Identification of Absidia orchidis steroid 11β-hydroxylation system and its application in engineering Saccharomyces cerevisiae for one-step biotransformation to produce hydrocortisone

Metabolic Engineering

Volumn 57, Issue , 2020, Pages 31-42

  Chen, Jing ^a,b,c   Fan, Feiyu ^a,b   Qu, Ge ^a   Tang, Jinlei ^a,b   Xi, Yongyan ^a,b,c   Bi, Changhao ^a,b   Sun, Zhoutong ^a   Zhang, Xueli ^a,b  

^a Chinese Academy of Sciences   (China)

^b INSTITUTE OF GEOLOGY AND GEOPHYSICS   (China)

^c UNIVERSITY OF CHINESE ACADEMY OF SCIENCES   (China)

Author keywords

11 hydroxylation; Absidia orchidis; Hydrocortisone; Saccharomyces cerevisiae; Steroid; Transporter

Indexed keywords

BIOCONVERSION; CAPILLARY FLOW; GENES; HYDROXYLATION; PRODUCTIVITY; STEREOCHEMISTRY; YEAST;

ABSIDIA ORCHIDIS; ANTI-INFLAMMATORY DRUGS; BIOTRANSFORMATION SYSTEM; CYTOCHROME P450 ENZYMES; CYTOCHROME P450 REDUCTASE; PROTEIN ENGINEERING; STEROID; TRANSPORTER;

CORTISOL;

CYTOCHROME B5; CYTOCHROME P450; CYTOCHROME P450 5311 B2; CYTOCHROME P450 REDUCTASE; HYDROCORTISONE; STEROID 11BETA MONOOXYGENASE; UNCLASSIFIED DRUG; CORTODOXONE; FUNGAL PROTEIN;

ABSIDIA; ABSIDIA ORCHIDIS; ARTICLE; BIOTECHNOLOGICAL PRODUCTION; BIOTRANSFORMATION; CONTROLLED STUDY; CURVULARIA LUNATA; DRUG SYNTHESIS; ELIMINATION REACTION; GENE EXPRESSION; GENE OVEREXPRESSION; HYDROXYLATION; NONHUMAN; PRIORITY JOURNAL; PROTEIN ENGINEERING; SACCHAROMYCES CEREVISIAE; STEREOISOMERISM; TRANSCRIPTOMICS; BIOSYNTHESIS; ENZYMOLOGY; GENETICS; METABOLIC ENGINEERING; METABOLISM;

ABSIDIA; BIOTRANSFORMATION; CORTODOXONE; CYTOCHROME P-450 ENZYME SYSTEM; FUNGAL PROTEINS; HYDROCORTISONE; HYDROXYLATION; METABOLIC ENGINEERING; SACCHAROMYCES CEREVISIAE;

EID: 85074154351     PISSN: 10967176     EISSN: 10967184     Source Type: Journal    
DOI: 10.1016/j.ymben.2019.10.006     Document Type: Article

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