A nickel chelate microtiter plate assay for six histidine-containing proteins

Analytical Biochemistry

Volumn 234, Issue 1, 1996, Pages 60-65

  Paborsky, Lisa R ^a   Dunn, Kyla E ^a   Gibbs, Craig S ^a   Dougherty, Joseph P ^a  

^a GILEAD SCIENCES   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACIDS; CHELATION; METAL IONS; NICKEL;

CARBOXYL TERMINUS; HIGH THROUGHPUT SCREENING; HISTIDINE RESIDUES; NITRILOTRIACETIC ACID; PROTEIN CONCENTRATIONS; PROTEIN PURIFICATION; PROTEIN-LIGAND INTERACTIONS; PROTEIN-PROTEIN INTERACTIONS;

RECOMBINANT PROTEINS;

HISTIDINE; IMIDAZOLE; LIGAND; LYSINE DERIVATIVE; MALEIC ANHYDRIDE; METAL ION; NICKEL; NITRILOTRIACETIC ACID; POLYSTYRENE; PROTEIN; RECOMBINANT PROTEIN; RESIN;

AMINO TERMINAL SEQUENCE; ARTICLE; ASSAY; BINDING COMPETITION; CARBOXY TERMINAL SEQUENCE; ENZYME LINKED IMMUNOSORBENT ASSAY; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN PROTEIN INTERACTION; PROTEIN PURIFICATION;

EID: 0030069360     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1006/abio.1996.0050     Document Type: Article

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