Gene transcription repression in Clostridium beijerinckii using CRISPR-dCas9

Biotechnology and Bioengineering

Volumn 113, Issue 12, 2016, Pages 2739-2743

  Wang, Yi ^a   Zhang, Zhong Tian ^a   Seo, Seung Oh ^a   Lynn, Patrick ^a   Lu, Ting ^a,b   Jin, Yong Su ^a   Blaschek, Hans P ^a  

^a UNIVERSITY OF ILLINOIS AT URBANA CHAMPAIGN   (United States)

^b University of Illinois at Urbana Champaign   (United States)

Author keywords

amylase activity; CRISPR dCas9; CRISPRi; gene transcription repression; genome engineering; synthetic biology

Indexed keywords

AMYLASES; CLOSTRIDIUM; FERMENTATION; GENE EXPRESSION; GENES; NUCLEIC ACIDS; RNA;

AMYLASE ACTIVITY; CRISPR-DCAS9; CRISPRI; GENE TRANSCRIPTIONS; GENOME ENGINEERING; SYNTHETIC BIOLOGY;

TRANSCRIPTION;

ACETYL COENZYME A ACETYLTRANSFERASE; AGAR; AMYLASE; GUIDE RNA; STARCH; CRISPR ASSOCIATED PROTEIN;

ARTICLE; BACTERIAL GENE; BACTERIAL GENOME; BACTERIAL GROWTH; CLOSTRIDIUM BEIJERINCKII; CONTROLLED STUDY; CRISPR CAS SYSTEM; ENZYME ACTIVITY; FERMENTATION; GENE EXPRESSION; GENE REPRESSION; GENETIC TRANSCRIPTION; NONHUMAN; PROMOTER REGION; QUALITATIVE ANALYSIS; STREPTOCOCCUS PYOGENES; BIOSYNTHESIS; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEAT; DOWN REGULATION; EPIGENETIC REPRESSION; GENE EXPRESSION REGULATION; GENETICS; TRANSCRIPTION INITIATION;

AMYLASES; CLOSTRIDIUM BEIJERINCKII; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEATS; CRISPR-ASSOCIATED PROTEINS; DOWN-REGULATION; EPIGENETIC REPRESSION; GENE EXPRESSION REGULATION, BACTERIAL; TRANSCRIPTION, GENETIC; TRANSCRIPTIONAL ACTIVATION;

EID: 84975485770     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.26020     Document Type: Article

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