Cloning of the Pneumocystis jirovecii trifunctional FAS gene and complementation of its DHPS activity in Escherichia coli

Fungal Genetics and Biology

Volumn 41, Issue 12, 2004, Pages 1053-1062

  Iliades, Peter ^a   Walker, Daniel J ^b   Castelli, Laura ^a   Satchell, Jacqueline ^c   Meshnick, Steven R ^b   MacReadie, Ian G ^a  

^a CSIRO   (Australia)

^b UNIVERSITY OF NORTH CAROLINA   (United States)

^c WALTER AND ELIZA HALL INSTITUTE OF MEDICAL RESEARCH   (Australia)

Author keywords

AIDS; dapsone; Dihydroneopterin aldolase; Dihydropteroate synthase; FAS gene; Folic acid synthesis; Hydroxymethyldihydropterin pyrophosphokinase; PCP; Pneumocystis carinii; Pneumocystis jirovecii; Pneumonia; Sulfa drugs; Sulfamethoxazole

Indexed keywords

DIHYDRONEOPTERIN ALDOLASE; DIHYDROPTEROATE SYNTHASE; FAS ANTIGEN; GLUTATHIONE TRANSFERASE; HYDROXYMETHYLDIHYDROPTERIN PYROPHOSPHOKINASE; NEOPTERIN; PHOSPHOTRANSFERASE; SULFANILAMIDE; UNCLASSIFIED DRUG;

AMINO TERMINAL SEQUENCE; ARTICLE; BACTERIAL STRAIN; CONTROLLED STUDY; ESCHERICHIA COLI; GENE EXPRESSION; GENE FUNCTION; GENE MUTATION; GENE SEQUENCE; GENETIC COMPLEMENTATION; GENETIC CONSERVATION; INTRON; LUNG LAVAGE; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PATIENT; PNEUMOCYSTIS; PNEUMOCYSTIS CARINII; PNEUMOCYSTIS JIROVECI; PNEUMOCYSTIS PNEUMONIA; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL;

ALDEHYDE-LYASES; AMINO ACID SEQUENCE; ANTI-INFECTIVE AGENTS; BRONCHOALVEOLAR LAVAGE FLUID; CONSERVED SEQUENCE; DIHYDROPTEROATE SYNTHASE; DIPHOSPHOTRANSFERASES; DNA, FUNGAL; DRUG RESISTANCE, FUNGAL; ESCHERICHIA COLI; FUNGAL PROTEINS; GENES, FUNGAL; GENETIC COMPLEMENTATION TEST; HUMANS; INTRONS; MOLECULAR SEQUENCE DATA; PNEUMOCYSTIS JIROVECI; PNEUMONIA, PNEUMOCYSTIS; RECOMBINANT PROTEINS; SEQUENCE ANALYSIS, DNA; SEQUENCE HOMOLOGY, AMINO ACID; SULFANILAMIDES;

ESCHERICHIA COLI; MACACA; PNEUMOCYSTIS CARINII; PNEUMOCYSTIS CARINII F. SP. MACACA; PNEUMOCYSTIS JIROVECII;

EID: 8644250743     PISSN: 10871845     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.fgb.2004.08.006     Document Type: Article

References (42)

1. A. Achari, D.O. Somers, J.N. Champness, P.K. Bryant, J. Rosemond, and D.K. Stammers Crystal structure of the anti-bacterial sulfonamide drug target dihydropteroate synthase Nat. Struct. Biol. 4 1997 490 497
2. N.A. Ansari, A.H. Kombe, T.A. Kenyon, L. Mazhani, N. Binkin, J.W. Tappero, T. Gebrekristos, S. Nyirenda, and S.B. Lucas Pathology and causes of death in a series of human immunodeficiency virus-positive and -negative pediatric referral hospital admissions in Botswana Pediatr. Infect. Dis. J. 22 2003 43 47
3. W. Armstrong, S. Meshnick, and P. Kazanjian Pneumocystis carinii mutations associated with sulfa and sulfone prophylaxis failures in immunocompromised patients Microbes Infect. 2 2000 61 67
4. A.M. Baca, R. Sirawaraporn, S. Turley, W. Sirawaraporn, and W.G. Hol Crystal structure of Mycobacterium tuberculosis 7,8-dihydropteroate synthase in complex with pterin monophosphate: new insight into the enzymatic mechanism and sulfa-drug action J. Mol. Biol. 302 2000 1193 1212
5. L.A. Castelli, N.P. Nguyen, and I.G. Macreadie Sulfa drug screening in yeast: fifteen sulfa drugs compete with p-aminobenzoate in Saccharomyces cerevisiae FEMS Microbiol. Lett. 199 2001 181 184
6. C. Demanche, J. Guillot, M. Berthelemy, T. Petitt, P. Roux, and A.E. Wakefield Absence of mutations associated with sulfa resistance in Pneumocystis carinii dihydropteroate synthase gene from non-human primates Med. Mycol. 40 2002 315 318
7. C. Fermer, and G. Swedberg Adaptation to sulfonamide resistance in Neisseria meningitidis may have required compensatory changes to retain enzyme function: kinetic analysis of dihydropteroate synthases from N. meningitidis expressed in a knockout mutant of Escherichia coli J. Bacteriol. 179 1997 831 837
8. U. Guldener, G.J. Koehler, C. Haussmann, A. Bacher, J. Kricke, D. Becher, and J.H. Hegemann Characterization of the Saccharomyces cerevisiae Foll Protein: Starvation for C1 Carrier Induces Pseudohyphal Growth Mol. Biol. Cell. 15 2004 3811 3828
9. D.J. Hakes, and J.E. Dixon New vectors for high level expression of recombinant proteins in bacteria Anal. Biochem. 202 1992 293 298
10. I.C. Hampele, A. D'Arcy, G.E. Dale, D. Kostrewa, J. Nielsen, C. Oefner, M.G. Page, H.J. Schonfeld, D. Stuber, and R.L. Then Structure and function of the dihydropteroate synthase from Staphylococcus aureus J. Mol. Biol. 268 1997 21 30
11. J. Helweg-Larsen, T.L. Benfield, J. Eugen-Olsen, J.D. Lundgren, and B. Lundgren Effects of mutations in Pneumocystis carinii dihydropteroate synthase gene on outcome of AIDS-associated P. carinii pneumonia Lancet 354 1999 1347 1351
12. Y.L. Hong, P.A. Hossler, D.H. Calhoun, and S.R. Meshnick Inhibition of recombinant Pneumocystis carinii dihydropteroate synthetase by sulfa drugs Antimicrob. Agents Chemother. 39 1995 1756 1763
13. P. Iliades, J. Berglez, S. Meshnick, and I. Macreadie Promoter strength of folic acid synthesis genes affects sulfa drug resistance in Saccharomyces cerevisiae Microb. Drug Resist. 9 2003 249 255
14. P. Iliades, S.R. Meshnick, and I.G. Macreadie Dihydropteroate synthase mutations in Pneumocystis jiroveci can affect sulfamethoxazole resistance in a Saccharomyces cerevisiae Model Antimicrob. Agents Chemother. 48 2004 2617 2623
15. Iliades, P., Meshnick, S.R., Macreadie, I.G., 2004. Analysis of Pneumocystis jiroveci DHPS alleles implicated in sulfamethoxazole resistance using an Escherichia coli model system. Microb. Drug Resist. (in press)
16. J.E. Kaplan, D.L. Hanson, T.R. Navin, and J.L. Jones Risk factors for primary Pneumocystis carinii pneumonia in human immunodeficiency virus-infected adolescents and adults in the United States: reassessment of indications for chemoprophylaxis J. Infect. Dis. 178 1998 1126 1132
17. P. Kazanjian, W. Armstrong, P.A. Hossler, W. Burman, J. Richardson, C.H. Lee, L. Crane, J. Katz, and S.R. Meshnick Pneumocystis carinii mutations are associated with duration of sulfa or sulfone prophylaxis exposure in AIDS patients J. Infect. Dis. 182 2000 551 557
18. P. Kazanjian, A.B. Locke, P.A. Hossler, B.R. Lane, M.S. Bartlett, J.W. Smith, M. Cannon, and S.R. Meshnick Pneumocystis carinii mutations associated with sulfa and sulfone prophylaxis failures in AIDS patients AIDS 12 1998 873 878
19. J.A. Kovacs, V.J. Gill, S. Meshnick, and H. Masur New insights into transmission, diagnosis, and drug treatment of Pneumocystis carinii pneumonia J. Am. Med. Assoc. 286 2001 2450 2460
20. B.R. Lane, J.C. Ast, P.A. Hossler, D.P. Mindell, M.S. Bartlett, J.W. Smith, and S.R. Meshnick Dihydropteroate synthase polymorphisms in Pneumocystis carinii J. Infect. Dis. 175 1997 482 485
21. L. Ma, L. Borio, H. Masur, and J.A. Kovacs Pneumocystis carinii dihydropteroate synthase but not dihydrofolate reductase gene mutations correlate with prior trimethoprim- sulfamethoxazole or dapsone use J. Infect. Dis. 180 1999 1969 1978
22. L. Ma, J.A. Kovacs, A. Cargnel, A. Valerio, G. Fantoni, and C. Atzori Mutations in the dihydropteroate synthase gene of human-derived Pneumocystis carinii isolates from Italy are infrequent but correlate with prior sulfa prophylaxis J. Infect. Dis. 185 2002 1530 1532
23. T.K. Marras, K. Sanders, J.H. Lipton, H.A. Messner, J. Conly, and C.K. Chan Aerosolized pentamidine prophylaxis for Pneumocystis carinii pneumonia after allogeneic marrow transplantation Transpl. Infect. Dis. 4 2002 66 74
24. Q. Mei, S. Gurunathan, H. Masur, and J.A. Kovacs Failure of co-trimoxazole in Pneumocystis carinii infection and mutations in dihydropteroate synthase gene Lancet 351 1998 1631 1632
25. I. Meneau, D. Sanglard, J. Bille, and P.M. Hauser Site-directed mutagenesis of the Saccharomyces cerevisiae dihydropteroate synthase FOL1 gene to study Pneumocystis jirovecii mutations in the orthologue gene FAS J. Eukaryot. Microbiol. 50 Suppl. 2003 652 653
26. I. Meneau, D. Sanglard, J. Bille, and P.M. Hauser Pneumocystis jiroveci dihydropteroate synthase polymorphisms confer resistance to sulfadoxine and sulfanilamide in Saccharomyces cerevisiae Antimicrob. Agents Chemother. 48 2004 2610 2616
27. A. Nahimana, M. Rabodonirina, J. Helweg-Larsen, I. Meneau, P. Francioli, J. Bille, and P.M. Hauser Sulfa resistance and dihydropteroate synthase mutants in recurrent Pneumocystis carinii pneumonia Emerg. Infect. Dis. 9 2003 864 867
28. A. Nahimana, M. Rabodonirina, G. Zanetti, I. Meneau, P. Francioli, J. Bille, and P.M. Hauser Association between a specific Pneumocystis jiroveci dihydropteroate synthase mutation and failure of pyrimethamine/sulfadoxine prophylaxis in human immunodeficiency virus-positive and -negative patients J. Infect. Dis. 188 2003 1017 1023
29. T.R. Navin, C.B. Beard, L. Huang, C. del Rio, S. Lee, N.J. Pieniazek, J.L. Carter, T. Le, A. Hightower, and D. Rimland Effect of mutations in Pneumocystis carinii dihydropteroate synthase gene on outcome of P. carinii pneumonia in patients with HIV-1: a prospective study Lancet 358 2001 545 549
30. O. Patel, J. Satchell, J. Baell, R. Fernley, P. Coloe, and I. Macreadie Inhibition studies of sulfonamide-containing folate analogs in yeast Microb. Drug Resist. 9 2003 139 146
31. F.J. Robberts, L.J. Chalkley, and L.D. Liebowitz Pneumocystis pneumonia SADJ 57 2002 451 453
32. P.G. Sammes Sulfonamides and Sulfones. The Rational Design, Mechanistic Study and Therapeutic Application of Chemical Compounds: Enzymes and Other Molecules 1990 Pergamon Press Oxford
33. R.J. Simonds, W.T. Hughes, J. Feinberg, and T.R. Navin Preventing Pneumocystis carinii pneumonia in persons infected with human immunodeficiency virus Clin. Infect. Dis. 21 Suppl. 1 1995 S44 S48
34. J.R. Stringer, C.B. Beard, R.F. Miller, and A.E. Wakefield A new name (Pneumocystis jiroveci) for Pneumocystis from humans Emerg. Infect. Dis. 8 2002 891 896
35. T. Takahashi, N. Hosoya, T. Endo, T. Nakamura, H. Sakashita, K. Kimura, K. Ohnishi, Y. Nakamura, and A. Iwamoto Relationship between mutations in dihydropteroate synthase of Pneumocystis carinii f. sp. Hominis isolates in japan and resistance to sulfonamide therapy J. Clin. Microbiol. 38 2000 3161 3164
36. M.C. Thomas, S.P. Ballantine, S.S. Bethell, S. Bains, P. Kellam, and C.J. Delves Single amino acid substitutions disrupt tetramer formation in the dihydroneopterin aldolase enzyme of Pneumocystis carinii Biochemistry 37 1998 11629 11636
37. T. Triglia, J.G. Menting, C. Wilson, and A.F. Cowman Mutations in dihydropteroate synthase are responsible for sulfone and sulfonamide resistance in Plasmodium falciparum Proc. Natl. Acad. Sci. USA 94 25 1997 13944 13949
38. E. Visconti, E. Ortona, P. Mencarini, P. Margutti, S. Marinaci, M. Zolfo, A. Siracusano, and E. Tamburrini Mutations in dihydropteroate synthase gene of Pneumocystis carinii in HIV patients with Pneumocystis carinii pneumonia Int. J. Antimicrob. Agents 18 2001 547 551
39. F. Volpe, S.P. Ballantine, and C.J. Delves The multifunctional folic acid synthesis fas gene of Pneumocystis carinii encodes dihydroneopterin aldolase, hydroxymethyldihydropterin pyrophosphokinase and dihydropteroate synthase Eur. J. Biochem. 216 1993 449 458
40. F. Volpe, M. Dyer, J.G. Scaife, G. Darby, D.K. Stammers, and C.J. Delves The multifunctional folic acid synthesis fas gene of Pneumocystis carinii appears to encode dihydropteroate synthase and hydroxymethyldihydropterin pyrophosphokinase Gene 112 1992 213 218
41. T. Zhu, and D.D. Ho Was HIV present in 1959? Nature 374 1995 503 504
42. A. Zingale, P. Carrera, A. Lazzarin, and P. Scarpellini Detection of Pneumocystis carinii and characterization of mutations associated with sulfa resistance in bronchoalveolar lavage samples from human immunodeficiency virus-infected subjects J. Clin. Microbiol. 41 2003 2709 2712