Double labelling of subcellular structures with organelle-targeted GFP mutants in vivo

Current Biology

Volumn 6, Issue 2, 1996, Pages 183-188

  Rizzuto, Rosario ^a   Brini, Marisa ^a   De Giorgi, Francesca ^a   Rossi, Raffaela ^a   Heim, Roger ^b   Tsien, Roger Y ^b   Pozzan, Tullio ^a  

^a UNIVERSITY OF PADOVA   (Italy)

^b HOWARD HUGHES MEDICAL INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN; PHOTOPROTEIN; PRIMER DNA;

ARTICLE; CELL ORGANELLE; COLOR; GENETICS; HELA CELL; HUMAN; METABOLISM; MOLECULAR CLONING; MOLECULAR GENETICS; MUTATION; NUCLEOTIDE SEQUENCE;

BASE SEQUENCE; CLONING, MOLECULAR; COLOR; DNA PRIMERS; GREEN FLUORESCENT PROTEINS; HELA CELLS; HUMANS; LUMINESCENT PROTEINS; MOLECULAR SEQUENCE DATA; MUTATION; ORGANELLES; RECOMBINANT FUSION PROTEINS;

EID: 0030087711     PISSN: 09609822     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0960-9822(02)00451-7     Document Type: Article

References (16)

1. Chalfie M, Tu Y, Euskirchen G, Ward WW, Prasher DC: Green fluorescent protein as a marker for gene expression. Science 1994, 263:802-805.
2. Wang S, Hazelrigg T: Implications for bcd mRNA localization from spatial distribution of exu protein in Drosophila oogenesis. Nature 1994, 369:400-403.
3. Rizzuto R, Brini M, Pizzo P, Murgia M, Pozzan T: Chimeric green fluorescent protein: a new tool for visualizing subcellular organelles in living cells. Curr Biol 1995, 5:635-642.
4. Ikawa M, Kominami K, Yoshimura Y, Tanaka K, Nishimune Y, Okabe M: Green fluorescent protein as a marker in transgenic mice. Dev Growth Differ 1995, 37:455-459.
5. Amsterdam A, Lin S, Hopkins N: The Aequorea victoria green fluorescent protein can be used as a reporter in live zebrafish embryos. Dev Biol 1995, 171:123-129.
6. Yeh E, Gustafson K, Boulianne GL: Green fluorescent protein as a vital marker and reporter of gene expression in Drosophila. Proc Natl Acad Sci USA 1995, 92:7036-7040.
7. Heim R, Cubitt AB, Tsien RY: Improved green fluorescence. Nature 1995, 373:663-664.
8. Heim R, Prasher D, Tsien RY: Wavelength mutations and posttranslational autoxidation of green fluorescent protein. Proc Natl Acad Sci USA 1994, 91:12501-12504.
9. Picard D, Yamamoto KR: Two signals mediate hormone-dependent nuclear localization of the glucocorticoid receptor. EMBO J 1987, 6:3333-3340.
10. 2+ concentration measured with specifically targeted recombinant aequorin. EMBO J 1993, 12:4813-4819.
11. 2+ concentration in intact cells. Cell Calcium 1994, 16:259-268.
12. Cubitt, AB, Heim, R, Adams, SR, Boyd, AE, Gross, LA, Tsien, RY: Understanding, improving and using green fluorescent proteins. Trends Biochem Sci 1995, 20:448-455.
13. Heim, R, Tsien, RY: Engineering green fluorescent protein for improved brightness, longer wavelengths, and fluorescence resonance energy transfer. Curr Biol 1996, 6:178-182.
14. Rizzuto R, Nakase H, Darras B, Fabrizi GM, Mengel T, Walsh F, et al.: A gene specifying subunit VIII of human cytochrome c oxidase is localized to chromosome 11 and is expressed in both muscle and non- muscle tissues. J Biol Chem 1989, 264:10595-10600.
15. Field J, Nikawa J, Broeck D, MacDonald B, Rodgers L, Wilson IA, et al.: Purification of a RAS-responsive adenyl-cyclase complex from Saccaromyces cerevisiae by use of an epitope addition method. Mol Cell Biol 1988, 8:2159-2165.
16. 2+] in mitochondria of living cells. Methods Enzymol 1995, 260:417-428.