Cyanobacteria: Promising biocatalysts for sustainable chemical production

Journal of Biological Chemistry

Volumn 293, Issue 14, 2018, Pages 5044-5052

  Knoot, Cory J ^a   Ungerer, Justin ^a   Wangikar, Pramod P ^b   Pakrasi, Himadri B ^a  

^a WASHINGTON UNIVERSITY   (United States)

^b INDIAN INSTITUTE OF TECHNOLOGY BOMBAY   (India)

Author keywords

[No Author keywords available]

Indexed keywords

ENZYMES; METABOLITES;

CHEMICAL PRODUCTION; DIRECT CONVERSION; END-PRODUCTS; HETEROLOGOUS GENES; MICROBIAL BIOFACTORIES; PHOTOSYNTHETIC PROKARYOTE; SUSTAINABLE PRODUCTION; VALUE ADDED PRODUCTS;

FOSSIL FUELS;

ACETYL COENZYME A; ALCOHOL DERIVATIVE; CARBON DIOXIDE; DIHYDROXYACETONE PHOSPHATE; FATTY ACID; GLYCOGEN; ISOPRENOID; METHYLERYTHRITOL PHOSPHATE; NATURAL PRODUCT; PYRUVIC ACID; SUGAR; UNCLASSIFIED DRUG; BIOFUEL; BIOLOGICAL PRODUCT;

BACTERIAL METABOLISM; BIOCATALYST; BIOTECHNOLOGICAL PRODUCTION; CITRIC ACID CYCLE; CYANOBACTERIUM; GREEN CHEMISTRY; INDUSTRIALIZATION; METABOLIC ENGINEERING; METABOLIC RATE; NONHUMAN; PHOTOAUTOTROPH; PRIORITY JOURNAL; PRODUCTIVITY; REVIEW; BIOCATALYSIS; CHEMISTRY; GENETICS; METABOLIC FLUX ANALYSIS; METABOLISM; MICROBIOLOGY; PHOTOSYNTHESIS; PHYSIOLOGY; PROCEDURES;

BIOCATALYSIS; BIOFUELS; BIOLOGICAL PRODUCTS; CYANOBACTERIA; INDUSTRIAL MICROBIOLOGY; METABOLIC ENGINEERING; METABOLIC FLUX ANALYSIS; PHOTOSYNTHESIS;

EID: 85045076130     PISSN: 00219258     EISSN: 1083351X     Source Type: Journal    
DOI: 10.1074/jbc.R117.815886     Document Type: Review

References (100)

1. Anastas, P. T., and Warner, J. C. (1998) Green Chemistry: Theory and Practice, Oxford University Press, Oxford, UK
2. Whitton, B. A., and Potts, M. (2002) in The Ecology of Cyanobacteria: Their Diversity in Time and Space (Whitton, B. A., and Potts, M., eds) pp. 1–11, Springer, Dordrecht, the Netherlands
3. Hamilton, T. L., Bryant, D. A., and Macalady, J. L. (2016) The role of biology in planetary evolution: cyanobacterial primary production in low-oxygen proterozoic oceans. Environ. Microbiol. 18, 325–340 CrossRef Medline
4. Flombaum, P., Gallegos, J. L., Gordillo, R. A., Rincón, J., Zabala, L. L., Jiao, N., Karl, D. M., Li, W. K., Lomas, M. W., Veneziano, D., Vera, C. S., Vrugt, J. A., and Martiny, A. C. (2013) Present and future global distributions of the marine cyanobacteria Prochlorococcus and Synechococcus. Proc. Natl. Acad. Sci. U.S.A. 110, 9824 –9829 CrossRef Medline
5. Bullerjahn, G. S., and Post, A. F. (2014) Physiology and molecular biology of aquatic cyanobacteria. Front. Microbiol. 5, 359 Medline
6. Hu, Q., Sommerfeld, M., Jarvis, E., Ghirardi, M., Posewitz, M., Seibert, M., and Darzins, A. (2008) Microalgal triacylglycerols as feedstocks for biofuel production: perspectives and advances. Plant J. 54, 621– 639 CrossRef Medline
7. Hankamer, B., Lehr, F., Rupprecht, J., Mussgnug, J. H., Posten, C., and Kruse, O. (2007) Photosynthetic biomass and H2 production by green algae: from bioengineering to bioreactor scale-up. Physiol. Plant 131, 10 –21 CrossRef Medline
8. Dismukes, G. C., Carrieri, D., Bennette, N., Ananyev, G. M., and Posewitz, M. C. (2008) Aquatic phototrophs: efficient alternatives to land-based crops for biofuels. Curr. Opin. Biotechnol. 19, 235–240 CrossRef Medline
9. 2, but a green E. coli. Front. Bioeng. Biotechnol. 2, 36 Medline
10. Chi, Z., O’Fallon, J. V., and Chen, S. (2011) Bicarbonate produced from carbon capture for algae culture. Trends Biotechnol. 29, 537–541 CrossRef Medline
11. Gimpel, J. A., Henríquez, V., and Mayfield, S. P. (2015) In metabolic engineering of eukaryotic microalgae: potential and challenges come with great diversity. Front. Microbiol. 6, 1376 Medline
12. Pate, R., Klise, G., and Wu, B. (2011) Resource demand implications for US algae biofuels production scale-up. Appl. Energy 88, 3377–3388 CrossRef
13. Sharma, N. K., and Stal, L. J. (2014) Cyanobacteria, pp. 167–180 John Wiley & Sons, Ltd., Chichester, UK
14. Markou, G., and Georgakakis, D. (2011) Cultivation of filamentous cyanobacteria (blue-green algae) in agro-industrial wastes and wastewaters: a review. Applied Energy 88, 3389 –3401 CrossRef
15. Deng, M.-D., and Coleman, J. R. (1999) Ethanol synthesis by genetic engineering in cyanobacteria. Appl. Environ. Microbiol. 65, 523–528 Medline
16. Dittmann, E., Gugger, M., Sivonen, K., and Fewer, D. P. (2015) Natural product biosynthetic diversity and comparative genomics of the cyanobacteria. Trends Microbiol. 23, 642– 652 CrossRef Medline
17. Burja, A. M., Banaigs, B., Abou-Mansour, E., Grant Burgess, J., and Wright, P. C. (2001) Marine cyanobacteria—a prolific source of natural products. Tetrahedron 57, 9347–9377 CrossRef
18. Stephanopoulos, G. (1999) Metabolic fluxes and metabolic engineering. Metab. Eng. 1, 1–11 CrossRef Medline
19. Melis, A. (2013) Carbon partitioning in photosynthesis. Curr. Opin. Chem. Biol. 17, 453– 456 CrossRef Medline
20. Lan, E. I., and Liao, J. C. (2011) Metabolic engineering of cyanobacteria for 1-butanol production from carbon dioxide. Metab. Eng. 13, 353–363 CrossRef Medline
21. Lan, E. I., and Liao, J. C. (2012) ATP drives direct photosynthetic production of 1-butanol in cyanobacteria. Proc. Natl. Acad. Sci. U.S.A. 109, 6018 – 6023 CrossRef Medline
22. 2 to 1,2-propanediol. Microb. Cell Fact. 12, 4 CrossRef Medline
23. Angermayr, S. A., Paszota, M., and Hellingwerf, K. J. (2012) Engineering a cyanobacterial cell factory for production of lactic acid. Appl. Environ. Microbiol. 78, 7098 –7106 CrossRef Medline
24. Lassen, L. M., Nielsen, A. Z., Ziersen, B., Gnanasekaran, T., Møller, B. L., and Jensen, P. E. (2014) Redirecting photosynthetic electron flow into light-driven synthesis of alternative products including high-value bioactive natural compounds. ACS Synth. Biol. 3, 1–12 CrossRef Medline
25. Hirokawa, Y., Maki, Y., Tatsuke, T., and Hanai, T. (2016) Cyanobacterial production of 1,3-propanediol directly from carbon dioxide using a synthetic metabolic pathway. Metab. Eng. 34, 97–103 CrossRef Medline
26. Stal, L. J., and Moezelaar, R. (1997) Fermentation in cyanobacteria. FEMS Microbiol. Rev. 21, 179 –211 CrossRef
27. Zhang, S., and Bryant, D. A. (2011) The tricarboxylic acid cycle in cyanobacteria. Science 334, 1551–1553 CrossRef Medline
28. Steinhauser, D., Fernie, A. R., and Araújo, W. L. (2012) Unusual cyanobacterial TCA cycles: not broken just different. Trends Plant Sci. 17, 503–509 CrossRef Medline
29. Anfelt, J., Kaczmarzyk, D., Shabestary, K., Renberg, B., Rockberg, J., Nielsen, J., Uhlén, M., and Hudson, E. P. (2015) Genetic and nutrient modulation of acetyl-CoA levels in Synechocystis for n-butanol production. Microb. Cell Fact. 14, 167 CrossRef Medline
30. Ruffing, A. M. (2014) Improved free fatty acid production in cyanobacteria with Synechococcus sp. PCC 7002 as host. Front. Bioeng. Biotechnol. 2, 17 Medline
31. Song, K., Tan, X., Liang, Y., and Lu, X. (2016) The potential of Synechococcus elongatus UTEX 2973 for sugar feedstock production. Appl. Microbiol. Biotechnol. 100, 7865–7875 CrossRef Medline
32. Hendry, J. I., Prasannan, C. B., Joshi, A., Dasgupta, S., and Wangikar, P. P. (2016) Metabolic model of Synechococcus sp. PCC 7002: Prediction of flux distribution and network modification for enhanced biofuel production. Bioresour. Technol. 213, 190 –197 CrossRef Medline
33. 13C flux analysis. Metab. Eng. 13, 656 – 665 CrossRef Medline
34. 13C flux analysis of cyanobacterial isobutyraldehyde production. Metab. Eng. 42, 9 –18 CrossRef Medline
35. 13C metabolic flux analysis. Biotechnol. Bioeng. 114, 2298 –2308 CrossRef Medline
36. 2 conversion to ethylene. Nat. Plants 1, 15053 CrossRef
37. Atsumi, S., Higashide, W., and Liao, J. C. (2009) Direct photosynthetic recycling of carbon dioxide to isobutyraldehyde. Nat. Biotechnol. 27, 1177–1180 CrossRef Medline
38. Oliver, J. W., Machado, I. M., Yoneda, H., and Atsumi, S. (2013) Cyanobacterial conversion of carbon dioxide to 2,3-butanediol. Proc. Natl. Acad. Sci. U.S.A. 110, 1249 –1254 CrossRef Medline
39. Hirokawa, Y., Maki, Y., and Hanai, T. (2017) Improvement of 1,3-pro-panediol production using an engineered cyanobacterium, Synechococcus elongatus by optimization of the gene expression level of a synthetic metabolic pathway and production conditions. Metab. Eng. 39, 192–199 CrossRef Medline
40. 2 in cyanobacterium Synechococcus elongatus PCC 7942 and characterization of the native acetohydroxyacid synthase. Energy Environ. Sci. 5, 9574 –9583 CrossRef
41. Kopka, J., Schmidt, S., Dethloff, F., Pade, N., Berendt, S., Schottkowski, M., Martin, N., Dühring, U., Kuchmina, E., Enke, H., Kramer, D., Wilde, A., Hagemann, M., and Friedrich, A. (2017) Systems analysis of ethanol production in the genetically engineered cyanobacterium Synechococcus sp. PCC 7002. Biotechnol. Biofuels 10, 56 CrossRef Medline
42. Niederholtmeyer, H., Wolfstädter, B. T., Savage, D. F., Silver, P. A., and Way, J. C. (2010) Engineering cyanobacteria to synthesize and export hydrophilic products. Appl. Environ. Microbiol. 76, 3462–3466 CrossRef Medline
43. Li, X., Shen, C. R., and Liao, J. C. (2014) Isobutanol production as an alternative metabolic sink to rescue the growth deficiency of the glycogen mutant of Synechococcus elongatus PCC 7942. Photosynth. Res. 120, 301–310 CrossRef Medline
44. Oliver, J. W., and Atsumi, S. (2015) A carbon sink pathway increases carbon productivity in cyanobacteria. Metab. Eng. 29, 106 –112 CrossRef Medline
45. 2 by genetically engineered cyanobacteria. Green Chem. 17, 3100 –3110 CrossRef
46. Hirokawa, Y., Suzuki, I., and Hanai, T. (2015) Optimization of isopropanol production by engineered cyanobacteria with a synthetic metabolic pathway. J. Biosci. Bioeng. 119, 585–590 CrossRef Medline
47. Kusakabe, T., Tatsuke, T., Tsuruno, K., Hirokawa, Y., Atsumi, S., Liao, J. C., and Hanai, T. (2013) Engineering a synthetic pathway in cyanobacteria for isopropanol production directly from carbon dioxide and light. Metab. Eng. 20, 101–108 CrossRef Medline
48. 2. Metab. Eng. 16, 68 –77 CrossRef Medline
49. Liu, X., Sheng, J., and Curtiss, R., 3rd (2011) Fatty acid production in genetically modified cyanobacteria. Proc. Natl. Acad. Sci. U.S.A. 108, 6899 – 6904 CrossRef Medline
50. Zhou, J., Zhang, H., Zhang, Y., Li, Y., and Ma, Y. (2012) Designing and creating a modularized synthetic pathway in cyanobacterium Synechocystis enables production of acetone from carbon dioxide. Metab. Eng. 14, 394 – 400 CrossRef Medline
51. Wang, W., Liu, X., and Lu, X. (2013) Engineering cyanobacteria to improve photosynthetic production of alka (e) nes. Biotechnol. Biofuels 6, 69 CrossRef Medline
52. 2 in cyanobacterium Synechocystis sp. PCC 6803. Metab. Eng. 34, 60 –70 CrossRef Medline
53. 2 in Synechococcus elongatus PCC 7942 under light and aerobic condition. Plant Biotechnol. J. 14, 1768 –1776 CrossRef Medline
54. 2 using Synechococcus elongatus PCC 7942. Metab. Eng. 31, 163–170 CrossRef Medline
55. 2 to ethylene in recombinant cyanobacterium Synechocystis 6803. Energy Environ. Sci. 5, 8998 –9006 CrossRef
56. Lan, E. I., and Wei, C. T. (2016) Metabolic engineering of cyanobacteria for the photosynthetic production of succinate. Metab. Eng. 38, 483– 493 CrossRef Medline
57. McNeely, K., Xu, Y., Bennette, N., Bryant, D. A., and Dismukes, G. C. (2010) Redirecting reductant flux into hydrogen production via metabolic engineering of fermentative carbon metabolism in a cyanobacterium. Appl. Environ. Microbiol. 76, 5032–5038 CrossRef Medline
58. Tiruveedula, G. S. S., and Wangikar, P. P. (2017) Gene essentiality, conservation index and co-evolution of genes in cyanobacteria. PLoS ONE 12, e0178565 CrossRef Medline
59. Aikawa, S., Nishida, A., Ho, S.-H., Chang, J.-S., Hasunuma, T., and Kondo, A. (2014) Glycogen production for biofuels by the euryhaline cyanobacteria Synechococcus sp. strain PCC 7002 from an oceanic environment. Biotechnol. Biofuels 7, 88 CrossRef Medline
60. Blumwald, E., Mehlhorn, R. J., and Packer, L. (1983) Ionic osmoregulation during salt adaptation of the cyanobacterium Synechococcus 6311. Plant Physiol. 73, 377–380 CrossRef Medline
61. Ducat, D. C., and Silver, P. A. (2012) Improving carbon fixation pathways. Curr. Opin. Chem. Biol. 16, 337–344 CrossRef Medline
62. 2 in a cyanobacterium. Metab. Eng. 21, 60 –70 CrossRef Medline
63. Davies, F. K., Work, V. H., Beliaev, A. S., and Posewitz, M. C. (2014) Engineering limonene and bisabolene production in wild type and a glycogen-deficient mutant of Synechococcus sp. PCC 7002. Front. Bioeng. Biotechnol. 2, 21 Medline
64. Ducat, D. C., Avelar-Rivas, J. A., Way, J. C., and Silver, P. A. (2012) Rerouting carbon flux to enhance photosynthetic productivity. Appl. Environ. Microbiol. 78, 2660 –2668 CrossRef Medline
65. Rock, C. O., and Jackowski, S. (2002) Forty years of bacterial fatty acid synthesis. Biochem. Biophys. Res. Commun. 292, 1155–1166 CrossRef Medline
66. Winters, K., Parker, P. L., and van Baalen, C. (1969) Hydrocarbons of blue-green algae: geochemical significance. Science 163, 467– 468 CrossRef Medline
67. Schirmer, A., Rude, M. A., Li, X., Popova, E., and del Cardayre, S. B. (2010) Microbial biosynthesis of alkanes. Science 329, 559 –562 CrossRef Medline
68. Mendez-Perez, D., Begemann, M. B., and Pfleger, B. F. (2011) Modular synthase-encoding gene involved in -olefin biosynthesis in Synechococcus sp. strain PCC 7002. Appl. Environ. Microbiol. 77, 4264 – 4267 CrossRef Medline
69. Kaczmarzyk, D., and Fulda, M. (2010) Fatty acid activation in cyanobacteria mediated by acyl-acyl carrier protein synthetase enables fatty acid recycling. Plant Physiol. 152, 1598 –1610 CrossRef Medline
70. Ruffing, A. M. (2013) RNA-Seq analysis and targeted mutagenesis for improved free fatty acid production in an engineered cyanobacterium. Biotechnol. Biofuels 6, 113 CrossRef Medline
71. Ruffing, A. M. (2013) Borrowing genes from Chlamydomonas reinhardtii for free fatty acid production in engineered cyanobacteria. J. Appl. Phycol. 25, 1495–1507 CrossRef
72. Tan, X., Yao, L., Gao, Q., Wang, W., Qi, F., and Lu, X. (2011) Photosynthesis driven conversion of carbon dioxide to fatty alcohols and hydrocarbons in cyanobacteria. Metab. Eng. 13, 169–176 CrossRef Medline
73. Kaiser, B. K., Carleton, M., Hickman, J. W., Miller, C., Lawson, D., Budde, M., Warrener, P., Paredes, A., Mullapudi, S., Navarro, P., Cross, F., and Roberts, J. M. (2013) Fatty aldehydes in cyanobacteria are a metabolically flexible precursor for a diversity of biofuel products. PLoS ONE 8, e58307 CrossRef Medline
74. Kageyama, H., Waditee-Sirisattha, R., Sirisattha, S., Tanaka, Y., Ma-hakhant, A., and Takabe, T. (2015) Improved alkane production in nitrogen-fixing and halotolerant cyanobacteria via abiotic stresses and genetic manipulation of alkane synthetic genes. Curr. Microbiol. 71, 115–120 CrossRef Medline
75. Jiménez-Díaz, L., Caballero, A., Pérez-Hernandez, N., and Segura, A. (2017) Microbial alkane production for jet fuel industry: Motivation, state of the art and perspectives. Microb. Biotechnol. 10, 103–124 Medline
76. Ruffing, A. M., and Jones, H. D. (2012) Physiological effects of free fatty acid production in genetically engineered Synechococcus elongatus PCC 7942. Biotechnol. Bioeng. 109, 2190 –2199 CrossRef Medline
77. 2-limitation-inducible green recovery of fatty acids from cyanobacterial biomass. Proc. Natl. Acad. Sci. U.S.A. 108, 6905– 6908 CrossRef Medline
78. Lindberg, P., Park, S., and Melis, A. (2010) Engineering a platform for photosynthetic isoprene production in cyanobacteria, using Synechocystis as the model organism. Metab. Eng. 12, 70 –79 CrossRef Medline
79. Englund, E., Andersen-Ranberg, J., Miao, R., Hamberger, B., and Lindberg, P. (2015) Metabolic engineering of Synechocystis sp. PCC 6803 for production of the plant diterpenoid manoyl oxide. ACS Synth. Biol. 4, 1270 –1278 CrossRef Medline
80. 2. Energy Environ. Sci. 9, 1400 –1411 CrossRef
81. Wang, X., Liu, W., Xin, C., Zheng, Y., Cheng, Y., Sun, S., Li, R., Zhu, X.-G., Dai, S. Y., Rentzepis, P. M., and Yuan, J. S. (2016) Enhanced limonene production in cyanobacteria reveals photosynthesis limitations. Proc. Natl. Acad. Sci. U.S.A. 113, 14225–14230 CrossRef Medline
82. Bentley, F. K., García-Cerdán, J. G., Chen, H.-C., and Melis, A. (2013) Paradigm of monoterpene (-phellandrene) hydrocarbons production via photosynthesis in cyanobacteria. Bioenergy Res. 6, 917–929 CrossRef
83. Formighieri, C., and Melis, A. (2015) A phycocyanin-phellandrene synthase fusion enhances recombinant protein expression and -phellan-drene (monoterpene) hydrocarbons production in Synechocystis (cyanobacteria). Metab. Eng. 32, 116 –124 CrossRef Medline
84. Reinsvold, R. E., Jinkerson, R. E., Radakovits, R., Posewitz, M. C., and Basu, C. (2011) The production of the sesquiterpene -caryophyllene in a transgenic strain of the cyanobacterium Synechocystis. J. Plant Physiol. 168, 848 – 852 CrossRef Medline
85. Englund, E., Pattanaik, B., Ubhayasekera, S. J., Stensjö, K., Bergquist, J., and Lindberg, P. (2014) Production of squalene in Synechocystis sp. PCC 6803. PLoS ONE 9, e90270 CrossRef Medline
86. 2, water, and light into the long-chain hydrocarbon farnesene. Appl. Microbiol. Biotechnol. 98, 9869–9877 CrossRef Medline
87. Marienhagen, J., and Bott, M. (2013) Metabolic engineering of microorganisms for the synthesis of plant natural products. J. Biotechnol. 163, 166 –178 CrossRef Medline
88. Leonard, E., Runguphan, W., O’Connor, S., and Prather, K. J. (2009) Opportunities in metabolic engineering to facilitate scalable alkaloid production. Nat. Chem. Biol. 5, 292–300 CrossRef Medline
89. Newman, D. J., and Cragg, G. M. (2016) Natural products as sources of new drugs from 1981 to 2014. J. Nat. Prod. 79, 629 – 661 CrossRef Medline
90. Fischbach, M. A., and Walsh, C. T. (2006) Assembly-line enzymology for polyketide and nonribosomal peptide antibiotics: logic, machinery, and mechanisms. Chem. Rev. 106, 3468 –3496 CrossRef Medline
91. Kehr, J. C., Gatte Picchi, D., and Dittmann, E. (2011) Natural product biosyntheses in cyanobacteria: a treasure trove of unique enzymes. Beil-stein J. Org. Chem. 7, 1622–1635 CrossRef Medline
92. Shao, Z., Zhao, H., and Zhao, H. (2009) DNA assembler, an in vivo genetic method for rapid construction of biochemical pathways. Nucleic Acids Res. 37, e16 CrossRef Medline
93. Trauger, J. W., and Walsh, C. T. (2000) Heterologous expression in Escherichia coli of the first module of the nonribosomal peptide synthetase for chloroeremomycin, a vancomycin-type glycopeptide antibiotic. Proc. Natl. Acad. Sci. U.S.A. 97, 3112–3117 CrossRef Medline
94. Ziemert, N., Ishida, K., Liaimer, A., Hertweck, C., and Dittmann, E. (2008) Ribosomal synthesis of tricyclic depsipeptides in bloom-forming cyanobacteria. Angew. Chem. Int. Ed. Engl. 47, 7756 –7759 CrossRef Medline
95. Jones, A. C., Ottilie, S., Eustáquio, A. S., Edwards, D. J., Gerwick, L., Moore, B. S., and Gerwick, W. H. (2012) Evaluation of Streptomyces coelicolor A3(2) as a heterologous expression host for the cyanobacterial protein kinase C activator lyngbyatoxin A. FEBS J. 279, 1243–1251 CrossRef Medline
96. Chang, M. C., Eachus, R. A., Trieu, W., Ro, D.-K., and Keasling, J. D. (2007) Engineering Escherichia coli for production of functionalized terpenoids using plant P450s. Nat. Chem. Biol. 3, 274 –277 CrossRef Medline
97. Lassen, L. M., Nielsen, A. Z., Olsen, C. E., Bialek, W., Jensen, K., Møller, B. L., and Jensen, P. E. (2014) Anchoring a plant cytochrome P450 via PsaM to the thylakoids in Synechococcus sp. PCC 7002: Evidence for light-driven biosynthesis. PLoS ONE 9, e102184 CrossRef Medline
98. Xue, Y., Zhang, Y., Grace, S., and He, Q. (2014) Functional expression of an Arabidopsis p450 enzyme, p-coumarate-3-hydroxylase, in the cyanobacterium Synechocystis PCC 6803 for the biosynthesis of caffeic acid. J. Appl. Phycol. 26, 219 –226 CrossRef
99. Wlodarczyk, A., Gnanasekaran, T., Nielsen, A. Z., Zulu, N. N., Mellor, S. B., Luckner, M., Thøfner, J. F., Olsen, C. E., Mottawie, M. S., Burow, M., Pribil, M., Feussner, I., Møller, B. L., and Jensen, P. E. (2016) Metabolic engineering of light-driven cytochrome P450 dependent pathways into Synechocystis sp. PCC 6803. Metab. Eng. 33, 1–11 CrossRef Medline
100. Videau, P., Wells, K. N., Singh, A. J., Gerwick, W. H., and Philmus, B. (2016) Assessment of Anabaena sp. strain PCC 7120 as a heterologous expression host for cyanobacterial natural products: production of Lyn-gbyatoxin A. ACS Synth. Biol. 5, 978 –988 CrossRef Medline