Rediverting carbon flux in Clostridium ljungdahlii using CRISPR interference (CRISPRi)

Metabolic Engineering

Volumn 48, Issue , 2018, Pages 243-253

  Woolston, Benjamin M ^a   Emerson, David F ^a   Currie, Devin H ^a   Stephanopoulos, Gregory ^a  

^a Massachusetts Institute of Technology   (United States)

Author keywords

3HB; Acetogen; Autotrophic metabolic engineering; CO2 fixation; CRISPR interference; Syngas

Indexed keywords

CARBON; CARBON DIOXIDE; CLOSTRIDIUM; ENZYME ACTIVITY; GENES; METABOLISM; SYNTHESIS GAS; WOOD CHEMICALS; WOOD FUELS;

3-HYDROXYBUTYRATE; ACETOGEN; CLOSTRIDIUM LJUNGDAHLII; CO2 FIXATION; HETEROTROPHIC GROWTH; PHOSPHOTRANSACETYLASE; SYN-GAS; TRANSCRIPTIONAL REPRESSION;

METABOLIC ENGINEERING;

3 HYDROXYBUTYRIC ACID; CARBON DIOXIDE; PHOSPHATE ACETYLTRANSFERASE;

ARTICLE; BIOSYNTHESIS; BIOTRANSFORMATION; CELL GROWTH; CELL LYSATE; CLOSTRIDIUM; CLOSTRIDIUM LJUNGDAHLII; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEAT; CORYNEBACTERIUM GLUTAMICUM; CRISPR INTERFERENCE; CRISPR-CAS9 SYSTEM; CYTOLYSIS; DOWN REGULATION; ENZYME ACTIVITY; ENZYME ASSAY; ESCHERICHIA COLI; GENE TARGETING; METABOLIC ENGINEERING; MOLECULAR CLONING; NONHUMAN; OPERON; PRIORITY JOURNAL; PROTEIN DENATURATION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA ISOLATION; VECTOR CONTROL;

EID: 85049079035     PISSN: 10967176     EISSN: 10967184     Source Type: Journal    
DOI: 10.1016/j.ymben.2018.06.006     Document Type: Article

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