Fluorescence correlation spectrometry of the interaction kinetics of tetramethylrhodamin α-bungarotoxin with Torpedo californica acetylcholine receptor

Biophysical Chemistry

Volumn 58, Issue 1-2, 1996, Pages 3-12

  Rauer, Barbara ^a   Neumann, Eberhard ^a   Widengren, Jerker ^b   Rigler, Rudolf ^b  

^a BIELEFELD UNIVERSITY   (Germany)

^b KAROLINSKA INSTITUTE   (Sweden)

Author keywords

Acetylcholine receptor; Fluctuation kinetics; Fluorescence correlation spectroscopy; Bungarotoxin

Indexed keywords

ALPHA BUNGAROTOXIN; CHOLINERGIC RECEPTOR;

ANIMAL TISSUE; ARTICLE; BINDING KINETICS; CONTROLLED STUDY; DRUG RECEPTOR BINDING; FLUORESCENCE; NONHUMAN; PRIORITY JOURNAL; TECHNIQUE; TORPEDO CALIFORNICA;

EID: 0030024020     PISSN: 03014622     EISSN: None     Source Type: Journal    
DOI: 10.1016/0301-4622(95)00080-1     Document Type: Article

References (15)

1. A. Karlin, Exploration of the nicotinic acetylcholine receptor, Harvey Lectures, 85 (1991) 71-107.
2. H.W. Chang, E. Bock and E. Neumann, Long-lived metastable states and hysteresis in the binding of acetylcholine to Torpedo californica acetylcholine receptor, Biochemistry, 23 (1984) 4546-4556.
3. T. Schürholz, J. Kehne, A. Gieselmann and E. Neumann, Functional reconstitution of the nicotinic acetylcholine receptor by CHAPS dialysis depends on the concentration of salt, lipid and protein, Biochemistry, 31 (1992) 5067-5077.
4. R. Rigler, U. Mets, J. Widengren and P. Kask, Fluorescence correlation spectroscopy with high count rate and low background: analysis of translational diffusion, Eur. Biophys. J., 22 (1993) 169-175.
5. R. Rigler, J. Widengren and U. Mets, Interactions and kinetics of single molecules as observed by fluorescence correlation spectroscopy, in O.S. Wolfbeis (Editor), Fluorescence Spectroscopy, Springer-Verlag, Berlin, 1992, pp. 13-24.
6. J. Widengren, R. Rigler and U. Mets, Triplet-state monitoring by fluorescence correlation spectroscopy, J. Fluoresc., 4 (1994) 255-258.
7. T. Endo, M. Nakanishi, S. Furukawa, F.J. Joubert, N. Tamiya and K. Hayashi, Stopped-flow fluorescence studies on binding kinetics of neurotoxins with acetylcholine receptor, Biochemistry, 25 (1986) 395-404.
8. 125I]-α-bungarotoxin with acetylcholine receptor from Torpedo californica, Biochemistry, 18 (1979) 1875-1883.
9. R.J. Lukas, H. Morimoto, M.R. Hanley and E.L. Bennett, Radiolabeled α-bungarotoxin derivatives: kinetic interaction with nicotinic acetylcholine receptors, Biochemistry, 20 (1981) 7373-7378.
10. A. Maelicke, B.W. Fulpius, R.P. Klett and E. Reich, Acetylcholine receptor responses to drug binding, J. Biol. Chem., 252 (1977) 4811-4830.
11. G. Weiland, B. Georgia, V.T. Wee, C.F. Chignell and P. Taylor, Ligand interaction with cholinergic receptor-enriched membranes from Torpedo: influence of agonist exposure on receptor properties, Mol. Pharmacol., 12 (1976) 1091-1105.
12. S. Kang and A. Maelicke, Fluorescein isothiocyanate-labeled α-cobratoxin: Biochemical characterization and interaction with acetylcholine receptor from Electrophorus electricus, J. Biol. Chem., 256 (1980) 7326-7332.
13. B.M. Conti-Tronconi, F. Tang, S. Walgrave and W. Gallagher, Nonequivalence of α-bungarotoxin binding sites in the native nicotinic receptor molecule, Biochemistry, 29 (1990) 1046-1054.
14. M. Eigen and L. DeMaeyer, Relaxation methods, in S.L. Friess, E.S. Lewis and A. Weissberger (Editors), Techniques of Organic Chemistry, Vol. 8(2), Wiley, New York, 1963, pp. 895-1054.
15. E. Neumann and H.W. Chang, Dynamic properties of isolated acetylcholine receptor protein: kinetics of the binding of acetylcholine and Ca ions, Proc. Natl. Acad. Sci. USA, 73 (1976) 3994-3998.