Fluorescence polarization analysis of protein-DNA and protein-protein interactions

Molecular Endocrinology

Volumn 10, Issue 6, 1996, Pages 607-612

  Lundblad, James R ^a   Laurance, Megan ^a   Goodman, Richard H ^a  

^a OREGON HEALTH AND SCIENCE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ALCOHOL; BUFFER; COUMARIN; CYCLIC AMP DEPENDENT PROTEIN KINASE; CYCLIC AMP RESPONSIVE ELEMENT BINDING PROTEIN; DIVALENT CATION; DNA; FLUORESCEIN; GLUCOCORTICOID RECEPTOR; OLIGONUCLEOTIDE; POLYNUCLEOTIDE; PROTEIN; RNA POLYMERASE; TRANSCRIPTION FACTOR;

ANISOTROPY; BINDING AFFINITY; ELECTROPHORETIC MOBILITY; EQUILIBRIUM DIALYSIS; FLUORESCENCE POLARIZATION; GENETIC TRANSCRIPTION; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRECIPITATION; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN DNA INTERACTION; PROTEIN PROTEIN INTERACTION; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; ROTATION; SHORT SURVEY;

CARRIER PROTEINS; CYCLIC AMP RESPONSE ELEMENT-BINDING PROTEIN; DNA; FLUORESCENCE POLARIZATION; MODELS, CHEMICAL; MODELS, MOLECULAR; PROTEINS;

EID: 0029887308     PISSN: 08888809     EISSN: None     Source Type: Journal    
DOI: 10.1210/me.10.6.607     Document Type: Short Survey

References (13)

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2. Lundblad JR, Kwok RP, Laurance ME, Harter ML, Goodman RH 1995 Adenoviral E1A-associated protein p300 as a functional homologue of the transcriptional co-activator CBP. Nature 374:85-88
3. Jameson DM, Sawyer WH 1995 Fluorescence anisotropy applied to biomolecular interactions. Methods Enzymol 246:283-300
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6. Lefstin JA, Thomas JR, Yamamoto KR 1994 Influence of a steroid receptor DNA-binding domain on transcriptional regulatory functions. Genes Dev 8: 2842-56
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10. Heyduk T, Lee JC 1990 Application of fluorescence energy transfer and polarization to monitor Escherichia coli cAMP receptor protein and lac promoter interaction. Proc Natl Acad Sci USA 87:1744-1748
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