Organotypic slice cultures for analysis of proliferation, cell death, and migration in the embryonic neocortex

Brain Research Protocols

Volumn 4, Issue 3, 1999, Pages 425-437

  Haydar, Tarik F ^a   Bambrick, Linda L ^b   Krueger, Bruce K ^b   Rakic, Pasko ^a  

^a YALE UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b UNIVERSITY OF MARYLAND SCHOOL OF MEDICINE   (United States)

Author keywords

Apoptosis; Migration; Neocortex; Organotypic; Proliferation

Indexed keywords

ANIMAL MODEL; ANIMAL TISSUE; APOPTOSIS; ARTICLE; CELL CULTURE; CELL CYCLE; CELL DEATH; CELL MIGRATION; CELL PROLIFERATION; CONTROLLED STUDY; EMBRYO; IMAGE ANALYSIS; IMMUNOCYTOCHEMISTRY; IN VITRO STUDY; INTRATHECAL DRUG ADMINISTRATION; MOUSE; NEOCORTEX; NERVOUS SYSTEM DEVELOPMENT; NONHUMAN; PRIORITY JOURNAL; TECHNIQUE;

ANIMALS; APOPTOSIS; BROMODEOXYURIDINE; CELL DIVISION; CELL MOVEMENT; CELL SURVIVAL; FEMALE; FETUS; FLUORESCENT ANTIBODY TECHNIQUE; GLIAL FIBRILLARY ACIDIC PROTEIN; IN SITU NICK-END LABELING; MICE; MICE, INBRED ICR; MICROSCOPY, CONFOCAL; MICROTUBULE-ASSOCIATED PROTEINS; NEOCORTEX; NEUROGLIA; NEURONS; ORGAN CULTURE TECHNIQUES; PREGNANCY;

EID: 0344374736     PISSN: 1385299X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1385-299X(99)00033-1     Document Type: Article

References (19)

1. Rakic P. A small step for the cell, a giant leap for mankind: a hypothesis of neocortical expansion during evolution. Trends Neurosci. 18:1995;383-388.
2. Caviness V.S. Jr., Takahashi T., Nowakowski R.S. Numbers, time and neocortical neuronogenesis: a general developmental and evolutionary model. Trends Neurosci. 18:1995;379-383.
3. LoTurco J.J., Owens D.F., Heath M.J., Davis M.B., Kriegstein A.R. Gaba and glutamate depolarize cortical progenitor cells and inhibit DNA synthesis. Neuron. 15:1995;1287-1298.
4. Kuida K., Haydar T.F., Kuan C-Y., Gu Y., Taya C., Karasuyama H., Su M.S., Rakic P., Flavell R.A. Reduced apoptosis and cytochrome c-mediated caspase activation in mice lacking caspase 9. Cell. 94:1998;325-337.
5. Rakic P., Cameron R.S., Komuro H. Recognition, adhesion, transmembrane signaling and cell motility in guided neuronal migration. Curr. Opin. Neurobiol. 4:1994;63-69.
6. Bittman K., Owens D.F., Kriegstein A.R., LoTurco J.J. Cell coupling and uncoupling in the ventricular zone of developing neocortex. J. Neurosci. 17:1997;7037-7044.
7. Rakic P., Sidman R.L. Supravital DNA synthesis in the developing human and mouse brain. J. Neuropath. Exp. Neurol. 27:1968;246-276.
8. Martin J.S., Reutelingsperger C.P., McGahon A.J., Rader J.A., van Schie R.C., LaFace D.M., Green D.R. Early redistribution of plasma membrane phosphatidylserine is a general feature of apoptosis regardless of initiating stimulus: Inhibition by overexpression by Bcl-2 and Abl. J. Exp. Med. 182:1995;1545-1556.
9. Gavrieli Y., Sherman Y., Ben-Sasson S.A. Identification of programmed cell death in situ via specific labeling of nuclear DNA fragmentation. J. Cell Biol. 119:1992;493-501.
10. Spreafico R., Frassoni C., Arcelli P., Selvaggio M., De Biasi S. In situ labeling of apoptotic cell death in the cerebral cortex and thalamus of rats during development. J. Comp. Neurol. 363:1995;281-295.
11. Sauer M.E., Chittenden A.C. Deoxyribonucleic acid content of cell nuclei in the neural tube of the chick embryo: evidence for intermitotic migration of nuclei. Exp. Cell Res. 16:1959;1-6.
12. Takahashi T., Nowakowski R.S., Caviness V.S. Jr. Cell cycle parameters and patterns of nuclear movement in the neocortical proliferative zone of the fetal mouse. J. Neurosci. 13:1993;820-833.
13. Takahashi T., Nowakowski R.S., Caviness V.S. Jr. The cell cycle of the pseudostratified ventricular epithelium of the embryonic murine cerebral wall. J. Neurosci. 15:1995;6046-6057.
14. Cameron H.A., Hazel T.G., McKay R.D.G. Regulation of neurogenesis by growth factors and neurotransmitters. J. Neurobiol. 36:1998;287-306.
15. Gahwiler B.H., Capogna M., Debanne D., McKinney R.A., Thompson S.M. Organotypic slice cultures: a technique has come of age. Trends Neurosci. 20:1997;471-477.
16. Stoppini L., Buchs P-A., Muller D. A simple method for organotypic cultures of nervous tissue. J. Neurosci. Methods. 37:1991;173-182.
17. Takahashi T., Bhide P.G., Goto T., Miyama S., S Caviness V. Jr. Proliferative behavior of the murine cerebral wall in tissue culture: cell cycle kinetics and checkpoints. Exp. Neurol. 156:1999;407-417.
18. Thomaidou D., Mione M.C., Cavanaugh J.F.R., Parnavelas J.G. Apoptosis and its relation to the cell cycle in the developing cerebral cortex. J. Neurosci. 17:1997;1075-1085.
19. T. Haydar, Abnormal cortical histogenesis of the Trisomy 16 mouse, Thesis (1997) University of Maryland School of Medicine.