CO 2 fixation by anaerobic non-photosynthetic mixotrophy for improved carbon conversion

Nature Communications

Volumn 7, Issue , 2016, Pages

  Jones, Shawn W ^a   Fast, Alan G ^b   Carlson, Ellinor D ^b   Wiedel, Carrissa A ^a   Au, Jennifer ^b   Antoniewicz, Maciek R ^b   Papoutsakis, Eleftherios T ^b   Tracy, Bryan P ^a  

^a White Dog Labs Inc   (United States)

^b UNIVERSITY OF DELAWARE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ACETONE; CARBON; CARBON DIOXIDE; SUGAR;

ANOXIC CONDITIONS; BACTERIUM; BIOENGINEERING; CARBON DIOXIDE; CARBON EMISSION; CARBON FIXATION; FERMENTATION; MIXOTROPHY; OPERATIONS TECHNOLOGY; PROCESSING;

ARTICLE; CARBON DIOXIDE FIXATION; CARBON FOOTPRINT; CARBON UTILIZATION; CELL DENSITY; CLOSTRIDIUM; CLOSTRIDIUM LJUNGDAHLII; CONTINUOUS FERMENTATION; FERMENTATION; GAS; MIXOTROPHY;

CLOSTRIDIUM LJUNGDAHLII;

EID: 84990230977     PISSN: None     EISSN: 20411723     Source Type: Journal    
DOI: 10.1038/ncomms12800     Document Type: Article

References (33)

1. Papoutsakis, E. T. Reassessing the progress in the production of advanced biofuels in the current competitive environment and beyond: what are the successes and where progress eludes us and why. Ind. Eng. Chem. Res. 54, 10170-10182 (2015).
2. Atsumi, S., Hanai, T. & Liao, J. C. Non-fermentative pathways for synthesis of branched-chain higher alcohols as biofuels. Nature 451, 86-89 (2008).
3. Bogorad, I. W., Lin, T.-S. & Liao, J. C. Synthetic non-oxidative glycolysis enables complete carbon conservation. Nature 502, 693-697 (2013).
4. Fast, A. G., Schmidt, E. D., Jones, S. W. & Tracy, B. P. Acetogenic mixotrophy: novel options for yield improvement in biofuels and biochemicals production. Curr. Opin. Biotechnol. 33, 60-72 (2015).
5. Fast, A. G. & Papoutsakis, E. T. Stoichiometric and energetic analyses of non-photosynthetic CO2-fixation pathways to support synthetic biology strategies for production of fuels and chemicals. Curr. Opin. Chem. Eng. 1, 380-395 (2012).
6. Ragsdale, S. W. & Pierce, E. Acetogenesis and the Wood-Ljungdahl pathway of CO2 fixation. Biochim. Biophys. Acta 1784, 1873-1898 (2008).
7. Tracy, B. P., Jones, S. W., Fast, A. G., Indurthi, D. C. & Papoutsakis, E. T. Clostridia: the importance of their exceptional substrate and metabolite diversity for biofuel and biorefinery applications. Curr. Opin. Biotechnol. 23, 364-381 (2012).
8. Holladay, J. D., Hu, J., King, D. L. & Wang, Y. An overview of hydrogen production technologies. Catal. Today 139, 244-260 (2009).
9. Li, X. et al. Engineering heterogeneous semiconductors for solar water splitting. J. Mater. Chem. A 3, 2485-2534 (2015).
10. Bertsch, J. & Muller, V. Bioenergetic constraints for conversion of syngas to biofuels in acetogenic bacteria. Biotechnol. Biofuels 8, 210 (2015).
11. Abubackar, H. N., Veiga, M. C. & Kennes, C. Carbon monoxide fermentation to ethanol by Clostridium autoethanogenum in a bioreactor with no accumulation of acetic acid. Bioresour. Technol. 186, 122-127 (2015).
12. Braun, K. & Gottschalk, G. Effect of molecular hydrogen and carbon dioxide on chemo-organotrophic growth of Acetobacterium woodii and Clostridium aceticum. Arch. Microbiol. 128, 294-298 (1981).
13. Liu, C. et al. Influence of glucose fermentation on CO2 assimilation to acetate in homoacetogen Blautia coccoides GA-1. J. Ind. Microbiol. Biotechnol. 42, 1217-1224 (2015).
14. Loubiere, P., Gros, E., Paquet, V. & Lindley, N. D. Kinetics and physiological implications of the growth behavior of Eubacterium limosum on glucose/ methanol mixtures. J. Gen. Microbiol. 138, 979-985 (1992).
15. Kopke, M. et al. Clostridium ljungdahlii represents a microbial production platform based on syngas. Proc. Natl Acad. Sci. USA 107, 13087-13092 (2010).
16. Leang, C., Ueki, T., Nevin, K. P. & Lovley, D. R. A genetic system for Clostridium ljungdahlii: a chassis for autotrophic production of biocommodities and a model homoacetogen. Appl. Environ. Microbiol. 79, 1102-1109 (2013).
17. Nagarajan, H. et al. Characterizing acetogenic metabolism using a genomescale metabolic reconstruction of Clostridium ljungdahlii. Microb. Cell Fact. 12, 118 (2013).
18. Lederle, S. Heterofermentative Acetonproduktion. Ph.D. thesis, Univeristy of Ulm, Germany (2011).
19. Schiel-Bengelsdorf, B. & Durre, P. Pathway engineering and synthetic biology using acetogens. FEBS Lett. 586, 2191-2198 (2012).
20. Hoffmeister, S. et al. Acetone production with metabolically engineered strains of Acetobacterium woodii. Metab. Eng. 36, 37-47 (2016).
21. Banerjee, A., Leang, C., Ueki, T., Nevin, K. P. & Lovley, D. R. Lactose-inducible system for metabolic engineering of Clostridium ljungdahlii. Appl. Environ. Microbiol. 80, 2410-2416 (2014).
22. Kopke, M. et al. Reconstruction of an acetogenic 2,3-butanediol pathway involving a novel NADPH-dependent primary-secondary alcohol dehydrogenase. Appl. Environ. Microbiol. 80, 3394-3403 (2014).
23. Kopke, M. et al. 2,3-Butanediol production by acetogenic bacteria, an alternative route to chemical synthesis, using industrial waste gas. Appl. Environ. Microbiol. 77, 5467-5475 (2011).
24. Meyer, C. L. & Papoutsakis, E. T. Continuous and biomass recycle fermentations of Clostridium acetobutylicum part 1: ATP supply and demand determines product selectivity. Bioprocess Eng. 4, 1-10 (1989).
25. Fontaine, F. E., Peterson, W. H., McCoy, E., Johnson, M. J. & Ritter, G. J. A new type of glucose fermentation by Clostridium thermoaceticum. J. Bacteriol. 43, 701-715 (1942).
26. Tremblay, P. L., Zhang, T., Dar, S. A., Leang, C. & Lovley, D. R. The Rnf complex of Clostridium ljungdahlii is a proton-translocating ferredoxin:NAD+ oxidoreductase essential for autotrophic growth. MBio 4, e00406-e00412 (2012).
27. Ueki, T., Nevin, K. P., Woodard, T. L. & Lovley, D. R. Converting carbon dioxide to butyrate with an engineered strain of Clostridium ljungdahlii. MBio 5, e01636-01614 (2014).
28. Nicolaou, S. A., Gaida, S. M. & Papoutsakis, E. T. A comparative view of metabolite and substrate stress and tolerance in microbial bioprocessing: from biofuels and chemicals, to biocatalysis and bioremediation. Metab. Eng. 12, 307-331 (2010).
29. Au, J., Choi, J., Jones, S. W., Venkataramanan, K. P. & Antoniewicz, M. R. Parallel labeling experiments validate Clostridium acetobutylicum metabolic network model for 13C metabolic flux analysis. Metab. Eng. 26, 23-33 (2014).
30. Tummala, S. B., Welker, N. E. & Papoutsakis, E. T. Development and characterization of a gene expression reporter system for Clostridium acetobutylicum ATCC 824. Appl. Environ. Microbiol. 65, 3793-3799 (1999).
31. Buday, Z., Linden, J. C. & Karim, M. N. Improved acetone-butanol fermentation analysis using subambient HPLC column temperature. Enzyme Microb. Technol. 12, 24-27 (1990).
32. Papoutsakis, E. T. Equations and calculations for fermentations of butyric acid bacteria. Biotechnol. Bioeng. 26, 174-187 (1984).
33. Thauer, R. K., Kaster, A. K., Seedorf, H., Buckel, W. & Hedderich, R. Methanogenic archaea: ecologically relevant differences in energy conservation. Nat. Rev. Microbiol. 6, 579-591 (2008).