICLE; AUTOMATION; BIOTECHNOLOGY; CHEMICAL ANALYSIS; PEPTIDE ANALYSIS; PRIORITY JOURNAL;

MONOCLONAL ANTIBODY;

AMINO ACID SEQUENCE; ANIMALS; ANTIBODIES, MONOCLONAL; BETA-ENDORPHIN; BIOTECHNOLOGY; CHROMATOGRAPHY, AFFINITY; DRUG DESIGN; HUMANS; LIPOPOLYSACCHARIDES; MICE; OLIGOPEPTIDES; PEPTIDE LIBRARY;

BACTERIA (MICROORGANISMS);

EID: 0029670582     PISSN: 10870156     EISSN: None     Source Type: Journal    
DOI: 10.1038/nbt0496-504     Document Type: Article

References (21)

1. Ecker, D J. and Crooke, S. T 1995. Combinatorial drug discovery: which methods will produce the greatest value? Bio/Technology 13:351-358.
2. Gordon, M., Barrett, R. W., Dower, W. J., Fodor, S. P. A., and Gallop, M. A. 1994. Applications of combinatorial technologies to drug discovery. 1. Background and peptide combinatorial libraries J. Med. Chem. 37:1233-1251.
3. Gordon, M., Barrett, R W., Dower, W. J., Fodor, S. P. A., and Gallop, M. A. 1994. Applications of combinatorial technologies to drug discovery. 2. Combinatonal organic synthesis, library screening strategies and future directions. J. Med. Chem. 37:1387-1401.
4. Lam, K. S., Salmon, S E. , Hersh, E. M., Hurby, V. J., Kazmierski, W. M., and Knapp, R. L. A new type of synthetic peptide library for identifying ligand-binding activity. Nature 354:82-84.
5. Houghten, R. A., Pinilla, C., Blondelle, S. E., Appel, J. R., Dooley, C. T., and Cuervo, J. H. 1991. Generation and use of synthetic peptide combinatorial libraries for basic research and drug discovery. Nature 354:84-86.
6. Scott, J. K. and Craig, L. 1994. Random peptide libraries. Curr. Biol. 5:40-48
7. Dooley, C. T., Chung, N. N., Schiller, P. W., and Houghten, R. A. 1993. Acetalins: Opioid receptor antagonists determined through the use of combinatorial libraries Proc. Natl. Acad. Sci. USA 90:10811-10815.
8. Dooley, C. T., Chung, N. N., Wilkes, B. C., Schiller, P. W., Bidlack, J M., Pasternak, G. W., and Houghten, R. A 1994. An all D-amino acid opioid peptide with central analgesic activity from a combinatorial library. Science 266:2019-2021
9. Houghten, R. A., Appel, J., Blondelle, S , Cuervo, J H , Dooley, C. T., and Pinilla, C. 1992 The use of synthetic peptide combinatorial libraries for the identification of bioactive peptides. Pep. Res. 5:351-358.
10. Lebl, M., Krchnak, V., Sepetov, N F., Seligmann, B., Strop, P., and Felder, S. 1995. One-bead-one structure combinatorial libraries. Biopolymers (Peptide Sci.) 37:177-198
11. Pinilla, C., Appel, J., Blondelle, S., Dooley, C., Dorner, B., Eichler, J., Ostresh, J., and Houghten, R A. 1995. A review of the utility of soluble peptide combinatorial libraries. Biopolymers (Peptide Sci ) 37:221-240.
12. Quesnel, A., Casrouge, A., Kourlisky, P., Abastado, J. P., and Trudelle, Y. 1995 Use of synthetic peptide libraries for the H-2Kd binding motif identification. Pep. Res. 8:44-51.
13. Flynn, G., Pohl, J., Flocco, M. T., and Rothman, J. E. 1991. Peptide-binding specificity of the molecular chaperone BiP. Nature 353:726-730.
14. Songyang, S., Shoelson, S. E., Chaudhn, M., Gish, G., Pawson, T., Haser, W. G., King, F., Roberts, T., Ratnofsky, S., Lechleider, B. G., Neel, R. B., Birge, J. E., Fajardo, M. M., Chou, H., Hanafusa, B., Schaffausen, B., and Cantley, L C. 1993 SH2 domains recognize specific phosphopeptide sequences. Cell 72:767-778.
15. Zuckermann, R. N., Kerr, J. M., Saini, M. A., Banville, S. C., and Santi, D. V. 1992. Identification of highest affinity ligands by affinity selection from equimolar peptide mixtures generated by robotic synthesis Proc. Natl. Acad. Sci. USA 83:4505-4509.
16. Evans, D. M., Williams, K. P., Parsons, G., and Jindal, S. 1995. A tandem column chromatographic method for studying the interaction between ligands and their targets lipopolysachharide as a model. Anal. Biochem 229:42-47
17. Afeyan, N. B., Gordon, N. F, Mazaroff, I . Varady, L., and Fulton, S. P. 1990. Flow through particles for the high-performance liquid chromatographic separation of biomolecules. Perfusion chromatography. J. Chromat. 519:1-29.
18. Afeyan, N. B. Fulton, S P., and Regnier, F. E 1991. High throughput chromatography using perfusive supports. LC GC 9:824-832.
19. Rustici, A., Velucchi, M., Faggioni, R., Sironi, M., Ghezzi, P, Quatert, S., Geen, B., and Porro, M. 1993. Molecular mapping and detoxification of the lipid A binding site by synthetic peptides. Science 259:361-365.
20. 1-5 J. Org Chem. 57:5399-5403.
21. Sebestyen, F., Dibo, G., Kovacs, A., and Furka, A. 1993. Chemical synthesis of peptide libraries. Bioorganic Med Chem. Letts. 3:413-418.