Engineering Escherichia coli for malate production by integrating modular pathway characterization with CRISPRi-guided multiplexed metabolic tuning

Biotechnology and Bioengineering

Volumn 115, Issue 3, 2018, Pages 661-672

  Gao, Cong ^a   Wang, Shihui ^a   Hu, Guipeng ^a   Guo, Liang ^a   Chen, Xiulai ^a   Xu, Peng ^b   Liu, Liming ^a  

^a JIANGNAN UNIVERSITY   (China)

^b University of Marylandw Baltimore County   (United States)

Author keywords

CRISPRi; glyoxylate cycle; in vitro modular optimization; multiplexed combinatorial regulation

Indexed keywords

BIOSYNTHESIS; ESCHERICHIA COLI; GENE EXPRESSION; METABOLISM;

BIOCATALYTIC REACTION; COMBINATORIAL ENGINEERING; CRISPRI; FED-BATCH CULTIVATION; GENE EXPRESSION PATTERNS; GLYOXYLATE CYCLES; MODULAR OPTIMIZATIONS; MULTIPLEXED COMBINATORIAL REGULATION;

METABOLIC ENGINEERING;

GLUCOSE; GLYOXYLIC ACID; MALIC ACID; MALIC ACID DERIVATIVE;

ARTICLE; BACTERIAL STRAIN; CATALYTIC EFFICIENCY; CELL FREE SYSTEM; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEAT; CONTROLLED STUDY; ESCHERICHIA COLI; GENE EXPRESSION; IN VITRO STUDY; IN VIVO STUDY; METABOLIC ENGINEERING; NONHUMAN; CRISPR CAS SYSTEM; GENETICS; METABOLISM;

CRISPR-CAS SYSTEMS; ESCHERICHIA COLI; MALATES; METABOLIC ENGINEERING;

EID: 85034750457     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.26486     Document Type: Article

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