Markerless gene knockout and integration to express heterologous biosynthetic gene clusters in Pseudomonas putida

Metabolic Engineering

Volumn 47, Issue , 2018, Pages 463-474

  Choi, Kyeong Rok ^a   Cho, Jae Sung ^a   Cho, In Jin ^a   Park, Dahyeon ^a   Lee, Sang Yup ^a,b  

^a Korea Advanced Institute of Science and Technology (KAIST)   (South Korea)

^b Chalmers University of Technology   (Denmark)

Author keywords

Cre lox; Gene integration; Gene knockout; Pseudomonas putida; RecET; Recombineering

Indexed keywords

BACTERIA; BIOSYNTHESIS; CURING; INTEGRATION; KETONES; METABOLIC ENGINEERING; METABOLISM;

CRE/LOX; GENE KNOCKOUT; PSEUDOMONAS PUTIDA; RECET; RECOMBINEERING;

GENES;

AMINO ACID DERIVATIVE; ANTIBIOTIC AGENT; CRE RECOMBINASE; DNA FRAGMENT; POLYKETIDE; RECT PROTEIN; RECOMBINANT PROTEIN;

ARTICLE; CHROMOSOME; GENE CLUSTER; GENE KNOCKOUT; GENE LOCUS; GENETIC ENGINEERING; HETEROLOGOUS EXPRESSION; HOMOLOGOUS RECOMBINATION; METABOLIC ENGINEERING; NONHUMAN; PLASMID; PRIORITY JOURNAL; PSEUDOMONAS PUTIDA; TRANSPOSON; BIOSYNTHESIS; GENE EXPRESSION; GENETICS; METABOLISM; MULTIGENE FAMILY; PROCEDURES; TRANSGENIC MICROORGANISM;

DNA TRANSPOSABLE ELEMENTS; GENE EXPRESSION; GENETIC ENGINEERING; HOMOLOGOUS RECOMBINATION; MICROORGANISMS, GENETICALLY-MODIFIED; MULTIGENE FAMILY; PSEUDOMONAS PUTIDA; RECOMBINANT PROTEINS;

EID: 85047263606     PISSN: 10967176     EISSN: 10967184     Source Type: Journal    
DOI: 10.1016/j.ymben.2018.05.003     Document Type: Article

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