Optimization of isopropanol production by engineered cyanobacteria with a synthetic metabolic pathway

Journal of Bioscience and Bioengineering

Volumn 119, Issue 5, 2015, Pages 585-590

  Hirokawa, Yasutaka ^a   Suzuki, Iwane ^b   Hanai, Taizo ^a  

^a KYUSHU UNIVERSITY   (Japan)

^b UNIVERSITY OF TSUKUBA   (Japan)

Author keywords

Acetate utilization; Cyanobacteria; Dark and anaerobic condition; Isopropanol production; Synthetic metabolic pathway

Indexed keywords

CARBON DIOXIDE; METABOLISM; SOLAR ENERGY;

ANAEROBIC CONDITIONS; CYANOBACTERIA; GROWTH CONDITIONS; ISO-PROPANOLS; METABOLIC PATHWAYS; OPTIMIZED PRODUCTION; PRODUCTION MEDIUM; SYNECHOCOCCUS ELONGATUS;

ALTERNATIVE FUELS;

2 PROPANOL; ACETIC ACID; ACETIC ACID DERIVATIVE; BUFFER;

AEROBIC METABOLISM; ALCOHOL PRODUCTION; ANAEROBIC METABOLISM; ARTICLE; BACTERIAL CELL; BACTERIAL GROWTH; BACTERIAL METABOLISM; BACTERIAL STRAIN; CHEMICAL COMPOSITION; CULTURE MEDIUM; DARKNESS; GROWTH CURVE; LIGHT; METABOLIC ENGINEERING; MOLECULAR CLONING; NONHUMAN; PH; PRODUCTIVITY; SYNECHOCOCCUS ELONGATUS; ANAEROBIC GROWTH; CYANOBACTERIUM; GENETICS; GROWTH, DEVELOPMENT AND AGING; METABOLISM; RADIATION RESPONSE;

CYANOBACTERIA; SYNECHOCOCCUS ELONGATUS PCC 7942;

2-PROPANOL; ACETATES; AEROBIOSIS; ANAEROBIOSIS; BUFFERS; CYANOBACTERIA; DARKNESS; HYDROGEN-ION CONCENTRATION; LIGHT; METABOLIC ENGINEERING; METABOLIC NETWORKS AND PATHWAYS;

EID: 84925128834     PISSN: 13891723     EISSN: 13474421     Source Type: Journal    
DOI: 10.1016/j.jbiosc.2014.10.005     Document Type: Article

References (29)

1. McEwen J.T., Atsumi S. Alternative biofuel production in non-natural hosts. Curr. Opin. Biotechnol. 2012, 23:744-750.
2. Zhang F., Rodriguez S., Keasling J.D. Metabolic engineering of microbial pathways for advanced biofuels production. Curr. Opin. Biotechnol. 2011, 22:775-783.
3. Koksharova O.A., Wolk C.P. Genetic tools for cyanobacteria. Appl. Microbiol. Biotechnol. 2002, 58:123-137.
4. Clerico E.M., Ditty J.L., Golden S.S. Specialized techniques for site-directed mutagenesis in cyanobacteria. Circadian rhythms 2007, 155-171. Humana Press Inc., New Jersey. E. Rosato (Ed.).
5. Kotani H., Kaneko T., Matsubayashi T., Sato S., Sugiura M., Tabata S. Aphysical map of the genome of a unicellular cyanobacterium Synechocystis sp. strain PCC6803. DNA Res. 1994, 1:303-307.
6. Holtman C.K., Chen Y., Sandoval P., Gonzales A., Nalty M.S., Thomas T.L., Youderian P., Golden S.S. High-throughput functional analysis of the Synechococcus elongatus PCC 7942 genome. DNA Res. 2005, 12:103-115.
7. Rosegaard L., Porcellinis A.J., Jacobsen J.H., Frigaard N.U., Sakuragi Y. Bioengineering of carbon fixation, biofuels, and biochemical in cyanobacteria and plants. J.Biotechnol. 2012, 162:134-147.
8. Wang B., Wang J., Zhang W., Meldrum D.R. Application of synthetic biology in cyanobacteria and algae. Front. Microbiol. 2012, 3:344.
9. Yu Y., You L., Liu D., Hollinshead W., Tang Y.J., Zhang F. Development of Synechocystis sp. PCC 6803 as a phototropic cell factory. Mar. Drugs 2013, 11:2894-2916.
10. Kusakabe T., Tatsuke T., Tsuruno K., Hirokawa T., Atsumi S., Liao J.C., Hanai T. Engineering a synthetic pathway in cyanobacteria for isopropanol production directly from carbon dioxide and light. Metab. Eng. 2013, 20:101-108.
11. Kibby C.L., Hall W.K. Studies of acid catalyzed reactions. XII. Alcohol decomposition over hydroxyapatite catalysts. J.Catal. 1972, 29:144-159.
12. Molenda J. The oil and petrochemical industries are facing process changes consequent upon the expected propylene demand rise. Przem. Chem. 2004, 83:320-324.
13. Hanai T., Atsumi S., Liao J.C. Engineered synthetic pathway for isopropanol production in Escherichia coli. Appl. Environ. Microbiol. 2007, 73:7814-7818.
14. Alagesan S., Gaudana S.B., Sinha A., Wangikar P.P. Metabolic flux analysis of Cyanothece sp. ATCC 51142 under mixotrophic conditions. Photosynth. Res. 2013, 118:191-198.
15. 13C flux analysis. Metab. Eng. 2011, 13:656-665.
16. Zhou J., Zhand H., Zhang Y., Li Y., Ma Y. Designing and creating a modularized synthetic pathway in cyanobacterium Synechocystis enables production of acetone from carbon dioxide. Metab. Eng. 2012, 14:394-400.
17. Lan E.I., Liao J.C. Metabolic engineering of cyanobacteria for 1-butanol production from carbon dioxide. Metab. Eng. 2011, 13:353-363.
18. Stal L.J., Moezelaar R. Fermentation in cyanobacteria. FEMS Microbiol. Rev. 1997, 21:179-211.
19. Oliver J.W.K., Machado I.M.P., Yoneda H., Atsumi S. Combinatorial optimization of cyanobacterial 2,3-butanediol production. Metab. Eng. 2014, 22:76-82.
20. Dexter J., Fu P. Metabolic engineering of cyanobacteria for ethanol production. Energy Environ. Sci. 2009, 2:857-864.
21. Andersson C.R., Tsinoremas N.F., Shelton J., Lebedeva N.V., Yarrow J., Min H.T., Goldem S.S. Application of bioluminescence to the study of circadian rhythms in cyanobacteria. Methods Enzymol. 2000, 305:527-542.
22. Bustos S.A., Golden S.S. Expression of the psbDII gene in Synechococcus sp. strain-PCC 7942 requires sequences downstream of the transcription start site. J.Bacteriol. 1991, 173:7525-7533.
23. Suzuki E., Ohkawa H., Moriya K., Matsubara T., Nagaike Y., Iwasaki I., Fujiwara S., Tsuzuki M., Nakamura Y. Carbohydrate metabolism in mutants of the cyanobacterium Synechococcus elongatus PCC 7942 defective in glycogen synthesis. Appl. Environ. Microbiol. 2010, 76:3153-3159.
24. Monshupanee T., Incharoensakdi A. Enhanced accumulation of glycogen, lipids and polyhydroxybutyrate under optimal nutrients and light intensities in the cyanobacterium Synechocystis sp. PCC 6803. J.Appl. Microbiol. 2013, 116:830-838.
25. Panda B., Mallick N. Enhanced poly-β-hydroxybutyrate accumulation in a unicellular cyanobacterium, Synechocystis sp. PCC 6803. Lett. Appl. Microbiol. 2006, 44:194-198.
26. 2. Metab. Eng. 2013, 16:68-77.
27. Takahashi H., Miyake M., Tokiwa Y., Asada Y. Improved accumulation of poly-3-hydroxybutyrate by a recombinant cyanobacterium. Biotechnol. Lett. 1998, 20:183-186.
28. Sharma L., Mallick N. Accumulation of poly-β-hydroxybutyrate in Nostoc muscorum: regulation by pH, light-dark cycles, N and P status and carbon source. Bioresour. Technol. 2005, 96:1304-1310.
29. Ananyev G.M., Skizim N.J., Dismukes G.C. Enhancing biological hydrogen production from cyanobacteria by removal of excreted products. J.Biotechnol. 2012, 162:97-104.