CRISPR/Cas9-Based Efficient Genome Editing in Clostridium ljungdahlii, an Autotrophic Gas-Fermenting Bacterium

ACS Synthetic Biology

Volumn 5, Issue 12, 2016, Pages 1355-1361

  Huang, He ^a   Chai, Changsheng ^a   Li, Ning ^a   Rowe, Pete ^b   Minton, Nigel P ^b   Yang, Sheng ^a,c   Jiang, Weihong ^a,c   Gu, Yang ^a,d  

^a INSTITUTE OF PLANT PHYSIOLOGY AND ECOLOGY   (China)

^b UNIVERSITY OF NOTTINGHAM   (United Kingdom)

^c Jiangsu National Synergetic Innovation Center for Advanced Materials   (China)

^d Shanghai Technology Innovation Center   (China)

Author keywords

autotrophic bacterium; Clostridium ljungdahlii; CRISPR Cas9; gas fermentation; rapid genome editing; strong promoters

Indexed keywords

ALCOHOL; BETA GALACTOSIDASE; GENOMIC DNA; GUIDE RNA; BACTERIAL PROTEIN;

ADHE1 GENE; ALLELE; ANTIBIOTIC RESISTANCE; ARTICLE; BACTERIAL GENE; BACTERIAL GENOME; CLOSTRIDIUM; CLOSTRIDIUM ACETOBUTYLICUM; CLOSTRIDIUM LJUNGDAHLII; CONTROLLED STUDY; CRISPR CAS SYSTEM; CRISPR CAS9 SYSTEM; CTF GENE; ESCHERICHIA COLI; FERMENTATION; GAS; GENE DELETION; GENE EXPRESSION; NONHUMAN; PRIORITY JOURNAL; PROMOTER REGION; PTA GENE; PYRE GENE; STREPTOCOCCUS PYOGENES; SYNTHESIS; GENE EDITING; GENETICS; METABOLISM; NUCLEOTIDE SEQUENCE; PLASMID;

BACTERIAL PROTEINS; BASE SEQUENCE; CLOSTRIDIUM; CRISPR-CAS SYSTEMS; FERMENTATION; GENE DELETION; GENE EDITING; PLASMIDS; PROMOTER REGIONS, GENETIC; RNA, GUIDE;

EID: 85006485809     PISSN: None     EISSN: 21615063     Source Type: Journal    
DOI: 10.1021/acssynbio.6b00044     Document Type: Article

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