Engineering a synthetic pathway in cyanobacteria for isopropanol production directly from carbon dioxide and light

Metabolic Engineering

Volumn 20, Issue , 2013, Pages 101-108

  Kusakabe, Tamami ^a   Tatsuke, Tsuneyuki ^a   Tsuruno, Keigo ^a   Hirokawa, Yasutaka ^a   Atsumi, Shota ^b   Liao, James C ^c   Hanai, Taizo ^a  

^a KYUSHU UNIVERSITY   (Japan)

^b UNIVERSITY OF CALIFORNIA   (United States)

^c UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Cyanobacteria; Isopropanol production; Synthetic pathway

Indexed keywords

CLOSTRIDIUM; ESCHERICHIA COLI; SOLAR ENERGY;

CLOSTRIDIUM ACETOBUTYLICUM ATCC 824; CLOSTRIDIUM BEIJERINCKII; CYANOBACTERIA; ESCHERICHIA COLI K-12; ISO-PROPANOLS; SECONDARY ALCOHOL DEHYDROGENASE; SYNECHOCOCCUS ELONGATUS; SYNTHETIC PATHWAYS;

CARBON DIOXIDE;

2 PROPANOL; ACETIC ACID; ACETOACETATE DECARBOXYLASE; ACETONE; ACETYL COENZYME A ACETYLTRANSFERASE; ALCOHOL DEHYDROGENASE; CARBON DIOXIDE; CARBOXYLYASE; COENZYME A TRANSFERASE; UNCLASSIFIED DRUG;

ARTICLE; BACTERIAL STRAIN; CLOSTRIDIUM ACETOBUTYLICUM; CLOSTRIDIUM BEIJERINCKII; CONTROLLED STUDY; ESCHERICHIA COLI K 12; GENOME; GROWTH INHIBITION; LIGHT; METABOLIC ENGINEERING; NONHUMAN; PRIORITY JOURNAL; SYNECHOCOCCUS ELONGATUS;

CLOSTRIDIUM ACETOBUTYLICUM ATCC 824; CLOSTRIDIUM BEIJERINCKII; CYANOBACTERIA; ESCHERICHIA COLI K12; SYNECHOCOCCUS ELONGATUS;

CYANOBACTERIA; ISOPROPANOL PRODUCTION; SYNTHETIC PATHWAY;

2-PROPANOL; BACTERIAL PROTEINS; CARBON DIOXIDE; CLOSTRIDIUM ACETOBUTYLICUM; CLOSTRIDIUM BEIJERINCKII; ESCHERICHIA COLI K12; LIGHT; METABOLIC ENGINEERING; RECOMBINANT PROTEINS; SYNECHOCOCCUS;

EID: 84885166683     PISSN: 10967176     EISSN: 10967184     Source Type: Journal    
DOI: 10.1016/j.ymben.2013.09.007     Document Type: Article

References (44)

1. Andersson C.R., Tsinoremas N.F., Shelton J., Lebedeva N.V., Yarrow J., Min H.T., Golden S.S. Application of bioluminescence to the study of circadian rhythms in cyanobacteria. Methods Enzymol. 2000, 305:527-542.
2. Atsumi S., Higashide W., Laio J.C. Direct photosynthetic recycling of carbon dioxide to isobutyraldehyde. Nat. Biotechnol. 2009, 27:1177-1180.
3. Baba T., Ara T., Hasegawa M., Takai Y., Okumura Y., Baba M., Datsenko K.A., Tomita M., Wanner B.L., Mori H. Construction of Escherichia coli K-12 in-frame, single-gene knock-out mutants-the Keio collection. Mol. Syst. Biol. 2006, 2, 2006, 2006.0008. 10.1038/msb4100050.
4. Bustos S.A., Golden S.S. Expression of the psbDII gene in Synechococcus sp Strain-PCC 7942 requires sequences downstream of the transcription start site. J. Bacteriol. 1991, 173:7525-7533.
5. Chen J.S., Hiu S.F. Acetone-butanol-isopropanol production by Clostridium beijerinckii (synonym, Clostridium butylicum). Biotechnol. Lett. 1986, 8:371-376.
6. Chen J.S. Alcohol dehydrogenase: multiplicity and relatedness in the solvent-producing clostridia. FEMS Microbiol. Rev. 1995, 17:263-273.
7. Choi Y.J., Park J.H., Kim T.Y., Lee S.Y. Metabolic engineering of Escherichia coli for the production of 1-propanol. Metab. Eng. 2012, 14:477-486.
8. Curran K.A., Alper H.S. Expanding the chemical palate of cells by combining systems biology and metabolic engineering. Metab. Eng. 2012, 14:289-297.
9. Ducat D.C., Sachdeva G., Silver P.A. Rewiring hydrogenase-dependent redox circuits in cyanobacteria. Proc. Natl. Acad. Sci. USA 2011, 108:3941-3946.
10. Foster T.J., Davis M.A., Robert D.E., Takeshita K., Kleckner N. Genetic organization of Transposon Tn10. Cell 1981, 23:201-213.
11. Gao Z., Zhao H., Li Z., Tan X., Lu X. Photosynthetic production of ethanol from carbon dioxide in genetically engineered cyanobacteria. Energy Environ. Sci. 2012, 5:9857-9865.
12. Geerts D., Bovy A., Vrieze G., Borrias M., Weisbeek P. Inducible expression of heterologous genes targeted to a chromosomal platform in the cyanobacterium Synechococcus sp. PCC7942. Microbiology 1995, 141:831-841.
13. Hanai T., Atsumi S., Liao J.C. Engineered synthetic pathway for isopropanol production in Escherichia coli. Appl. Environ. Microbiol. 2007, 73:7814-7818.
14. Huang H.H., Camsund D., Lindblad P., Heidorn T. Design and characterization of molecular tools for synthetic biology approach towards developing cyanobacterial biotechnology. Nucl. Acids Res. 2010, 38:2577-2593.
15. Inokuma K., Liao J.C., Okamoto M., Hanai T. Improvement of isopropanol production by metabolically engineered Escherichia coli using gas stripping. J. Biosci. Bioeng. 2010, 110:696-701.
16. Ismaiel A.A., Zhu C.X., Colby G.D., Chen J.S. Purification and characterization of a primary-secondary alcohol dehydrogenase from two strains of Clostridium beijerinckii. J. Bacteriol. 1993, 175:5097-5105.
17. Jenkins L.S., Nunn W.D. Genetic molecular characterization of the genes involved in short-chain fatty acid degradation in Escherichia coli: the ato system. J. Bacteriol. 1987, 169:42-52.
18. Kibby C.L., Hall W.K. Studies of acid catalyzed reactions. XII. Alcohol decomposition over hydroxyapatite catalysts. J. Catal. 1972, 29:144-159.
19. Lamed R.J., Zeikus J.G. Novel NADP-linked alcohol-aldehyde/ketone oxidoreductase in thermophilic ethanologenic bacteria. Biochem. J. 1981, 195:183-190.
20. Lan E.I., Liao J.C. Metabolic engineering of cyanobacteria for 1-butanol production from carbon dioxide. Metab. Eng. 2011, 13:353-363.
21. Lan E.I., Liao J.C. ATP drives direct photosynthetic production of 1-butanol in cyanobacteria. Proc. Natl. Acad. Sci. USA 2012, 109:6018-6023.
22. Lan E.I., Ro S., Liao J.C. Oxygen-tolerant Coenzyme A-acylating aldehyde dehydrogenase facilitates efficient photosynthetic n-butanol biosynthesis in cyanobacteria. Energy Environ. Sci. 2013, 6:2672-2681.
23. Lindberg P., Park S., Melis A. Engineering a platform for photosynthetic isoprene production in cyanobacteria, using Synechocystis as the model organism. Metab. Eng. 2010, 12:70-79.
24. Liu X., Sheng J., Curtiss R. Fatty acid production in genetically modified cyanobacteria. Proc. Natl. Acad. Sci. USA 2011, 108:6899-6904.
25. Lutz R., Bujard H. Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, TetR/O and AraC/I1-I2 regulatory elelments. Nucl. Acids Res. 1997, 25:1203-1210.
26. Machado I.M.P., Atsumi S. Cyanobacterial biofuel production. J. Biotechnol. 2012, 162:50-56.
27. Miyake M., Erata M., Asada Y. A thermophilic cyanobacterium, Synechococcus sp. MA19, capable of accumulating poly-β-hydroxybutyrate. J. Ferment. Bioeng. 1996, 82:512-514.
28. Molenda J. The oil and petrochemical industries are facing process changes consequent upon the expected propylene demand rise. Przem. Chem. 2004, 83:320-324.
29. Nicolaou S.A., Gaida S.M., Papoutsakis E.T. A comparative view of metabolite and substrate stress and tolerance in microbial bioprocessing: from biofuels and chemicals, to biocatalysis and bioremediation. Metab. Eng. 2010, 12:307-331.
30. Nishioka M., Nakai K., Miyake M., Asada Y., Taya M. Production of poly-β-hydroxybutyrate by thermophilic cyanobacterium, Synechococcus sp. MA19, under phosphate-limited conditions. Biotechnol. Lett. 2001, 23:1095-1099.
31. Oliver J.W.K., Machado I.M.P., Yoneda H., Atsumi S. Cyanobacterial conversion of carbon dioxide to 2, 3-butanediol. Proc. Natl. Acad. Sci. USA 2013, 110:1249-1254.
32. Osburn O.L., Brown R.W., Werkman C.H. The butyl alcohol-isopropyl alcohol fermentation. J. Biol. Chem. 1937, 121:685-695.
33. Petersen D.J., Bennett.G.N. Purification of acetoacetate decarboxylase from Clostridium acetobutylicum ATCC 824 and cloning of acetoacetate decarboxylase gene in Escherichia coli. Appl. Environ. Microbiol. 1990, 56:3491-3498.
34. 2 in cyanobacteria Synechococcus elongatus PCC7942 and characterization of the native acetohydroxyacid synthase. Energy Environ. Sci. 2013, 5:9574-9583.
35. Soma Y., Inokuma K., Tanaka T., Ogino C., Kondo A., Okamoto M., Hanai T. Direct isopropanol production from cellobiose by engineered Escherichia coli using a synthetic pathway and a cell surface display system. J. Biosci. Bioeng. 2012, 114:80-85.
36. Sramek S.J., Frerman F.E. Steady state kinetic mechanism of the Escherichia coli coenzyme A transferase. Arch. Biochem. Biophys. 1977, 181:178-184.
37. Stal L.J., Moezelaar R. Fermentation in cyanobacteria. FEMS Microbiol. Rev. 1997, 21:179-211.
38. Takahama K., Matsuoka M., Nagahama K., Ogawa T. Construction and analysis of a recombinant cyanobacterium expressing a chromosomally inserted gene for an ethylene-forming enzyme at the psbAI locus. J. Biosci. Bioeng. 2003, 95:302-305.
39. Takahashi H., Miyake M., Tokiwa Y., Asada Y. Improved accumulation of poly-3-hydroxybutyrate by recombinant cyanobacteria. Biotechnol. Lett. 1998, 20:183-186.
40. 2 to ethylene in recombinant cyanobacterium Synechocystis 6803. Energy Environ. Sci. 2012, 5:8998-9006.
41. 2. Metab. Eng. 2013, 16:68-77.
42. Wiesenborn D.P., Rudolph F.B., Papoutsakis E.T. Thiolase from Clostridium acetobutylicum ATCC 824 and its role in the synthesis of acids and solvents. Appl. Environ. Microbiol. 1988, 54:2717-2722.
43. Wiesenborn D.P., Rudolph F.B., Papoutsakis E.T. Coenzyme A transferase from Clostridium acetobutylicum ATCC 824 and its role in the uptake of acids. Appl. Environ. Microbiol. 1989, 55:323-329.
44. Zhou J., Zhang H., Zhang Y., Li Y., Ma Y. Designing and creating a modularized synthetic pathway in cyanobacterium Synechocysits enables production of acetone from carbon dioxide. Metab. Eng. 2012, 14:394-400.