Heterologous expression and genetic engineering of the tubulysin biosynthetic gene cluster using red/ET recombineering and inactivation mutagenesis

Chemistry and Biology

Volumn 19, Issue 3, 2012, Pages 361-371

  Chai, Yi ^a   Shan, Shiping ^a,b   Weissman, Kira J ^a   Hu, Shengbiao ^a,b   Zhang, Youming ^a   Müller, Rolf ^a  

^a SAARLAND UNIVERSITY   (Germany)

^b HUNAN NORMAL UNIVERSITY   (China)

Author keywords

[No Author keywords available]

Indexed keywords

POLYKETIDE; TUBULYSIN; UNCLASSIFIED DRUG;

ARTICLE; DNA MODIFICATION; GENE CLUSTER; GENE EXPRESSION; GENE INACTIVATION; GENETIC ENGINEERING; GENETIC RECOMBINATION; MUTAGENESIS; MYXOCOCCUS XANTHUS; NONHUMAN; PSEUDOMONAS PUTIDA;

ESCHERICHIA COLI; GENETIC ENGINEERING; MIXED FUNCTION OXYGENASES; MULTIGENE FAMILY; MUTAGENESIS; MYXOCOCCUS XANTHUS; OLIGOPEPTIDES; PROMOTER REGIONS, GENETIC; PSEUDOMONAS PUTIDA;

CYSTOBACTER; MYXOCOCCUS XANTHUS; PSEUDOMONAS PUTIDA;

EID: 84858984727     PISSN: 10745521     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.chembiol.2012.01.007     Document Type: Article

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