A recombinant CYP11B1 dependent Escherichia coli biocatalyst for selective cortisol production and optimization towards a preparative scale

Microbial Cell Factories

Volumn 14, Issue 1, 2015, Pages

  Schiffer, Lina ^a   Anderko, Simone ^a   Hobler, Anna ^a   Hannemann, Frank ^a   Kagawa, Norio ^a   Bernhardt, Rita ^a  

^a SAARLAND UNIVERSITY   (Germany)

Author keywords

Cortisol; E. coli; Human CYP11B1; Steroid biotransformation; Whole cell biocatalysis

Indexed keywords

ARGININE; CORTODOXONE; CYTOCHROME P450 11B1; CYTOCHROME P450 ISOENZYME; ENZYME VARIANT; GLYCINE; HYDROCORTISONE; UNCLASSIFIED DRUG; ADRENODOXIN; RECOMBINANT PROTEIN; STEROID 11BETA MONOOXYGENASE;

AMINO ACID SUBSTITUTION; ARTICLE; BACTERIAL CELL; BACTERIUM CULTURE; BACTERIUM TRANSFORMATION; BIOCATALYSIS; BIOCATALYST; BIOTRANSFORMATION; CONTROLLED STUDY; DRUG INDUSTRY; ELECTRON TRANSPORT; ESCHERICHIA COLI; GENE DOSAGE; GENETIC RECOMBINATION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HORMONE SYNTHESIS; HYDROCORTISONE RELEASE; HYDROXYLATION; MOLECULAR LIBRARY; NONHUMAN; PROCESS OPTIMIZATION; PRODUCTIVITY; ROBOTICS; SITE DIRECTED MUTAGENESIS; WESTERN BLOTTING; BIOSYNTHESIS; CHEMISTRY; GENETICS; HUMAN; METABOLISM; PROTEIN TERTIARY STRUCTURE;

ESCHERICHIA COLI;

ADRENODOXIN; BIOCATALYSIS; ESCHERICHIA COLI; HUMANS; HYDROCORTISONE; MUTAGENESIS, SITE-DIRECTED; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT PROTEINS; STEROID 11-BETA-HYDROXYLASE;

EID: 84924238487     PISSN: None     EISSN: 14752859     Source Type: Journal    
DOI: 10.1186/s12934-015-0209-5     Document Type: Article

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