Efficient in planta gene targeting in tomato using geminiviral replicons and the CRISPR/Cas9 system

Plant Journal

Volumn 95, Issue 1, 2018, Pages 5-16

  Dahan Meir, Tal ^a   Filler Hayut, Shdema ^a   Melamed Bessudo, Cathy ^a   Bocobza, Samuel ^a,c   Czosnek, Henryk ^b   Aharoni, Asaph ^a   Levy, Avraham A ^a  

^a WEIZMANN INSTITUTE OF SCIENCE   (Israel)

^b HEBREW UNIVERSITY OF JERUSALEM   (Israel)

^c VOLCANI CENTER   (Israel)

Author keywords

CRISPR Cas9; geminiviral replicons; gene replacement; genome editing; homologous recombination

Indexed keywords

BIOCHEMISTRY; FRUITS; GENETIC ENGINEERING; MUTAGENESIS; PIGMENTS; PLANTS (BOTANY); VIRUSES;

CAROTENOID BIOSYNTHESIS; CRISPR-CAS9; DNA DOUBLE STRAND BREAKS; GEMINIVIRAL REPLICONS; GENE REPLACEMENT; HOMOLOGOUS RECOMBINATION; MUTAGENESIS EXPERIMENT; RANDOM MUTAGENESIS;

GENES;

BIOCHEMISTRY; FRUITS; GENES; GENETIC ENGINEERING; VIRUSES;

ALLELE; CRISPR CAS SYSTEM; DOUBLE STRANDED DNA BREAK; GEMINIVIRIDAE; GENE EDITING; GENE TARGETING; GENETICS; HIGH THROUGHPUT SEQUENCING; PLANT GENE; PROCEDURES; REPLICON; TOMATO; TRANSGENIC PLANT;

ALLELES; CRISPR-ASSOCIATED PROTEIN 9; CRISPR-CAS SYSTEMS; DNA BREAKS, DOUBLE-STRANDED; GEMINIVIRIDAE; GENE EDITING; GENE TARGETING; GENES, PLANT; HIGH-THROUGHPUT NUCLEOTIDE SEQUENCING; LYCOPERSICON ESCULENTUM; PLANTS, GENETICALLY MODIFIED; REPLICON;

EID: 85047663477     PISSN: 09607412     EISSN: 1365313X     Source Type: Journal    
DOI: 10.1111/tpj.13932     Document Type: Article

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