Ethanol induces expression of the glutamate transporters EAAT1 and EAAT2 in organotypic cortical slice cultures

Alcoholism: Clinical and Experimental Research

Volumn 28, Issue 11, 2004, Pages 1752-1757

  Zink, Mathias ^a   Schmitt, Andrea ^a   Vengeliene, Valentine ^a   Henn, Fritz A ^a   Spanagel, Rainer ^a  

^a CENTRAL INSTITUTE OF MENTAL HEALTH   (Germany)

Author keywords

Development; EAAT; Ethanol; Fetal Alcohol Syndrome; Glutamate

Indexed keywords

EXCITATORY AMINO ACID TRANSPORTER; GLUTAMATE TRANSPORTER; HYPOPHYSIS ADENYLATE CYCLASE ACTIVATING POLYPEPTIDE; NEUROPEPTIDE;

ANIMAL TISSUE; ARTICLE; ASTROCYTE; BRAIN DEVELOPMENT; BRAIN SLICE; COMPETITIVE INHIBITION; FETAL ALCOHOL SYNDROME; GENE EXPRESSION REGULATION; MEMBRANE BINDING; NERVE CELL CULTURE; NERVE FIBER GROWTH; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; RAT; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SYNAPTOGENESIS; TRANSCRIPTION REGULATION; WESTERN BLOTTING;

AMINO ACID TRANSPORT SYSTEM X-AG; ANIMALS; CEREBRAL CORTEX; ETHANOL; EXCITATORY AMINO ACID TRANSPORTER 1; EXCITATORY AMINO ACID TRANSPORTER 2; GENE EXPRESSION REGULATION; GLUTAMATE PLASMA MEMBRANE TRANSPORT PROTEINS; ORGAN CULTURE TECHNIQUES; RATS; RATS, WISTAR; SYMPORTERS;

ANIMALIA;

EID: 9244260589     PISSN: 01456008     EISSN: None     Source Type: Journal    
DOI: 10.1097/01.ALC.0000145810.12545.B3     Document Type: Article

References (34)

1. Behar TN, Scott CA, Greene CL, Wen X, Smith SV, Marie D, Liu Q-Y, Colton CA, Barker JL (1999) Glutamate acting at NMDA receptors stimulates embryonic cortical neuronal migration. J Neurosci 19:4449-4461.
2. Belmadani A, Neafsey EJ, Collins MA (2003) Human immunodeficiency virus type 1 gp120 and ethanol coexposure in rat organotypic brain slice cultures: curtailment of gp120-induced neurotoxicity and neurotoxic mediators by moderate but not high ethanol concentrations. J Neurovirol 9:45-54.
3. Burd L, Klug MG, Martsolf JT (2004) Increased sibling mortality in children with fetal alcohol syndrome. Addict Biol 9:179-186.
4. Butters NS, Reynolds JN, Brien JF (2003) Effects of chronic prenatal ethanol exposure on cGMP content and glutamate release in the hippocampus of the neonatal guinea pig. Neurotoxicol Teratol 25:59-68.
5. Danbolt NC (2001) Glutamate uptake. Progr Neurobiol 65:1-105.
6. Devaud LL (2001) Ethanol dependence has limited effects on GABA or glutamate transporters in rat brain. Alcohol Clin Exp Res 25:606-611.
7. Diao L, Dunwiddie TV (1996) Interactions between ethanol, endogenous adenosine and adenosine uptake in hippocampal brain slices. J Pharmacol Exp Ther 278:542-546.
8. Dobbing J, Sands J (1979) Comparative aspects of the brain growth spurt. Early Hum Dev 3:79-83.
9. Faingold C, Li Y, Evans MS (2000) Decreased GABA and increased glutamate receptor-mediated activity on inferior colliculus neurons in vitro are associated with susceptibility to ethanol withdrawal seizures. Brain Res 868:287-295.
10. Figiel M, Engele J (2000) Pituitary adenylate cyclase-activating polypeptide (PACAP), a neuron-derived peptide regulating glial glutamate transport and metabolism. J Neurosci 20:3596-3605.
11. Furuta A, Rothstein JD, Martin LJ (1997) Glutamate transporter protein subtypes are expressed differentially during rat CNS development. J Neurosci 17:8363-8375.
12. Gibson DA, Harris BR, Prendergast MA, Hart SR, Blanchard JA II, Holley RC, Pedigo NW, Littleton JM (2003) Polyamines contribute to ethanol withdrawal-induced neurotoxicity in rat hippocampal slice cultures through interactions with the NMDA receptor. Alcohol Clin Exp Res 27:1099-1106.
13. Harris BR, Gibson DA, Prendergast MA, Blanchard JA, Holley RC, Hart SR, et al. (2003) The neurotoxicity induced by ethanol withdrawal in mature organotypic hippocampal slices might involve cross-talk between metabotropic glutamate type 5 receptors and N-methyl-D-aspartate receptors. Alcohol Clin Exp Res 27:1724-1735.
14. Haydar TF, Bambrick LL, Krueger BK, Rakic P (1999) Organotypic slice cultures for analysis of proliferation, cell death, and migration in the embryonic neocortex. Brain Res Brain Res Protoc 4:425-437.
15. Hundt W, Danysz W, Holler SM, Spanagel R (1998) Ethanol and N-methyl-D-aspartate-receptor complex interactions: a detailed drug discrimination study in the rat. Psychopharmacology 135:44-51.
16. Ikonomidou C, Bittigau P, Ishimaru MJ, Wozniak DF, Koch C, Genz K, et al. (2000) Ethanol-induced apoptotic neurodegeneration and fetal alcohol syndrome. Science 287:1056-1060.
17. Ikonomidou C, Bosch F, Miksa M, Bittigau P, Vöckler J, Dikranian K, et al. (1999) Blockade of NMDA receptors and apoptotic neurodegeneration in the developing brain. Science 283:70-74.
18. Lovinger DM, White G, Weight FF (1989) Ethanol inhibits NMDA-activated ion current in hippocampal neurons. Science 243:1721-1724.
19. Molnar Z, Blakemore C (1999) Development of signals influencing the growth and termination of thalamocortical axons in organotypic culture. Exp Neurol 156:363-393.
20. Olney JW (2004) Fetal alcohol syndrome at the cellular level. Addict Biol 9:137-149.
21. Olney JW, Wozniak DF, Farber NB, Jevtovic-Todorovic V, Bittigau P, Ikonomidou C (2002) The enigma of fetal alcohol neurotoxicity. Ann Med 34:109-119.
22. Olney JW, Wozniak DF, Jevtovic-Todorovic V, Ikonomidou C (2001) Glutamate signaling and the fetal alcohol syndrome. Ment Retard Dev Disabil Res Rev 7:267-275.
23. Othman T, Sinclair CJ, Haughey N, Geiger JD, Parkinson FE (2002) Ethanol alters glutamate but not adenosine uptake in rat astrocytes: evidence for protein kinase C involvement. Neurochem Res 27:289-296.
24. Perego C, Vanoni C, Bossi M, Massari S, Basudev H, Longhi R, Pietrini G, Pietrini G (2000) The GLT-1 and GLAST glutamate transporters are expressed on morphologically distinct astrocytes and regulated by neuronal activity in primary hippocampal cocultures. J Neurochem 75:1076-1084.
25. Schreiber R, Freund WD (2000) Glutamate transport is downregulated in the cerebral cortex of alcohol-preferring rats. Med Sci Monit 6:649-652.
26. Smith TL (1997) Regulation of glutamate uptake in astrocytes continuously exposed to ethanol. Life Sci 61:2499-2505.
27. Smith TL, Navratilova E (1999) Increased calcium/calmodulin protein kinase activity in astrocytes chronically exposed to ethanol: influences on glutamate transport. Neurosci Lett 269:145-148.
28. +-dependent high affinity uptake of glutamate in astrocytes chronically exposed to ethanol. Neurosci Lett 218:142-144.
29. Sutherland ML, Delaney TA, Noebels JL (1996) Glutamate transporter mRNA expression in proliferative zones of the developing and adult murine CNS. J Neurosci 16:2191-2207.
30. Szot P, White SS, Veith RC, Rasmussen DD (1999) Reduced gene expression for dopamine biosynthesis and transport in midbrain neurons of adult male rats exposed prenatally to ethanol. Alcohol Clin Exp Res 23:1643-1649.
31. Thomas MP, Monaghan DT, Morrisett RA (1998) Evidence for a causative role of N-methyl-D-aspartate receptors in an in vitro model of alcohol withdrawal hyperexcitability. J Pharmacol Exp Ther 287:87-97.
32. Thomas MP, Morrisett RA (2000) Dynamics of NMDAR-mediated neurotoxicity during chronic ethanol exposure and withdrawal. Neuropharmacology 39:218-226.
33. Torp R, Danbolt NC, Babaie E, Bjoras M, Seeberg E, Storm-Mathisen J, Ottersen OP (1994) Differential expression of two glial glutamate transporters in the rat brain: an in situ hybridization study. Eur J Neurosci 6:936-942.
34. Zafar H, Shelat SG, Redei E, Tejani-Butt S (2000) Fetal alcohol exposure alters serotonin transporter sites in rat brain. Brain Res 856:184-192.